L-Leucine-d10
(Synonyms: L-亮氨酸 d10) 目录号 : GC49381An internal standard for the quantification of L-leucine
Cas No.:106972-44-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L-Leucine-d10 is intended for use as an internal standard for the quantification of L-leucine by GC- or LC-MS. L-Leucine is an essential amino acid.1 It increases basal insulin secretion and decreases glucose-induced insulin release in INS-1E rat insulinoma cells when used at concentrations of 1, 5, and 10 mM.2 L-Leucine (1, 5, and 10 mM) decreases triglyceride levels and increases cholesterol accumulation in INS-1E cells. It stimulates skeletal muscle protein synthesis in exercised rats, as well as in food-deprived rats in an mTOR-dependent manner.3,4 Formulations containing L-leucine have been used as dietary supplements.
1.Duan, Y., Li, F., Li, Y., et al.The role of leucine and its metabolites in protein and energy metabolismAmino Acids48(1)41-51(2016) 2.Liu, Z., Jeppesen, P.B., Gregersen, S., et al.Chronic exposure to leucine in vitro induces β-cell dysfunction in INS-1E cells and mouse isletsJ. Endocrinol.215(1)79-88(2012) 3.Anthony, J.C., Yoshizawa, F., Anthony, T.G., et al.Leucine stimulates translation initiation in skeletal muscle of postabsorptive rats via a rapamycin-sensitive pathwayJ. Nutr.130(10)2413-2419(2000) 4.Anthony, J.C., Anthony, T.G., and Layman, D.K.Leucine supplementation enhances skeletal muscle recovery in rats following exerciseJ. Nutr.129(6)1102-1106(1999)
Cas No. | 106972-44-5 | SDF | |
别名 | L-亮氨酸 d10 | ||
Canonical SMILES | [2H]C([2H])([2H])C(C([2H])([2H])[2H])([2H])C([2H])([2H])[C@@](N)([2H])C(O)=O | ||
分子式 | C6H3D10NO2 | 分子量 | 141.2 |
溶解度 | PBS (pH 7.2): 1 mg/ml | 储存条件 | -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 7.0822 mL | 35.4108 mL | 70.8215 mL |
5 mM | 1.4164 mL | 7.0822 mL | 14.1643 mL |
10 mM | 0.7082 mL | 3.5411 mL | 7.0822 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Specific labeling and assignment strategies of valine methyl groups for NMR studies of high molecular weight proteins
J Biomol NMR 2013 Nov;57(3):251-62.PMID:24078041DOI:10.1007/s10858-013-9785-z
The specific protonation of valine and leucine methyl groups in proteins is typically achieved by overexpressing proteins in M9/D2O medium supplemented with either labeled α-ketoisovalerate for the labeling of the four prochiral methyl groups or with 2-acetolactate for the stereospecific labeling of the valine and leucine side chains. However, when these labeling schemes are applied to large protein assemblies, significant overlap between the correlations of the valine and leucine methyl groups occurs, hampering the analysis of 2D methyl-TROSY spectra. Analysis of the leucine and valine biosynthesis pathways revealed that the incorporation of labeled precursors in the leucine pathway can be inhibited by the addition of exogenous L-Leucine-d10. We exploited this property to label stereospecifically the pro-R and pro-S methyl groups of valine with minimal scrambling to the leucine residues. This new labeling protocol was applied to the 468 kDa homododecameric peptidase TET2 to decrease the complexity of its NMR spectra. All of the pro-S valine methyl resonances of TET2 were assigned by combining mutagenesis with this innovative labeling approach. The assignments were transferred to the pro-R groups using an optimally labeled sample and a set of triple resonance experiments. This improved labeling scheme enables us to overcome the main limitation of overcrowding in the NMR spectra of prochiral methyl groups, which is a prerequisite for the site-specific measurement of the structural and dynamic parameters or for the study of interactions in very large protein assemblies.