Sucralose-d6
(Synonyms: SUCRALOSE-D6标准品,E955-d6; Trichlorosucrose-d6) 目录号 : GC48112
An internal standard for the quantification of sucralose
Cas No.:1459161-55-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Sucralose-d6 contains six deuterium atoms. It is intended for use as an internal standard for the quantification of sucralose by GC- or LC-MS.1,2 Sucralose is a non-caloric chlorinated disaccharide used as a sugar alternative that is not metabolized by humans.3 Excreted sucralose collects in wastewater and is considered a pollutant.1,2
1.Wu, M., Qian, Y., Boyd, J.M., et al.Direct large volume injection ultra-high performance liquid chromatography-tandem mass spectrometry determination of artificial sweeteners sucralose and acesulfame in well waterJ. Chromatogr. A.1359156-161(2014) 2.Ferrer, I., Zweigenbaum, J.A., and Thurman, E.M.Analytical methodologies for the detection of sucralose in waterAnal. Chem.85(20)9581-9587(2013) 3.Magnuson, B.A., Roberts, A., and Nestmann, E.R.Critical review of the current literature on the safety of sucraloseFood. Chem. Toxicol.106(Pt A)324-355(2017)
| Cas No. | 1459161-55-7 | SDF | |
| 别名 | SUCRALOSE-D6标准品,E955-d6; Trichlorosucrose-d6 | ||
| Canonical SMILES | Cl[C@@H]1[C@H](O)[C@@H](O)[C@@H](O[C@@]2(C([2H])([2H])Cl)[C@@H](O)[C@H](O)[C@@H](C([2H])([2H])Cl)O2)O[C@@H]1C([2H])([2H])O | ||
| 分子式 | C12H13Cl3D6O8 | 分子量 | 403.7 |
| 溶解度 | Methanol: Slightly Soluble,Water: Slightly Soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4771 mL | 12.3854 mL | 24.7709 mL |
| 5 mM | 0.4954 mL | 2.4771 mL | 4.9542 mL |
| 10 mM | 0.2477 mL | 1.2385 mL | 2.4771 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Analytical methodologies for the detection of sucralose in water
Anal Chem 2013 Oct 15;85(20):9581-7.PMID:24006925DOI:10.1021/ac4016984
A detailed evaluation of two liquid chromatography-mass spectrometry techniques, quadrupole time-of-flight mass spectrometry (LC/Q-TOF-MS) and triple quadrupole mass spectrometry (LC/MS-MS), was carried out in terms of sensitivity, selectivity, and ionization mode for the detection of sucralose in environmental water samples, which is an important environmental topic in water analysis. Interesting findings were made in regards to fragmentation and sensitivity when both techniques and both ionization modes of operation were compared. In positive ion mode, sucralose was detected by its sodium adduct [M + Na](+) at m/z 419.0038. Fragmentation by MS-MS of the sodiated molecule was possible using either LC/MS-MS or LC/Q-TOF-MS under positive ionization. Accurate mass measurements provided exact structural confirmation of the sodiated fragments obtained (m/z 221.0187 and m/z 238.9848). In negative ion mode, the deprotonated molecule was observed ([M - H](-) at m/z 395.0073), and fragmentation by MS-MS yielded two characteristic fragment ions (m/z 359.0306 and m/z 34.9694). Because sucralose contains three chlorine atoms, time-of-flight analyses provided a valuable amount of isotopic accurate mass information for its detection. With LC/MS-MS, the sensitivity was 10 times higher in positive ion mode than in negative ion mode, with limits of detection of 15 ng/L. Similarly, when time-of-flight mass spectrometry was used, the sensitivity was slightly better in positive ion mode, with limits of detection of 400 ng/L. Matrix effects were observed for surface and wastewater samples; thus the use of a deuterated standard (Sucralose-d6) was crucial for precise quantitation. The most sensitive analytical methodology for the analysis of sucralose in water samples was LC/MS-MS using triple quadrupole under positive ion mode.