|(-)-epicatechin gallate 目录号 GC10603|
Sample solution is provided at 25 µL, 10mM.
RatsMale Sprague-Dawley rats, obtained at 7 weeks of age (210-245 g) are used. Epicatechin gallate (ECG) is suspended in 0.5% w/v sodium carboxymethylcellulose at 12.5, 25.0 and 50.0 mg/10 mL for oral administration to rats at 10 mL/kg. For intravenous injection in rats at 1.0 mg/kg, an ethanolic solution of Epicatechin gallate is diluted with 10% w/v sodium citrate solution to 1.0 mg/mL; the final concentration of ethanol is 1% v/v.
. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9.
|溶解度||≥ 22.1mg/mL in DMSO||储存条件||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
(-)-epicatechin gallate is a major catechin component in green tea .
(-)-epicatechin gallate (ECG) plays an important role in cell growth inhibition, apoptosis and membrane transport system .
(-)-epicatechin gallate is a kind of catechin. In HCT-116 cells, ECG activated transcription factor 3 (ATF3), which played a critical role in pro-apoptosis. EGR-1 was involved in ECG-induced ATF3 expression. In HCT-116 cells, ECG (50 µM) increased NAG-1 and ATF3 expression in time- and dose-dependent way . In carcinoma HSC-2 cells, ECG (50 µM) exhibited cytotoxicity with midpoint cytotoxicity (NR50) value of 67 µM. However, in normal HGF-2 fibroblasts, ECG exhibited cytotoxicity at concentrations up to 25 µM with NR50 value of 100 µM. In carcinoma HSC-2 cells, ECG (250 µM) induced nucleosomal DNA fragmentation and apoptosis. ECG (150 µM) significantly increased caspase-3 activity .
In rats, there were five metabolites of ECG: (-)-epicatechin gallate, 3’,4’’-di-O-methyl-(-)-epicatechin gallate, 4’’-O-methyl-(-)-epicatechin gallate, 4’-O-methyl-(-)-epicatechin gallate and 3’-O-methyl-(-)-epicatechin gallate, which were excreted in rat urine .
. Cho KN, Sukhthankar M, Lee SH, et al. Green tea catechin (-)-epicatechin gallate induces tumour suppressor protein ATF3 via EGR-1 activation. Eur J Cancer, 2007, 43(16): 2404-2412.
. Babich H, Krupka ME, Nissim HA, et al. Differential in vitro cytotoxicity of (-)-epicatechin gallate (ECG) to cancer and normal cells from the human oral cavity. Toxicol In Vitro, 2005, 19(2): 231-242.
. Kohri T, Suzuki M, Nanjo F. Identification of metabolites of (-)-epicatechin gallate and their metabolic fate in the rat. J Agric Food Chem, 2003, 51(18): 5561-5566.