Desethyl Hydroxychloroquine
(Synonyms: 克尼托喹,Cletoquine?) 目录号 : GC47192An active metabolite of hydroxychloroquine
Cas No.:4298-15-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
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Desethyl hydroxychloroquine is an active metabolite of hydroxychloroquine .1 It is formed via the desethylation of hydroxychloroquine in the liver, a process mediated by the cytochrome P450 (CYP) isoforms CYP2D6, CYP3A4, CYP3A5, and CYP2C8.
1.Charlier, B., Pingeon, M., Dal Piaz, F., et al.Development of a novel ion-pairing HPLC-FL method for the separation and quantification of hydroxychloroquine and its metabolites in whole bloodBiomed. Chromatogr.32(8)e4258(2018)
Cas No. | 4298-15-1 | SDF | |
别名 | 克尼托喹,Cletoquine? | ||
Canonical SMILES | ClC1=CC2=C(C=C1)C(NC(C)CCCNCCO)=CC=N2 | ||
分子式 | C16H22ClN3O | 分子量 | 307.8 |
溶解度 | DMF: soluble,DMSO: soluble,Methanol: soluble | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 3.2489 mL | 16.2443 mL | 32.4886 mL |
5 mM | 0.6498 mL | 3.2489 mL | 6.4977 mL |
10 mM | 0.3249 mL | 1.6244 mL | 3.2489 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
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% DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
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A Capillary Electrophoresis-Based Method for the Measurement of Hydroxychloroquine and Its Active Metabolite Desethyl Hydroxychloroquine in Whole Blood in Patients with Rheumatoid Arthritis
Molecules 2022 Jun 17;27(12):3901.PMID:35745021DOI:10.3390/molecules27123901.
A capillary electrophoresis method was developed to detect and measure hydroxychloroquine (HCQ) and its active metabolite Desethyl Hydroxychloroquine (DHCQ) in whole blood in patients with rheumatoid arthritis. The best separation in terms of peak area reproducibility, migration time, peak shape, and resolution of adjacent peaks was obtained in a 60 cm, 75 µm i.d. uncoated fused-silica capillary using a background electrolyte mixture of an aqueous 55 mmol/L TRIS solution brought to pH 2.6 with phosphoric acid and methanol (85:15) and a voltage and a temperature of separation of 20 kV and 30 °C, respectively. Analytes were separated in less than 12 min, with excellent linearity (R2 ≥ 0.999) in the concentration range of 0.5-8 µmol/L. The recovery of analytes spiked in whole blood was 99-101% for HCQ and 98-99% for DHCQ. Analysis of five samples from patients with rheumatoid arthritis receiving HCQ 400 mg daily yielded mean steady-state concentrations of 2.27 ± 1.61 and 1.54 ± 0.55 μmol/L for HCQ and DHCQ, respectively, with a HCQ to DHCQ ratio of 1.40 ± 0.77.