Pregnenolone
(Synonyms: 孕烯醇酮; 3β-Hydroxy-5-pregnen-20-one) 目录号 : GC12920
A natural steroid hormone
Cas No.:145-13-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Animal experiment: |
Mice and Rats[1]Adult male Wistar rats (weighing 320-340g), Sprague Dawley male rats (weighing 330-350g), C57BL/6N mice (2-3 months) and CD1 mice (weighing 25-30 g at the beginning of the experiments) are used. Pregnenolone is injected subcutaneously (sc). The injection volumes are 1 mL/kg of body weight for rats and 10 mL/kg for mice[1]. |
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References: [1]. Vallée M, et al. Pregnenolone can protect the brain from cannabis intoxication. Science. 2014 Jan 3;343(6166):94-8. |
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Neurosteroids are endogenous molecules that are enrichedin human brain. A number of neurosteroids demonstrate important pleiotropic actions that impact critical brainfunctions at physiologically relevant concentrations in rodent models. Pregnenolone (3β-hydroxypregn-5-en-20-one), also known as P5, is an endogenous steroid hormone.
In vitro: Pregnenolone is the precursor from which almost all of the other steroid hormones are made, including DHEA, testosterone, progesterone, the estrogens and cortisol. Pregnenolone also operates as a powerful neurosteroid in the brain, which modulates the transmission of messages from neuron to neuron and strongly influencing learning and memory processes [1].
In vivo: The ability of pregnenolone sulfate derivatives (PREGS) to modulate the age-induced learning impairment was tested in 16- month-old mice using the step-down type of passive avoidance. Decreased step-down latency was observed in the passive avoidance task in 16-month-old mice compared to 3-month-old mice, revealing retention deficits in old mice. Pretraining injections of PREGS dose-dependently improved the 24 h delay retention performances in this task in old mice [1].
Clinical trial: Human studies have reported improvement of learning and memory dysfunction after PREG administration to individuals with low PREGS levels, but other studies failed to detect significant cognitive effects of PREG administration [1].
Reference:
[1] Vallée M, Mayo W, Le Moal M. Role of pregnenolone, dehydroepiandrosterone and their sulfate esters on learning and memory in cognitive aging. Brain Res Brain Res Rev. 2001 Nov;37(1-3):301-12.
| Cas No. | 145-13-1 | SDF | |
| 别名 | 孕烯醇酮; 3β-Hydroxy-5-pregnen-20-one | ||
| 化学名 | 1-[(3S,8S,9S,10R,13S,14S,17S)-3-hydroxy-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-17-yl]ethanone | ||
| Canonical SMILES | CC(=O)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)O)C)C | ||
| 分子式 | C21H32O2 | 分子量 | 316.48 |
| 溶解度 | ≥ 15.824mg/mL in DMSO with gentle warming | 储存条件 | Store at 4°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.1598 mL | 15.7988 mL | 31.5976 mL |
| 5 mM | 0.632 mL | 3.1598 mL | 6.3195 mL |
| 10 mM | 0.316 mL | 1.5799 mL | 3.1598 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。