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Bafilomycin A1 目录号 GC17597

V-ATPase inhibitor,selective and reversible

规格 价格 库存 购买数量
500µg
¥1,071.00
现货
1mg
¥2,041.00
现货
5mg
¥8,568.00
现货

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Sample solution is provided at 25 µL, 10mM.

质量管理

Quality Control & SDS

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实验参考方法

Cell experiment [1]:

Cell lines

HeLa cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 20 nM

Applications

Bafilomycin A1 dose-dependently inhibited the vacuolization of Hela cells induced by H. pylori, showing a 50% effect at 4 nM and a complete inhibition at 12.5 nM. In addition, Bafilomycin A1 also efficiently restored vacuolated cells to a normal appearance.

Animal experiment [2]:

Animal models

Young freshwater tilapias

Dosage form

0 ~ 10-5 mol/L; 30 mins

Applications

In young tilapias, Bafilomycin A1 dose-dependently inhibited the rate of Na+ uptake with a Ki value of 1.6 × 10-7 mol/L. The inhibitory effect (20%) was observed at a concentration as low as 10-8 mol/L and increased linearly up to a concentration of 10-6 mol/L, after which it remained at approximately 90 % inhibition.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Papini E, Bugnoli M, De Bernard M, Figura N, Rappuoli R, Montecucco C. Bafilomycin A1 inhibits Helicobacter pylori-induced vacuolization of HeLa cells. Mol Microbiol. 1993 Jan;7(2):323-7.

[2]. Fenwick JC, Wendelaar Bonga SE, Flik G. In vivo bafilomycin-sensitive Na(+) uptake in young freshwater fish. J Exp Biol. 1999 Dec;202 Pt 24:3659-66.

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Chemical Properties

Cas No. 88899-55-2 SDF
别名 NSC 381866
化学名 (3Z,5E,7R,8S,9S,11E,13E,15S,16R)-16-[(2S,3R,4S)-4-[(2R,4R,5S,6R)-2,4-dihydroxy-5-methyl-6-propan-2-yloxan-2-yl]-3-hydroxypentan-2-yl]-8-hydroxy-3,15-dimethoxy-5,7,9,11-tetramethyl-1-oxacyclohexadeca-3,5,11,13-tetraen-2-one
Canonical SMILES CC1CC(=CC=CC(C(OC(=O)C(=CC(=CC(C1O)C)C)OC)C(C)C(C(C)C2(CC(C(C(O2)C(C)C)C)O)O)O)OC)C
分子式 C35H58O9 分子量 622.84
溶解度 5 mg/ml in DMSO or in menthanol 储存条件 Desiccate at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

产品描述

Bafilomycin A1 is a selective, reversible inhibitor of vacuolar H+-ATPases (V-ATPases), blocking these proton pumps in mammalian, plant, or fungal cells with an IC50 value in the 4-400 nM range.[1] It is at least 1,000-fold less potent at most other types of ATPases.[1] Bafilomycin A1 also inhibits autophagy by preventing vacuolar acidification necessary for autophagosome maturation.[2],[3]

Reference:
[1]. Bowman, E.J., Siebers, A., and Altendorf, K. Bafilomycins: A class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proceedings of the National Academy of Sciences of the United States of America 85, 7972-7976 (1988).
[2]. Yamamoto, A., Tagawa, Y., Yoshimori, T., et al. Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Structure and Function 23, 33-42 (1998).
[3]. Shacka, J.J., Klocke, B.J., and Roth, K.A. Autophagy, bafilomycin and cell death: The "A-B-Cs" of plecomacrolide-induced neuroprotection. Autophagy 2(3), 228-230 (2006).