N-Acetylserotonin
(Synonyms: N-乙酰-5-羟色胺,N-Acetyl-5-hydroxytryptamine,NAS) 目录号 : GC15365
The immediate precursor of melatonin
Cas No.:1210-83-9
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
|
Animal experiment: |
Mice[1] Two-month-old TrkB F616A mice are pretreated with 1NMPP1 in drinking water (50 μM) 1 day before the experiment, followed by administration of N-Acetyl-5-hydroxytryptamine (20 mg/kg, i.p.) or Melatonin (1 mg/kg, i.p.). Mice are killed at 1 h. The brain homogenates are analyzed by immunoblotting with anti-p-TrkB. Two- to three-month-old BDNF forebrain conditional knockout mice are injected i.p. with N-Acetyl-5-hydroxytryptamine or Melatonin. Mice are killed at 0, 0.5, 1, or 2 h following drug administration. Brain lysates are prepared and analyzed by immunoblotting with anti-phospho-TrkB Y816[1]. |
|
References: [1]. Jang SW, et al. N-acetylserotonin activates TrkB receptor in a circadian rhythm. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3876-81. |
|
N-Acetylserotonin is an agonist at the melatonin receptors MT1, MT2, and MT3.
A melatonin receptor is a G protein-coupled receptor binding melatonin. Three types of melatonin receptor have been identified. The MT1 and MT2 receptor subtypes are in humans and other mammals, whereas an additional melatonin receptor subtype MT3 has been identified in amphibia and birds.
In vitro: N-Acetylserotonin, a precursor of melatonin, was acetylated from serotonin by arylalkylamine Nacetyltransferase (AANAT). N-Acetylserotonin was found to be able to swiftly activate TrkB in a circadian manner. N-Acetylserotonin also exhibited antidepressant effect in a TrkB-dependent manner. In additioin, N-acetylserotonin could rapidly activate TrkB, but not TrkA or TrkC, in a neurotrophin- and MT3 receptor-independent manner. Moreover, N-acetylserotonin, but not melatonin, showed a robust antidepressant-like behavioral effect in a TrkB-dependent way [1].
In vivo: Animal study found that in BDNF knockout mice the administration of N-acetylserotonin could activate TrkB. Moreover, the endogenous TrkB was activated in wild-type C3H/f+/+ mice but not in AANAT-mutated C57BL/6J mice, in a circadian rhythm. In addition, TrkB activation was found to be high at night in the dark and low during the day [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Jang, S. W.,Liu, X.,Pradoldej, S., et al. N-acetylserotonin activates TrkB receptor in a circadian rhythm. Proceedings of the National Academy of Sciences of the United States of America 107(8), 3876-3881 (2010).
| Cas No. | 1210-83-9 | SDF | |
| 别名 | N-乙酰-5-羟色胺,N-Acetyl-5-hydroxytryptamine,NAS | ||
| 化学名 | N-[2-(5-hydroxy-1H-indol-3-yl)ethyl]-acetamide | ||
| Canonical SMILES | OC1=CC=C(NC=C2CCNC(C)=O)C2=C1 | ||
| 分子式 | C12H14N2O2 | 分子量 | 218.3 |
| 溶解度 | ≥ 11.5mg/mL in ethanol | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 4.5809 mL | 22.9043 mL | 45.8085 mL |
| 5 mM | 0.9162 mL | 4.5809 mL | 9.1617 mL |
| 10 mM | 0.4581 mL | 2.2904 mL | 4.5809 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。