H-Val-Pro-Pro-OH (trifluoroactetate salt)
(Synonyms: VPP) 目录号 : GC47438
A neuropeptide with diverse biological activities
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
H-Val-Pro-Pro-OH (VPP) is a peptide inhibitor of angiotensin-converting enzyme (ACE; IC50 = 9 µM).1 It inhibits acetylcholine-induced nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs) when used at a concentration of 1 µM and induces vasorelaxation in precontracted isolated rat aortic rings.2 VPP (0.3 mg/kg) decreases systolic blood pressure in spontaneously hypertensive, but not normotensive, rats.3 Dietary administration of VPP reduces plasma levels of total cholesterol, HDL-cholesterol, and triglycerides, expression of Il6 and Il1b, and aortic arch intimal thickening and atherosclerotic plaque formation in ApoE-/- mice.4
1.Nakamura, Y., Yamamoto, N., Sakai, K., et al.Purification and characterization of angiotensin I-converting enzyme inhibitors from sour milkJ. Dairy Sci.78(4)777-783(1995) 2.Hirota, T., Nonaka, A., Matsushita, A., et al.Milk casein-derived tripeptides, VPP and IPP induced NO production in cultured endothelial cells and endothelium-dependent relaxation of isolated aortic ringsHeart Vessels26(5)549-556(2011) 3.Nakamura, Y., Yamamoto, N., Sakai, K., et al.Antihypertensive effect of sour milk and peptides isolated from it that are inhibitors to angiotensin I-converting enzymeJ. Dairy Sci.78(6)1253-1257(1995) 4.Nakamura, T., Hirota, T., Mizushima, K., et al.Milk-derived peptides, Val-Pro-Pro and Ile-Pro-Pro, attenuate atherosclerosis development in apolipoprotein e-deficient mice: A preliminary studyJ. Med. Food16(5)396-403(2013)
| Cas No. | N/A | SDF | |
| 别名 | VPP | ||
| Canonical SMILES | CC(C)[C@H](N)C(N1CCC[C@H]1C(N2CCC[C@H]2C(O)=O)=O)=O.OC(C(F)(F)F)=O | ||
| 分子式 | C15H25N3O4.XCF3COOH | 分子量 | 311.4 |
| 溶解度 | DMF: 15mg/mL,DMSO: 15mg/mL,Ethanol: 15mg/mL,PBS (pH 7.2): 10mg/mL | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.2113 mL | 16.0565 mL | 32.113 mL |
| 5 mM | 0.6423 mL | 3.2113 mL | 6.4226 mL |
| 10 mM | 0.3211 mL | 1.6057 mL | 3.2113 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。