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T4 DNA

目录号 : GP22276

T4 DNA Ligase Recombinant

T4 DNA Chemical Structure

规格 价格 库存 购买数量
20,000IU
¥910.00
5-10工作日
100,000IU
¥3,570.00
5-10工作日
500,000IU
¥13,860.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

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产品描述

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

Product Data

Purity Source Escherichia Colilambda lysogen NM 989.
Phycical Appearance Sterile filtered liquid formulation having a concentration of 167,000 U/ml. Shipping Condition Shipped with Ice Packs.
Synonyms DNA ligase 4; EC 6.5.1.1; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4.
Biological Activity One Weiss unit is equivalent to circa 67 cohesive-end ligation units.? T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.? Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50?M.? To dilute T4 DNA Ligase that will subsequently be stored at иC20°C , 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.
Formulation 50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 ?m in 5? termini). Optimal ligation occurs at 16C.

Biological Activity

One Weiss unit is equivalent to circa 67 cohesive-end ligation units.? T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.? Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50?M.? To dilute T4 DNA Ligase that will subsequently be stored at иC20°C , 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.

Applications

Cloning of restriction fragments.Joining linkers and adapters to blunt-ended DNA.