(-)-FINO2
目录号 : GC45248(-)-FINO2一种能够选择性激活铁死亡的有机过氧化物，用于研究铁死亡机制及抗肿瘤治疗
Cas No.:869298-31-7
Sample solution is provided at 25 µL, 10mM.
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(-)-FINO2 is an organic peroxide that can selectively induce ferroptosis and is used to study the mechanisms of ferroptosis and anticancer therapy[1]. (-)-FINO2 can specifically induce iron-dependent lipid peroxidation by depleting glutathione and inhibiting GPX4 activity to trigger ferroptosis[2]. (-)-FINO2 can be used to investigate the role of ferroptosis in pathological processes such as tumor suppression, neurodegenerative diseases, and ischemia-reperfusion injury[3-4].
In vitro, treatment of human articular chondrocytes (C-28/I2, T/C-28/A2 cell lines, and primary chondrocytes) with (-)-FINO2 (20μM) for 24 hours significantly reduces cell viability by oxidizing unstable Fe²⁺ and indirectly inhibiting GPX4 activity to trigger ferroptosis[5]. Treatment of HT-1080 fibrosarcoma cells with (-)-FINO2 (10μM) for 24 hours significantly induces cell death via an iron-dependent lipid peroxidation pathway[6].
References:
[1] Abrams RP, Carroll WL, Woerpel KA. Five-Membered Ring Peroxide Selectively Initiates Ferroptosis in Cancer Cells. ACS Chem Biol. 2016 May 20;11(5):1305-12.
[2] Lei Y, Peng X, Hu Y, et al. The Calcilytic Drug Calhex-231 Ameliorates Vascular Hyporesponsiveness in Traumatic Hemorrhagic Shock by Inhibiting Oxidative Stress and miR-208a-Mediated Mitochondrial Fission. Oxid Med Cell Longev. 2020 Dec 3;2020:4132785.
[3] JHuang Y, Ru Q, Ruan H, et al. Changyanning tablet alleviates Crohn's disease by inhibiting GPX4-mediated ferroptosis. J Ethnopharmacol. 2025 Feb 27;342:119415.
[4] Van Kessel ATM, Cosa G. Lipid-derived electrophiles inhibit the function of membrane channels during ferroptosis. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2317616121.
[5] Wipplinger A, Bekric D, Ablinger C, et al. Cannabidiol Is a Potential Inhibitor of Ferroptosis in Human Articular Chondrocytes. J Cell Mol Med. 2025 Jul;29(13):e70592.
[6] Gaschler MM, Andia AA, Liu H, et al. FINO2 initiates ferroptosis through GPX4 inactivation and iron oxidation. Nat Chem Biol. 2018 May;14(5):507-515.
(-)-FINO2一种能够选择性激活铁死亡的有机过氧化物，用于研究铁死亡机制及抗肿瘤治疗[1]。(-)-FINO2可以特异性诱导铁依赖性脂质过氧化反应，通过消耗谷胱甘肽和抑制GPX4活性来触发铁死亡[2]。(-)-FINO2 可用于研究铁死亡在肿瘤抑制、神经退行性疾病和缺血再灌注损伤等病理过程中的作用[3-4]。
在体外，(-)-FINO2（20μM）处理人关节软骨细胞（C-28/I2、T/C-28/A2细胞系及原代软骨细胞）24小时，(-)-FINO2通过氧化不稳定Fe²⁺并间接抑制GPX4活性触发铁死亡，显著降低细胞活力[5]。(-)-FINO2（10μM）处理HT-1080纤维肉瘤细胞24小时，(-)-FINO2通过铁依赖性脂质过氧化途径显著诱导细胞死亡[6]。
| Cell experiment [1]: | |
Cell lines | HT-1080 fibrosarcoma cells (ferroptosis-sensitive cell line) |
Preparation Method | HT-1080 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% CO₂. Cells were treated with 10μM (-)-FINO2 for 6 or 24 hours. |
Reaction Conditions | 10μM; 6-24 hours |
Applications | (-)-FINO2 (10 μM) significantly induces cell death via an iron-dependent lipid peroxidation pathway. |
References: | |
| Cas No. | 869298-31-7 | SDF | |
| 化学名 | (5α,8α)-8-(1,1-dimethylethyl)-3-methyl-1,2-dioxaspiro[4.5]decane-3-ethanol | ||
| Canonical SMILES | CC(C)(C)[C@@H](CC1)CC[C@]21CC(CCO)(C)OO2 | ||
| 分子式 | C15H28O3 | 分子量 | 256.4 |
| 溶解度 | 30 mg/ml in DMSO, 30 mg/ml DMF, 30 mg/ml in Ethanol | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9002 mL | 19.5008 mL | 39.0016 mL |
| 5 mM | 780 μL | 3.9002 mL | 7.8003 mL |
| 10 mM | 390 μL | 1.9501 mL | 3.9002 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet