Home>>Signaling Pathways>> Immunology/Inflammation>> Reactive Oxygen Species>>(-)-FINO2

(-)-FINO2

目录号 : GC45248

(-)-FINO2一种能够选择性激活铁死亡的有机过氧化物,用于研究铁死亡机制及抗肿瘤治疗

(-)-FINO2 Chemical Structure

Cas No.:869298-31-7

规格 价格 库存 购买数量
1mg
¥887.00
现货
5mg
¥2,225.00
现货
10mg
¥3,523.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

Description

(-)-FINO2 is an organic peroxide that can selectively induce ferroptosis and is used to study the mechanisms of ferroptosis and anticancer therapy[1]. (-)-FINO2 can specifically induce iron-dependent lipid peroxidation by depleting glutathione and inhibiting GPX4 activity to trigger ferroptosis[2]. (-)-FINO2 can be used to investigate the role of ferroptosis in pathological processes such as tumor suppression, neurodegenerative diseases, and ischemia-reperfusion injury[3-4].

In vitro, treatment of human articular chondrocytes (C-28/I2, T/C-28/A2 cell lines, and primary chondrocytes) with (-)-FINO2 (20μM) for 24 hours significantly reduces cell viability by oxidizing unstable Fe²⁺ and indirectly inhibiting GPX4 activity to trigger ferroptosis[5]. Treatment of HT-1080 fibrosarcoma cells with (-)-FINO2 (10μM) for 24 hours significantly induces cell death via an iron-dependent lipid peroxidation pathway[6].

References:
[1] Abrams RP, Carroll WL, Woerpel KA. Five-Membered Ring Peroxide Selectively Initiates Ferroptosis in Cancer Cells. ACS Chem Biol. 2016 May 20;11(5):1305-12.
[2] Lei Y, Peng X, Hu Y, et al. The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission. Oxid Med Cell Longev. 2020 Dec 3;2020:4132785.
[3] JHuang Y, Ru Q, Ruan H, et al. Changyanning tablet alleviates Crohn's disease by inhibiting GPX4-mediated ferroptosis. J Ethnopharmacol. 2025 Feb 27;342:119415.
[4] Van Kessel ATM, Cosa G. Lipid-derived electrophiles inhibit the function of membrane channels during ferroptosis. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2317616121.
[5] Wipplinger A, Bekric D, Ablinger C, et al. Cannabidiol Is a Potential Inhibitor of Ferroptosis in Human Articular Chondrocytes. J Cell Mol Med. 2025 Jul;29(13):e70592.
[6] Gaschler MM, Andia AA, Liu H, et al. FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515.

(-)-FINO2一种能够选择性激活铁死亡的有机过氧化物,用于研究铁死亡机制及抗肿瘤治疗[1]。(-)-FINO2可以特异性诱导铁依赖性脂质过氧化反应,通过消耗谷胱甘肽和抑制GPX4活性来触发铁死亡[2]。(-)-FINO2 可用于研究铁死亡在肿瘤抑制、神经退行性疾病和缺血再灌注损伤等病理过程中的作用[3-4]

在体外,(-)-FINO2(20μM)处理人关节软骨细胞(C-28/I2、T/C-28/A2细胞系及原代软骨细胞)24小时,(-)-FINO2通过氧化不稳定Fe²⁺并间接抑制GPX4活性触发铁死亡,显著降低细胞活力[5]。(-)-FINO2(10μM)处理HT-1080纤维肉瘤细胞24小时,(-)-FINO2通过铁依赖性脂质过氧化途径显著诱导细胞死亡[6]

实验参考方法

Cell experiment [1]:

Cell lines

HT-1080 fibrosarcoma cells (ferroptosis-sensitive cell line)

Preparation Method

HT-1080 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% CO₂. Cells were treated with 10μM (-)-FINO2 for 6 or 24 hours.

Reaction Conditions

10μM; 6-24 hours

Applications

(-)-FINO2 (10 μM) significantly induces cell death via an iron-dependent lipid peroxidation pathway.

References:
[1] Gaschler MM, Andia AA, Liu H, et al. FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515.

化学性质

Cas No. 869298-31-7 SDF
化学名 (5α,8α)-8-(1,1-dimethylethyl)-3-methyl-1,2-dioxaspiro[4.5]decane-3-ethanol
Canonical SMILES CC(C)(C)[C@@H](CC1)CC[C@]21CC(CCO)(C)OO2
分子式 C15H28O3 分子量 256.4
溶解度 30 mg/ml in DMSO, 30 mg/ml DMF, 30 mg/ml in Ethanol 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 3.9002 mL 19.5008 mL 39.0016 mL
5 mM 780 μL 3.9002 mL 7.8003 mL
10 mM 390 μL 1.9501 mL 3.9002 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置

产品文档

Quality Control & SDS

View current batch: