Phosphatidylglycerols (sodium salt)
(Synonyms: 磷脂酸甘油酯) 目录号 : GC18377
Phosphatidylglycerols (sodium salt)是一种普遍存在于几乎所有生物体中的磷脂，分子结构特征为带负电荷的甘油头部基团，以钠盐形式稳定存在。
Cas No.:383907-64-0
Sample solution is provided at 25 µL, 10mM.
Phosphatidylglycerols (sodium salt) is a phospholipid that is ubiquitous in almost all living organisms, with molecular structure features a negatively charged glycerol head group, which exists stably in the form of a sodium salt[1]. Phosphatidylglycerols can inhibit the activation of Toll-like receptors (TLR) in the innate immune system of the lungs by microbial components, resulting in a reduction in the production of inflammatory mediators, and alleviation of lung inflammation and damage[2]. Phosphatidylglycerols have been widely used to enhance mitochondrial function and promote skin wound healing[3].
In vitro, Phosphatidylglycerols (50μM twice at 0 and 12 hours) significantly rescued the total oxygen consumption rates (OCR) and mitochondrial activity inhibited by Kdo2-Lipid A (KLA) in RAW264.7 cells[4].
References:
[1] Wada H, Murata N. The essential role of phosphatidylglycerol in photosynthesis[J]. Photosynthesis research, 2007, 92(2): 205-215.
[2] Bollag W B, Gonzales J N. Phosphatidylglycerol and surfactant: A potential treatment for COVID-19?[J]. Medical Hypotheses, 2020, 144: 110277.
[3] Luo Y, Vivaldi Marrero E, Choudhary V, et al. Phosphatidylglycerol to treat chronic skin wounds in diabetes[J]. Pharmaceutics, 2023, 15(5): 1497.
[4] Chen W W, Chao Y J, Chang W H, et al. Phosphatidylglycerol incorporates into cardiolipin to improve mitochondrial activity and inhibits inflammation[J]. Scientific reports, 2018, 8(1): 4919.
Phosphatidylglycerols (sodium salt)是一种普遍存在于几乎所有生物体中的磷脂，分子结构特征为带负电荷的甘油头部基团，以钠盐形式稳定存在[1]。Phosphatidylglycerols可通过抑制微生物成分对肺先天免疫系统中Toll样受体（TLR）的激活，减少炎症介质产生，减轻肺部炎症和损伤[2]。Phosphatidylglycerols被广泛应用于增强线粒体功能和促进皮肤伤口愈合[3]。
在体外，Phosphatidylglycerols（50μM的剂量分别在0和12小时两次处理）能显著恢复Kdo2-Lipid A (KLA)抑制的RAW264.7细胞总耗氧率（OCR）和线粒体活性[4]。
| Cell experiment [1]: | |
Cell lines | RAW264.7 cells |
Preparation Method | 5 × 104 RAW264.7 cells were seeded into each well of the XF-24 cell culture microplate and allowed to adhere. Then, 50μM Phosphatidylglycerols were added to the cells twice within 12 hours. At 24 hours, 100ng/ml Kdo2-Lipid A (KLA) was added and the cells were incubated for 24 hours. The culture medium was replaced with DMEM at pH 7.4 and the cells were cultured at 37°C for 1 hour. The oxygen consumption rate (OCR) of the cells was analyzed. |
Reaction Conditions | 50μM twice; 12 hours |
Applications | Phosphatidylglycerols significantly rescued the total OCR inhibited by KLA in RAW264.7 cells. |
References: | |
| Cas No. | 383907-64-0 | SDF | |
| 别名 | 磷脂酸甘油酯 | ||
| Canonical SMILES | [R]C(OC[C@@H](OC([R])=O)COP([O-])(OCC(O)CO)=O)=O.[Na+] | ||
| 分子式 | 分子量 | ||
| 溶解度 | Chloroform: 2 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
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