DNA/RNA Synthesis(DNA/RNA合成)
RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.
Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.
First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.
Products for DNA/RNA Synthesis
- Cat.No. 产品名称 Information
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GC63653
NCGC00029283
NCGC00029283 是一种 werner 综合征解旋酶-核酸酶 (WRN) 抑制剂,对 WRN、BLM 和 FANCJ 解旋酶的 IC50 分别为 2.3 μM、12.5 μM 和 3.4 μM。
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GC63123
NVS-SM2
NVS-SM2 是一种有效的、具有口服活性和可透过血脑屏障的 SMN2 剪接增强剂,对 SMN 的 EC50 为 2 nM。NVS-SM2 增强 U1-pre-mRNA 关联。NVS-SM2 促进外显子 7 包含并恢复正常存活运动神经元 (SMN) 蛋白的表达。NVS-SM2 可用于脊椎肌肉萎缩 (SMA) 的研究。
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GC62925
Deoxythymidine-5’-triphosphate trisodium salt
脱氧胸苷三磷酸三钠盐; dTTP trisodium
Deoxythymidine-5'-三磷酸 (dTTP) 三钠是用于合成 DNA 的四种核苷三磷酸之一。 -
GC62863
BCH001
BCH001 is a specific PAPD5 inhibitor that restores telomerase activity and telomere length in dyskeratosis congenita (DC) patient induced pluripotent stem cells. PAPD5 is a noncanonical poly(A) polymerase with an unusual RNA-binding motif.
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GC62813
5’-O-DMT-rI
5'-O-(4,4'-二甲氧基三苯甲基)肌苷
5’-O-DMT-Ri 可用于合成寡核苷酸。 -
GC62812
5’-O-DMT-PAC-dA
5'-O-[二(4-甲氧基苯基)苯基甲基]-2'-脱氧-N-(苯氧基乙酰基)腺苷
5’-O-DMT-PAC-dA 可用于合成寡核苷酸。 -
GC49384
L-Proline-d3
L-脯氨酸 d3
An internal standard for the quantification of L-proline -
GC49381
L-Leucine-d10
L-亮氨酸 d10
An internal standard for the quantification of L-leucine -
GC49368
L-Methionine-d3
L-蛋氨酸-d3
An internal standard for the quantification of L-methionine -
GC49348
2-Thiocytidine
2-硫代胞苷
A modified nucleobase -
GC49307
cDPCP
A DNA-crosslinking agent
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GC49297
Uridine-5’-monophosphate (sodium salt hydrate)
((2R,3S,4R,5R)-5-(2,4-二氧代-3,4-二氢嘧啶-1(2H)-基)-3,4-二羟基四氢呋喃-2-基)甲基磷酸二钠盐X水合物
A ribonucleotide -
GC49285
Adenosine 5’-methylenediphosphate (hydrate)
Adenosine 5'-(α,β-methylene)diphosphate, AMP-CP, APCP, 5'-APCP
An inhibitor of ecto-5’-nucleotidase -
GC49267
Hygromycin B-d4
An internal standard for the quantification of hygromycin
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GC49251
Oxaliplatin-d10
Lipoxal-d10
An internal standard for the quantification of oxaliplatin -
GC49245
5-Ethynyluridine
5-乙炔基尿苷,5-EU
A clickable form of uridine -
GC49231
Adenylosuccinic Acid (ammonium salt)
A purine nucleotide and an intermediate in the purine nucleotide cycle
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GC49225
Deoxyuridine 5′-monophosphate (sodium salt)
2'-脱氧尿苷-5'-单磷酸二钠
A nucleotide intermediate in the biosynthesis of dTMP -
GC49134
Flumequine-13C3
氟甲喹-13C3
An internal standard for the quantification of flumequine -
GC49132
Uracil-13C,15N2
尿嘧啶 13C2,15N2
An internal standard for the quantification of uracil -
GC49126
Folitixorin
5,10-亚甲基四氢叶酸(非对映体混合物),5,10-Methylenetetrafolate; ANX-510 free acid
Folitixorin是叶酸的一种还原形式,也是胸苷酸合成酶的辅助因子。 -
GC49104
Cytidine 3’-monophosphate
胞啶3'-单磷酸
A ribonucleotide -
GC49076
Tilmicosin-d3
LY-177370-d3; EL-870-d3
An internal standard for the quantification of tilmicosin -
GC49039
Althiomycin
NSC 102809
A thiazole antibiotic -
GC49033
Guanosine 5’-diphosphate (sodium salt hydrate)
GDP
A purine nucleotide -
GC49022
Didanosine-d2
去羟肌苷 d2
An internal standard for the quantification of didanosine -
GC49004
Adenosine 3’-monophosphate (sodium salt hydrate)
3’-AMP
A nucleotide -
GC48987
UDP (sodium salt hydrate)
Uridine-5'-diphosphate
An agonist of P2Y6 -
GC62693
SCR130
SCR130 is a novel DNA repair inhibitor with IC50s of 14 μM in Reh cells and 2 μM in Nalm6 cells. SCR130 specifically inhibits DNA Ligase IV‐mediated joining with minimal or no effect on Ligase III and Ligase I mediated joining.
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GC62679
NSAH
NSAH 是可逆的、竞争性的非核苷类的核苷酸还原酶 (RR)的抑制剂,其 cell-free 和 cell-bases 的 IC50 值分别为 32 μM 和 ~250 nM。
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GC62607
EFdA-TP tetraammonium
EFdA-TP tetraammonium 是一种有效的核苷逆转录酶 (RT) 抑制剂。EFdA-TP tetraammonium 作为有效的立即或延迟链终止剂 (ICT 或 DCT) 抑制 RT 催化的 DNA 合成。EFdA-TP tetraammonium 通过多种机制抑制 HIV-1 RT。
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GC62581
5’-O-TBDMS-dT
5'-O-(叔丁基二甲基甲硅烷基)胸苷
5’-O-TBDMS-dT 是一种具有保护和修饰作用的核苷。 -
GC62580
5’-O-TBDMS-dG
5'-O-(叔丁基二甲基甲硅烷基)脱氧鸟苷
5’-O-TBDMS-dG 是一种修饰的核苷酸,可用于脱氧核糖核酸或者核糖核酸的合成。 -
GC62579
5’-O-TBDMS-dA
5’-O-TBDMS-dA 是一种修饰的核苷,可以用于合成 DNA 或 RNA。
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GC62578
5’-O-TBDMS-Bz-dA
N-苯甲酰基-5'-O-叔丁基二甲基硅烷基-2'-脱氧腺苷
5’-O-TBDMS-Bz-dA 是一种具有保护和修饰作用的核苷。 -
GC62576
5’-O-DMT-N4-Bz-5-Me-dC
N-苯甲酰基-5'-O-[二(4-甲氧基苯基)苯基甲基]-2'-脱氧-5-甲基胞苷
5’-O-DMT-N4-Bz-5-Me-dC 是一种修饰的核苷酸,可用于脱氧核糖核酸或者核糖核酸的合成。 -
GC62575
5’-O-DMT-N4-Bz-2’-F-dC
5'-O-(4,4'-二甲氧基三苯甲基)-N4-苯甲酰-2'-氟-2'-脱氧胞苷
5’-O-DMT-N4-Bz-2’-F-Dc 是一种具有保护和修饰作用的核苷。 -
GC62574
5’-O-DMT-N4-Ac-2’-F-dC
N-乙酰基-5'-O-[双(4-甲氧基苯基)苯基甲基]-2'-脱氧-2'-氟胞苷
5’-O-DMT-3’-O-TBDMS-Ac-rC是一种修饰的核苷,可以用于合成 DNA 或 RNA。 -
GC62573
5’-O-DMT-Bz-rC
N-苯甲酰基-5'-O-[双(4-甲氧基苯基)苯甲基]胞苷
5’-O-DMT-Bz-Rc 是一种修饰的核苷,可以用于合成 DNA 或 RNA。 -
GC62547
5-O-TBDMS-N4-Benzoyl-2-deoxycytidine
N-苯甲酰基-5'-O-叔-丁基二甲基甲硅烷-2'-脱氧胞啶
5-O-TBDMS-N4-Benzoyl-2-deoxycytidine 是一种修饰的核苷酸,可用于脱氧核糖核酸或者核糖核酸的合成。 -
GC62546
Uridine triphosphate 13C9,15N2 sodium
尿苷-5'-三磷酸二钠盐,UTP 13C9,15N2 sodium; Uridine 5'-triphosphate 13C9,15N2 sodium
Uridine triphosphate 13C9,15N2 (UTP 13C9,15N2) sodium 是一种同位素标记的 Uridine triphosphate sodium。Uridine triphosphate sodium 可用于核酸合成。 -
GC62538
DMT-dG(dmf) Phosphoramidite
DMF-DG-CE亚磷酰胺单体
DMT-dG(dmf) Phosphoramidite 是一种亚磷酰胺单体,可用于寡核苷酸的制备。 -
GC62532
5’-O-DMT-3’-O-TBDMS-Ac-rC
5’-O-DMT-3’-O-TBDMS-Ac-rC 是一种修饰的核苷,可以用于合成 DNA 或 RNA。
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GC62531
2’-OMe-G(ibu) Phosphoramidite
5'-O-(4,4-二甲氧基三苯甲基)-2'-O-甲基-N-异丁酰基鸟苷-3'-(2-氰基乙基-N,N-二异丙基)亚磷酰胺
2’-OMe-G(ibu) Phosphoramidite 是一种修饰过的亚磷酰胺单体,可用于寡核苷酸的合成。 -
GC62530
2’-O-Me-C(Bz) Phosphoramidite
2'-OME-BZ-C亚磷酰胺单体
2’-O-Me-C(Bz) Phosphoramidite 是一种修饰过的亚磷酰胺单体,可用于寡核苷酸的合成。 -
GC62529
2’-OMe-A(Bz) Phosphoramidite
N-苯甲酰基-5'-O-(4,4-二甲氧基三苯甲基)-2'-O-甲基腺苷-3'-(2-氰基乙基-N,N-二异丙基)亚磷酰胺
2’-OMe-A(Bz) Phosphoramidite 是一种修饰过的亚磷酰胺单体,可用于寡核苷酸的合成。 -
GC62470
AG-636
A DHODH inhibitor
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GC62443
ErSO
ErSO activates the anticipatory unfolded protein response (a-UPR) with an IC50 of 20.3 nM in MCF-7 cells. ErSO induces rapid and selective necrosis of ERα-positive breast cancer cell lines in vitro.
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GC62389
5-Iminodaunorubicin
5-亚氨基柔红霉素
5-Iminodaunorubicin 是一种醌改性蒽环类药物,具有抗肿瘤活性。5-Iminodaunorubicin 在癌细胞中可诱导 DNA 链断裂。 -
GC62383
(±)-ErSO
(±)-ErSO 是 ErSO 的消旋体。ErSO 是选择性预期未折叠蛋白反应 (a-UPR) 激活物。