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NMS-859 Sale

目录号 : GC32874

NMS-859是一种有效的,共价的VCP(p97)抑制剂,细胞中分别在60μM和1mMATP的条件下,对野生型VCP的IC50值分别为0.37和0.36μM。

NMS-859 Chemical Structure

Cas No.:1449236-96-7

规格 价格 库存 购买数量
10mM (in 1mL DMSO)
¥4,006.00
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2mg
¥2,082.00
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5mg
¥3,124.00
现货
10mg
¥4,463.00
现货
50mg
¥13,388.00
现货
100mg
¥18,743.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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实验参考方法

Cell experiment:

Cells are seeded at 1,600 cells per well in 384-well white clear-bottom plates. Twenty-four hours after seeding, cells are treated with NMS-859 (eight dilution points, in duplicate) and incubated for an additional 72 h at 37°C under a 5% CO2 atmosphere. Cells are then lysed, and the ATP content in each well is determined using a thermostable firefly luciferase-based assay as a measure of cell viability. IC50 values are calculated using the percentage of growth of treated cells versus the untreated control[1].

References:

[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

产品描述

NMS-859 is a potent, covalent VCP (p97) inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively.

NMS-859 is a potent VCP inhibitor, with IC50s of 0.37 and 0.36 μM for wild-type VCP in the presence of 60 μM and 1 mM ATP in cells, respectively. NMS-859 shows very weak inhibitory activity against VCPC522T. NMS-859 also suppresses the proliferation of cells, with IC50s of 3.5 μM and 3.0 μM in HCT116 and HeLa cell lines, respectively[1].

[1]. Magnaghi P, et al. Covalent and allosteric inhibitors of the ATPase VCP/p97 induce cancer cell death. Nat Chem Biol. 2013 Sep;9(9):548-56.

Chemical Properties

Cas No. 1449236-96-7 SDF
Canonical SMILES O=S1(C2=CC=CC=C2C(NC3=CC(NC(CCl)=O)=CC=C3)=N1)=O
分子式 C15H12ClN3O3S 分子量 349.79
溶解度 DMSO : ≥ 42 mg/mL (120.07 mM) 储存条件 Store at -20°C
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储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
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1 mg 5 mg 10 mg
1 mM 2.8589 mL 14.2943 mL 28.5886 mL
5 mM 0.5718 mL 2.8589 mL 5.7177 mL
10 mM 0.2859 mL 1.4294 mL 2.8589 mL
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