Psammaplin A
目录号 : GC60310PsammaplinA是一种海洋代谢产物,是HDAC和DNA甲基转移酶(DNAmethyltransferases)的有效抑制剂。PsammaplinA是一种高效的选择性HDAC1抑制剂,IC50为0.9nM,对HDAC1的选择性是DAC6的360倍,对HDAC7和HDAC8的效力低1000倍以上。PsammaplinA对革兰氏阳性细菌(Gram-positivebacteria)具有抗菌作用,并抑制DNA合成和DNA促旋酶(DNAgyrase)活性。抗肿瘤活性。
Cas No.:110659-91-1
Sample solution is provided at 25 µL, 10mM.
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Psammaplin A, a marine metabolite, is a potent inhibitor of HDAC and DNA methyltransferases. Psammaplin A ia a highly potent and selective DAC1 inhibitor with an IC50 of 0.9 nM. Psammaplin A possess the antimicrobial effect on the Gram-positive bacteria and inhibits DNA synthesis and DNA gyrase activity. Antitumor Activity[1][2].
[1]. Matthias G J Baud, et al. Defining the Mechanism of Action and Enzymatic Selectivity of Psammaplin A Against Its Epigenetic Targets. J Med Chem. 2012 Feb 23;55(4):1731-50. [2]. Psammaplin A, a Natural Bromotyrosine Derivative From a Sponge, Possesses the
Cas No. | 110659-91-1 | SDF | |
Canonical SMILES | O=C(NCCSSCCNC(/C(CC1=CC(Br)=C(O)C=C1)=N/O)=O)/C(CC2=CC(Br)=C(O)C=C2)=N/O | ||
分子式 | C22H24Br2N4O6S2 | 分子量 | 664.39 |
溶解度 | 储存条件 | 4°C, stored under nitrogen | |
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1 mg | 5 mg | 10 mg | |
1 mM | 1.5051 mL | 7.5257 mL | 15.0514 mL |
5 mM | 0.301 mL | 1.5051 mL | 3.0103 mL |
10 mM | 0.1505 mL | 0.7526 mL | 1.5051 mL |
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Marine-Derived Natural Lead Compound Disulfide-Linked Dimer Psammaplin A: Biological Activity and Structural Modification
Mar Drugs 2019 Jun 27;17(7):384.PMID:31252563DOI:10.3390/md17070384.
Marine natural products are considered to be valuable resources that are furnished with diverse chemical structures and various bioactivities. To date, there are seven compounds derived from marine natural products which have been approved as therapeutic drugs by the U.S. Food and Drug Administration. Numerous bromotyrosine derivatives have been isolated as a type of marine natural products. Among them, Psammaplin A, including the oxime groups and carbon-sulfur bonds, was the first identified symmetrical bromotyrosine-derived disulfide dimer. It has been found to have a broad bioactive spectrum, especially in terms of antimicrobial and antiproliferative activities. The highest potential indole-derived Psammaplin A derivative, UVI5008, is used as an epigenetic modulator with multiple enzyme inhibitory activities. Inspired by these reasons, Psammaplin A has gradually become a research focus for pharmacologists and chemists. To the best of our knowledge, there is no systematic review about the biological activity and structural modification of Psammaplin A. In this review, the pharmacological effects, total synthesis, and synthesized derivatives of Psammaplin A are summarized.
Psammaplin A as a general activator of cell-based signaling assays via HDAC inhibition and studies on some bromotyrosine derivatives
Bioorg Med Chem 2009 Mar 15;17(6):2189-98.PMID:19022675DOI:10.1016/j.bmc.2008.10.077.
The Wnt signaling pathway regulates cell growth and development in metazoans, and is therefore of interest for drug discovery. By screening a library of 5808 pre-fractionated marine extracts in a cell-based Wnt signaling assay, several signaling activators and inhibitors were observed. LCMS-based fractionation rapidly identified an active compound from Pseudoceratina purpurea as Psammaplin A, a known HDAC inhibitor. Other HDAC inhibitors similarly activated signaling in this assay, indicating HDAC inhibitors will be identified through many cell-based reporter assays. In a large scale analysis of P. purpurea, three previously undescribed bromotyrosine based natural products were identified; the structure of one of these was confirmed by synthesis. Additionally, three other derivatives of Psammaplin A were prepared: a mixed disulfide and two sulfinate esters. Finally, evidence to support a structural reassignment of psammaplin I from a sulfone to the isomeric sulfinate ester is presented.
Cytotoxicity of Psammaplin A from a two-sponge association may correlate with the inhibition of DNA replication
BMC Cancer 2004 Sep 30;4:70.PMID:15456519DOI:10.1186/1471-2407-4-70.
Background: SV40 DNA replication system is a very useful tool to understand the mechanism of replication, which is a tightly regulated process. Many environmental and cellular factors can induce cell cycle arrest or apoptosis by inhibiting DNA replication. In the course of our search for bioactive metabolites from the marine sponges, Psammaplin A was found to have some anticancer properties, the possible mechanism of which was studied. Methods: Cell viability was determined by Cell Counting Kit-8 (CCK-8) to count living RAW264.7 cells by combining 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) and 1-methoxy-phenazine methosulfate (1-methoxy-PMS). The effect of Psammaplin A on DNA replication was carried out in SV40 DNA replication system in vitro. The activities of topoisomerase I and polymerase alpha-primase were measured by the relaxation of superhelical plasmid DNA and the incorporation of [3H]dTTP to the template respectively. The ssDNA binding activity of RPA was assessed by Gel Mobility Shift Assay (GMSA). Results: We have found that Psammaplin A delivers significant cytotoxic activity against the RAW264.7 cell line. It was also found that Psammaplin A could substantially inhibit SV40 DNA replication in vitro, in which polymerase alpha-primase is one of its main targets. Conclusion: Taken together, we suggest that psammaplin A-induced cytotoxicity may correlate with its inhibition on DNA replication. Psammaplin A has the potential to be developed as an anticancer drug.
Camptothecin-psammaplin A hybrids as topoisomerase I and HDAC dual-action inhibitors
Eur J Med Chem 2018 Jan 1;143:2005-2014.PMID:29150335DOI:10.1016/j.ejmech.2017.11.021.
Recent studies have demonstrated enhanced anticancer effects of combination therapy consisting of camptothecin derivatives and HDAC inhibitors. To exploit this synergy in a single active compound, we designed new dual-acting multivalent molecules simultaneously targeting topoisomerase I and HDAC. In particular, a selected compound containing a camptothecin and the Psammaplin A scaffold showed a broad spectrum of antiproliferative activity, with IC50 values in the nanomolar range. Preliminary in vivo results indicated a strong antitumor activity on human mesothelioma primary cell line MM473 orthotopically xenografted in CD-1 nude mice and very high tolerability.
Psammaplin A, a chitinase inhibitor isolated from the Fijian marine sponge Aplysinella rhax
Bioorg Med Chem 2002 Apr;10(4):1123-8.PMID:11836123DOI:10.1016/s0968-0896(01)00372-8.
Several brominated tyrosine derived compounds, psammaplins A (1), K (2) and L (3) as well as bisaprasin (4) were isolated from the Fijian marine sponge Aplysinella rhax during a bioassay guided isolation protocol. Their structures were determined using NMR and MS techniques. Psammaplin A was found to moderately inhibit chitinase B from Serratia marcescens, the mode of inhibition being non-competitive. Crystallographic studies suggest that a disordered Psammaplin A molecule is bound near the active site. Interestingly, Psammaplin A was found to be a potent antifungal agent.