Cobaltic Protoporphyrin IX (chloride)
(Synonyms: 原卟啉氯化钴) 目录号 : GC49096
Cobaltic Protoporphyrin IX (chloride)是一种高价钴配位卟啉化合物。Cobaltic Protoporphyrin IX (chloride)通过上调HO-1的抗氧化、抗炎和细胞保护功能，有助于减轻免疫相关的肾脏损伤。
Cas No.:102601-60-5
Sample solution is provided at 25 µL, 10mM.
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Cobaltic Protoporphyrin IX (chloride) is a high-valent cobalt-coordinated porphyrin compound [1]. Cobaltic Protoporphyrin IX (chloride) helps alleviate immune-related renal injury by upregulating the antioxidant, anti-inflammatory and cytoprotective functions of HO-1 [2]. Cobaltic Protoporphyrin IX (chloride) is often used to mimic the functions of heme-dependent enzymes such as cytochromes enzymes [3].
In human microvascular endothelial cells, HO-1 was significantly upregulated at both mRNA and protein levels in cells treated with Cobaltic Protoporphyrin IX (chloride) (10μM; 24h) [4]. In muscle-derived stem cells, a single exposure to Cobaltic Protoporphyrin IX (chloride) (25μM; 24-96h) increased HO-1 protein expression and enzyme activity [5]. In HCT116, HT29 and DLD-1 cells, Cobaltic Protoporphyrin IX (chloride) (30μM; 48h) treatment directly hindered the binding between HSP90 and HIF-1α [6]. In HaCaT cells, Cobaltic Protoporphyrin IX (chloride) (10μM; 5d) treatment was found to enhance cell migration in high glucose media [7].
In HO-1−/− mice, Cobaltic Protoporphyrin IX (chloride) (10mg/kg; ip; 5d) increases endogenous G‐CSF and mobilizes HSC and granulocytes to the blood [8]. In male Wistar rats, Cobaltic Protoporphyrin IX (chloride) (125μmol/kg; sc; 5d) profoundly decreased the levels of hepatic microsomal heme [9]. In C57BL/6J mice, the highest G-CSF levels and numbers of mobilized leukocytes were observed in Cobaltic Protoporphyrin IX (chloride) (10mg/kg; ip; 5d)-treated mice [10].
References:
[1]. Watkins S, Baron J, Tephly TR. Identification of cobalt protoporphyrin IX formation in vivo following cobalt administration to rats. Biochemical Pharmacology. 1980 Sep 1; 29(17): 2319-2323.
[2]. Lianos EA, Phung GN, Zhou J, et al. Cobalt Protoporphyrin IX Attenuates Antibody-Mediated, Complement-Dependent Podocyte Injury: Role of Cobalt and Porphyrin Moieties. Inorganics. 2025 Feb 23; 13(3): 66.
[3]. Tomaro ML, Frydman J, Frydman RB. Heme oxygenase induction by CoCl2, Co-protoporphyrin IX, phenylhydrazine, and diamide: evidence for oxidative stress involvement. Archives of Biochemistry and Biophysics. 1991 May 1; 286(2): 610-617.
[4]. Loboda A, Jazwa A, Wegiel B, et al. Heme oxygenase-1-dependent and-independent regulation of angiogenic genes expression: effect of cobalt protoporphyrin and cobalt chloride on VEGF and IL-8 synthesis in human microvascular endothelial cells. Cellular and molecular biology (Noisy-le-Grand, France). 2005 Sep 30; 51(4): 347.
[5]. Wilson HM, Welikson RE, Luo J, et al. Can cytoprotective cobalt protoporphyrin protect skeletal muscle and muscle-derived stem cells from ischemic injury?. Clinical Orthopaedics and Related Research®. 2015 Sep; 473: 2908-2919.
[6]. Lee WH, Lee JM, Lim C, et al. Structural requirements within protoporphyrin IX in the inhibition of heat shock protein 90. Chemico-biological interactions. 2013 Jun 25; 204(1): 49-57.
[7]. Fang PH, Lai YY, Chen CL, et al. Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions. Molecular Medicine. 2022 Dec; 28(1): 71.
[8]. Szade A, Szade K, Nowak WN, et al. Cobalt protoporphyrin IX increases endogenous G‐CSF and mobilizes HSC and granulocytes to the blood. EMBO Molecular Medicine. 2019 Dec; 11(12): e09571.
[9]. Cheeseman KH, Albano EF, Tomasi A, et al. The effect of the administration of cobaltic protoporphyrin IX on drug metabolism, carbon tetrachloride activation and lipid peroxidation in rat liver microsomes. Chemico-Biological Interactions. 1984 Jul 1; 50(2): 143-151.
[10]. Bednarz A, Kozuch P, Kowalski K, et al. Dose-and time-dependent effects of cobalt protoporphyrin IX on granulocyte mobilization and metabolic markers in mice. bioRxiv. 2024 Oct 25:2024-10.
Cobaltic Protoporphyrin IX (chloride)是一种高价钴配位卟啉化合物 [1]。Cobaltic Protoporphyrin IX (chloride)通过上调HO-1的抗氧化、抗炎和细胞保护功能，有助于减轻免疫相关的肾脏损伤 [2]。Cobaltic Protoporphyrin IX (chloride)常用于模拟血红素依赖性酶（如细胞色素）的功能 [3]。在人微血管内皮细胞中，经Cobaltic Protoporphyrin IX (chloride)（10μM；24h）处理的细胞中，HO-1在mRNA和蛋白质水平上均显著上调 [4]。在肌肉来源的干细胞中，单次暴露于Cobaltic Protoporphyrin IX (chloride)（25μM；24-96h）可增加HO-1蛋白表达和酶活性 [5]。在HCT116、HT29和DLD-1细胞中，Cobaltic Protoporphyrin IX (chloride)（30μM；48h）处理直接抑制了HSP90与HIF-1α的结合 [6]。在HaCaT细胞中，Cobaltic Protoporphyrin IX (chloride)（10μM；5d）处理可增强高糖培养基中的细胞迁移 [7]。在HO-1-/-小鼠中，Cobaltic Protoporphyrin IX (chloride)（10mg/kg；ip；5d）可增加内源性G-CSF，并动员造血干细胞（HSC）和粒细胞入血 [8]。在雄性Wistar大鼠中，Cobaltic Protoporphyrin IX (chloride)（125μmol/kg；sc；5d）显著降低了肝微粒体血红素水平 [9]。在C57BL/6J小鼠中，Cobaltic Protoporphyrin IX (chloride)（10mg/kg；ip；5d）治疗的小鼠的G-CSF水平和动员白细胞数量最高 [10]。
| Cell experiment [1]: | |
Cell lines | Human microvascular endothelial cells (HMEC-1) |
Preparation Method | Cells were seeded in 24-well or 6-well plates and grown to full confluence. Next, medium was changed and stimulants were added for 6 or 24h. After this time media were collected for determining VEGF and IL-8 protein concentration, while cells were washed twice with cold PBS and then subjected to RNA or protein isolations for RT-PCR analysis or Western blotting, respectively. Cobaltic Protoporphyrin IX (chloride), SnPPIX and CoCl2 were prepared freshly and added to cells for indicated time. SnPPIX and N-acetylcysteine were applied 1h before stimulation. Solvent (0.1M NaOH for Cobaltic Protoporphyrin IX (chloride) and SnPPIX) at appropriate final dilution was included in control. |
Reaction Conditions | 10μM; 24h |
Applications | HO-1 was significantly upregulated at both mRNA and protein levels in cells treated with Cobaltic Protoporphyrin IX (chloride). |
| Animal experiment [2]: | |
Animal models | HO-1−/− mice |
Preparation Method | Cobaltic Protoporphyrin IX (chloride) and SnPP were dissolved in DMSO at concentration of 0.2g/mL. The stock solutions were diluted 160× in 0.9% NaCl solution. Mice were injected intraperitoneally (i.p.) at the dose of 10mg/kg (15μmol/kg). Recombinant human G‐CSF (rhG‐CSF; Amgen) was used at the dose of 250μg/kg. Murine recombinant IL‐6 (R&D) was used in concentration 50μg/kg. |
Dosage form | 10mg/kg; ip; 5d |
Applications | Cobaltic Protoporphyrin IX (chloride) increases endogenous G‐CSF and mobilizes HSC and granulocytes to the blood. |
References: | |
| Cas No. | 102601-60-5 | SDF | |
| 别名 | 原卟啉氯化钴 | ||
| Canonical SMILES | [Cl-][Co+3]1([N](C2=C3)=C(C(C=C)=C2C)C=C4C(C)=C5CCC([O-])=O)([N-]6C3=C7C=C)[N-]4C5=CC(C(CCC([O-])=O)=C8C)=[N]1C8=CC6=C7C.[H+].[H+] | ||
| 分子式 | C34H30ClCoN4O4·2H | 分子量 | 655 |
| 溶解度 | DMF: 30 mg/ml,DMF:PBS (pH 7.2) (1:6): 0.14 mg/ml,DMSO: 25 mg/ml,Ethanol: 1 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5267 mL | 7.6336 mL | 15.2672 mL |
| 5 mM | 0.3053 mL | 1.5267 mL | 3.0534 mL |
| 10 mM | 0.1527 mL | 0.7634 mL | 1.5267 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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