Fuscin
(Synonyms: 暗褐菌素) 目录号 : GC40008
A fungal metabolite with diverse biological activities
Cas No.:83-85-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fuscin is a quinonoid fungal metabolite originally isolated from O. fuscum that has diverse biological activities. It inhibits binding of the ADP/ATP translocase inhibitor atractyloside to rat liver mitochondria in an ADP-dependent manner when used at a concentration of 50 μM in a radioligand binding assay. Fuscin (20 μM) reduces the glutathione content of rat liver mitochondria to 28% of controls and inhibits NADH oxidation in sonicated pigeon heart mitochondria preparations in a concentration-dependent manner. It competes with macrophage inflammatory protein 1α (MIP-1α) for binding to CCR5 chemokine receptors in vitro with an IC50 value of 21 μM.
| Cas No. | 83-85-2 | SDF | |
| 别名 | 暗褐菌素 | ||
| Canonical SMILES | O=C1OC(C)C(C2=C1)=C(O)C(C3=C2CCC(C)(C)O3)=O | ||
| 分子式 | C15H16O5 | 分子量 | 276.3 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6193 mL | 18.0963 mL | 36.1925 mL |
| 5 mM | 0.7239 mL | 3.6193 mL | 7.2385 mL |
| 10 mM | 0.3619 mL | 1.8096 mL | 3.6193 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
10-Methoxydihydrofuscin, fuscinarin, and Fuscin, novel antagonists of the human CCR5 receptor from Oidiodendron griseum
J Nat Prod 2003 Aug;66(8):1116-7.PMID:12932138DOI:10.1021/np030146m.
Two new compounds, 10-methoxydihydrofuscin (1) and fuscinarin (2), and one known compound, Fuscin (3), have been isolated from the soil fungus Oidiodendron griseum. These compounds were found to compete effectively with macrophage inflammatory protein (MIP)-1 alpha for binding to human CCR5, an important anti HIV-1 target that interferes with HIV entry into cells. The structures of these compounds were elucidated by spectroscopic methods.
Elemental analysis of frog outer segment and Fuscin granule by means of x-ray microanalyzer
Sens Processes 1978 Dec;2(4):285-95.PMID:755284doi
Elemental analysis was made on the rod outer segments and Fuscin granules in the frog eye by means of an energy-dispersive X-ray spectrometer combined with an electron microscope. The materials were prepared from the unfixed fresh retina by means of air-dried or freeze-dried cryosection, freeze-substitution, or the freeze-drying embedding method. An outer segment in the air-dried cryosection showed P, K, S, Cl, and Ca peaks. The dry section of freeze-substituted or freeze-dried embedded outer segment showed P, K, S, and Cl; K was lost in the wet section. The Fuscin granules showed prominent peaks of Ca and Zn constantly, in addition to S, Cl, Mg, K, and occasionally Cu. The K peak was also lost in the wet section.
Bioactive Metabolites from the Deep Subseafloor Fungus Oidiodendron griseum UBOCC-A-114129
Mar Drugs 2017 Apr 7;15(4):111.PMID:28387732DOI:10.3390/md15040111.
Four bioactive compounds have been isolated from the fungus Oidiodendron griseum UBOCC-A-114129 cultivated from deep subsurface sediment. They were structurally characterized using a combination of LC-MS/MS and NMR analyses as Fuscin and its derivatives (dihydrofuscin, dihydrosecofuscin, and secofuscin) and identified as polyketides. Albeit those compounds were already obtained from terrestrial fungi, this is the first report of their production by an Oidiodendron species and by the deepest subseafloor isolate ever studied for biological activities. We report a weak antibacterial activity of dihydrosecofuscin and secofuscin mainly directed against Gram-positive bacteria (Minimum Inhibitory Concentration (MIC) equal to Minimum Bactericidal Concentration (MBC), in the range of 100 μg/mL). The activity on various protein kinases was also analyzed and revealed a significant inhibition of CDC2-like kinase-1 (CLK1) by dihysecofuscin.
Root canal morphology of the mandibular first premolars in an Iranian population using cross-sections and radiography
J Endod 2010 Feb;36(2):214-7.PMID:20113777DOI:10.1016/j.joen.2009.10.002.
Introduction: Successful endodontic treatment is dependent on a perfect knowledge of normal root canal anatomy and variations from the norm. This study was aimed to investigate the root canal anatomy of mandibular first premolars by both radiography and cross-sectional methods in an Iranian population. Methods: Two hundred seventeen extracted human mandibular first premolars were used. Radiographs were taken in both mesiodistal and buccolingual directions for each premolar. Then, all the teeth were decoronated at the cementoenamel junction and resected perpendicular to their long axes into three equal sections and stained with Fuscin. Digital photographs of the cross-sectional root surfaces were taken and surveyed at 40x magnification. The root canal configuration types were determined separately by radiographs and cross-sectional images for each premolar. Results: Out of 217 teeth examined, 192 (88.47%) had a single root canal. The remaining 25 teeth (11.53%) showed two canals in at least one cross-section of their roots with five root canal configurations. In the mesiodistal (MD) radiographs, only 5.99% of premolars showed two canals with three root canal configurations. Conclusions: One-hundred ninety two out of 217 premolars showed one canal in both cross-section and MD radiography. Only 7 premolars were diagnosed as having more than one canal with the same canal configuration in both cross-sections and MD radiography methods.
Studies on the endoplasmic reticulum. V. Its form and differentiation in pigment epithelial cells of the frog retina
J Biophys Biochem Cytol 1960 Sep;8(1):181-205.PMID:13737277DOI:10.1083/jcb.8.1.181.
Pigment epithelial cells of the frog's retina have been examined by methods of electron microscopy with special attention focused on the fine structure of the endoplasmic reticulum and the myeloid bodies. These cells, as reported previously, send apical prolongations into the spaces between the rod outer segments, and within these extensions, pigment migrates in response to light stimulation. The cytoplasm of these cells is filled with a compact lattice of membrane-limited tubules, the surfaces of which are smooth or particle-free. In this respect, the endoplasmic reticulum here resembles that encountered in cells which produce lipid-rich secretions. The myeloid bodies comprise paired membranes arranged in stacks shaped like biconvex lenses. At their margins the membranes are continuous with elements of the ER and in consequence of this the myeloid body is referred to as a differentiation of the reticulum. The paired membranes resemble in their thickness and spacings those which make up the outer segments; they are therefore regarded as intracellular photoreceptors of possible significance in the activation of pigment migration and other physiologic functions of these cells. The Fuscin granules are enclosed in membranes which are also continuous with those of the ER. The granules seem to move independently of the prolongations in which they are contained. The report also describes the fine structure of the terminal bar apparatus, the fibrous layer intervening between the epithelium and the choroid blood vessels, and comments on the functions of the organelles depicted.