Resveratrol
(Synonyms: 白藜芦醇; trans-Resveratrol; SRT501) 目录号 : GC14553
白藜芦醇（转-白藜芦醇；SRT501）是一种植物抗菌素，具有强大的还原剂作用
Cas No.:501-36-0
Sample solution is provided at 25 µL, 10mM.
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| Cell experiment [1]: | |
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Cell lines |
ALVA-41, PC-3, and BPH-1 cells |
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Preparation Method |
For cell viability studies, ALVA-41, PC-3, and BPH-1 cells were treated for 24 h with 10 µmol/L and 100 µmol/L concentrations of resveratrol or EGCG. |
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Reaction Conditions |
10, 100 µM for 24 hours |
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Applications |
Resveratrol at low doses (10 µmol/L) induced a proliferative response in ALVA-41 and BPH-1 cells at 24 h, which was not evident in PC-3 cells. However, at a higher dose (100 µmol/L), resveratrol produced a significant toxicity in prostate cancer cells, although the effect on ALVA-41 cells was relatively greater than on PC-3 cells. |
| Animal experiment [2]: | |
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Animal models |
CB17SC mice model injected with LAPC-4 cells |
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Preparation Method |
In the primary LAPC-4 study, mice were fed no resveratrol (control), 50 mg/kg/day resveratrol (RV50), or 100 mg/kg/day resveratrol (RV100). |
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Dosage form |
Fed in diet, 50 mg/kg/day and 100 mg/kg/day |
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Applications |
In the LAPC-4 study, RV50 significantly decreased survival while RV100 did not significantly change survival. |
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References: [1]: Ahmad K A, Harris N H, Johnson A D, et al. Protein kinase CK2 modulates apoptosis induced by resveratrol and epigallocatechin-3-gallate in prostate cancer cells[J]. Molecular cancer therapeutics, 2007, 6(3): 1006-1012. |
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Resveratrol (trans-Resveratrol; SRT501) is a phytoalexin. Resveratrol is a potent reducing agent, and can prevent carcinogenesis due to its anti-oxidant abilities [1]. Resveratrol has a broad range of targets, including cyclooxygenase (e.g., COX, IC50=1.1 μM), lipoxygenase (LOC, IC50=2.7 μM), STAT3 (IC50=20 μM), and other proteins [2,3].
Resveratrol treatment is found to exert its effect on renal cell carcinoma (RCC) proliferation, migration and invasion in a concentration dependent manner through inactivation of the Akt and ERK1/2 signaling pathways [4]. In CaCo-2 cells, treatment with 25 μM Resveratrol has shown 70% growth inhibition due to S/G2 phase arrest [5]. Resveratrol treatment lead to inhibited invasion and metastasis of colorectal cancer-derived cell lines LoVo and HCT116 by suppressing the Wnt/β-catenin signaling mediated target genes of c-Myc, MMP-7, and MALT-1[6].
Resveratrol is shown to be effective against breast cancer metastasis to lungs in mice by its inhibitory effect on Stat3 mediated signaling [7]. Resveratrol treatment reduced size and number of tumor spheres in renal carcinoma stem cells xenograft mice model [8]. Resveratrol treatment reduced Epithelial to mesenchymal transition (EMT) of glioblastoma U87 xenografted mice models [9].
白藜芦醇（转-白藜芦醇；SRT501）是一种植物抗菌素。它具有强大的还原剂作用，可以通过其抗氧化能力预防致癌物质的形成[1]。 白藜芦醇具有广泛的靶点，包括环氧合酶（例如COX，IC50 = 1.1μM），脂肪氧合酶（LOC，IC50 = 2.7μM），STAT3（IC50 = 20μM）和其他蛋白质[2,3]。
翻译：研究发现，白藜芦醇治疗可以通过抑制Akt和ERK1/2信号通路的活性，在浓度依赖性下对肾细胞癌（RCC）增殖、迁移和侵袭产生影响。在CaCo-2细胞中，使用25μM的白藜芦醇处理可导致70%的生长抑制，原因是S/G2期阻滞。同时，白藜芦醇治疗还能通过抑制Wnt/β-catenin信号介导的靶基因c-Myc、MMP-7和MALT-1来抑制结直肠癌衍生细胞系LoVo和HCT116的侵袭和转移。
翻译：据显示，白藜芦醇通过抑制Stat3介导的信号传递，在小鼠乳腺癌转移至肺部方面具有一定的效果。在肾癌干细胞异种移植小鼠模型中，白藜芦醇治疗减少了肿瘤球的大小和数量。在人类胶质母细胞瘤U87异种移植小鼠模型中，白藜芦醇治疗减少了上皮向间充质转化（EMT）。
References:
[1]. Bhaskara V K, Mittal B, Mysorekar V V, et al. Resveratrol, cancer and cancer stem cells: A review on past to future[J]. Current Research in Food Science, 2020, 3: 284-295.
[2]. Calamini B, Ratia K, Malkowski M G, et al. Pleiotropic mechanisms facilitated by resveratrol and its metabolites[J]. Biochemical Journal, 2010, 429(2): 273-282.
[3]. Pirola L, Fr?jd? S. Resveratrol: one molecule, many targets[J]. IUBMB life, 2008, 60(5): 323-332.
[4]. Zhao Y, Tang H, Zeng X, et al. Resveratrol inhibits proliferation, migration and invasion via Akt and ERK1/2 signaling pathways in renal cell carcinoma cells[J]. Biomedicine & Pharmacotherapy, 2018, 98: 36-44.
[5]. Schneider Y, Vincent F, Duranton B, et al. Anti-proliferative effect of resveratrol, a natural component of grapes and wine, on human colonic cancer cells[J]. Cancer letters, 2000, 158(1): 85-91.
[6]. Ji Q, Liu X, Fu X, et al. Resveratrol inhibits invasion and metastasis of colorectal cancer cells via MALAT1 mediated Wnt/β-catenin signal pathway[J]. PloS one, 2013, 8(11): e78700.
[7]. Lee-Chang C, Bodogai M, Martin-Montalvo A, et al. Inhibition of breast cancer metastasis by resveratrol-mediated inactivation of tumor-evoked regulatory B cells[J]. The Journal of Immunology, 2013, 191(8): 4141-4151.
[8]. Ji Q, Liu X, Fu X, et al. Resveratrol inhibits invasion and metastasis of colorectal cancer cells via MALAT1 mediated Wnt/β-catenin signal pathway[J]. PloS one, 2013, 8(11): e78700.
[9]. Song Y, Chen Y, Li Y, et al. Resveratrol suppresses epithelial-mesenchymal transition in GBM by regulating Smad-dependent signaling[J]. BioMed Research International, 2019, 2019.
| Cas No. | 501-36-0 | SDF | |
| 别名 | 白藜芦醇; trans-Resveratrol; SRT501 | ||
| 化学名 | 5-[(E)-2-(4-hydroxyphenyl)ethenyl]benzene-1,3-diol | ||
| Canonical SMILES | C1=CC(=CC=C1C=CC2=CC(=CC(=C2)O)O)O | ||
| 分子式 | C14H12O3 | 分子量 | 228.24 |
| 溶解度 | ≥ 100mg/mL in DMSO；≥ 50mg/mL in EtOH | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.3814 mL | 21.9068 mL | 43.8135 mL |
| 5 mM | 0.8763 mL | 4.3814 mL | 8.7627 mL |
| 10 mM | 0.4381 mL | 2.1907 mL | 4.3814 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Influence of Resveratrol on the Immune Response
Resveratrol is the most well-known polyphenolic stilbenoid, present in grapes, mulberries, peanuts, rhubarb, and in several other plants. Resveratrol can play a beneficial role in the prevention and in the progression of chronic diseases related to inflammation such as diabetes, obesity, cardiovascular diseases, neurodegeneration, and cancers among other conditions. Moreover, resveratrol regulates immunity by interfering with immune cell regulation, proinflammatory cytokines' synthesis, and gene expression. At the molecular level, it targets sirtuin, adenosine monophosphate kinase, nuclear factor-κB, inflammatory cytokines, anti-oxidant enzymes along with cellular processes such as gluconeogenesis, lipid metabolism, mitochondrial biogenesis, angiogenesis, and apoptosis. Resveratrol can suppress the toll-like receptor (TLR) and pro-inflammatory genes' expression. The antioxidant activity of resveratrol and the ability to inhibit enzymes involved in the production of eicosanoids contribute to its anti-inflammation properties. The effects of this biologically active compound on the immune system are associated with widespread health benefits for different autoimmune and chronic inflammatory diseases. This review offers a systematic understanding of how resveratrol targets multiple inflammatory components and exerts immune-regulatory effects on immune cells.
Health benefits of resveratrol administration
Resveratrol is a polyphenol that is abundant in grape skin and seeds. Food sources of resveratrol include wine, berries, and peanuts. This compound has many properties, including activity against glycation, oxidative stress, inflammation, neurodegeneration, several types of cancer, and aging. Because resveratrol is generally welltolerated, it is believed to be a promising compound in preventing many diseases, such as diabetes and its complications. Unfortunately, this compound exhibits low bioavailability and solubility. The aim of this review is to summarize the latest information on the multiple effects of resveratrol on health and the benefits of its intake, based on in vitro and in vivo studies in animals and humans.
Resveratrol and Its Effects on the Vascular System
Resveratrol, the phenolic substance isolated initially from Veratrum grandiflorum and richly present in grapes, wine, peanuts, soy, and berries, has been attracting attention of scientists and medical doctors for many decades. Herein, we review its effects on the vascular system. Studies utilizing cell cultures and pre-clinical models showed that resveratrol alleviates oxidative stress and inflammation. Furthermore, resveratrol suppresses vascular smooth muscle cell proliferation, promotes autophagy, and has been investigated in the context of vascular senescence. Pre-clinical models unambiguously demonstrated numerous vasculoprotective effects of resveratrol. In clinical trials, resveratrol moderately diminished systolic blood pressure in hypertensive patients, as well as blood glucose in patients with diabetes mellitus. Yet, open questions remain, as exemplified by a recent report which states that the intake of resveratrol might blunt certain positive effects of exercise in older persons, and further research addressing the framework for long-term use of resveratrol as a food supplement, will stay in demand.
Anti-Inflammatory Action and Mechanisms of Resveratrol
Resveratrol (3,4',5-trihy- droxystilbene), a natural phytoalexin polyphenol, exhibits anti-oxidant, anti-inflammatory, and anti-carcinogenic properties. This phytoalexin is well-absorbed and rapidly and extensively metabolized in the body. Inflammation is an adaptive response, which could be triggered by various danger signals, such as invasion by microorganisms or tissue injury. In this review, the anti-inflammatory activity and the mechanism of resveratrol modulates the inflammatory response are examined. Multiple experimental studies that illustrate regulatory mechanisms and the immunomodulatory function of resveratrol both in vivo and in vitro. The data acquired from those studies are discussed.
Resveratrol in Various Pockets: A Review
Several phenolic compounds bind to proteins (such as enzymes) and interfere in their catalytic mechanism. Interaction studies of natural polyphenol; Resveratrol with various targets like with tubulin, protein kinase C alpha (PKCα), phosphodiesterase-4D, human oral cancer cell line proteins, DNA sequences having AATT/TTAA segments, protein kinase C alpha, lysine-specific demethylase 1 have been reviewed in this article. Simulation studies indicate that resveratrol and its analogs/ derivatives show good interaction with the target receptor through its hydroxyl groups by forming hydrogen bonds and hydrophobic interactions with amino acid residues at the binding site. Binding geometry and stability of complex formed by resveratrol show that it is a good inhibitor for many pathogenic targets. Further studies in this direction is, however, the need of the hour to develop many more ligands based on resveratrol skeleton which can further serve in the treatment of ailments.