Suberohydroxamic Acid
(Synonyms: 软木肟酸,Suberohydroxamic acid; SBHA) 目录号 : GC12976
HDAC inhibitor
Cas No.:38937-66-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Suberohydroxamic Acid (Suberoyl bis-hydroxamic acid, SBHA) is an inhibitor of HDAC with ID50 values of 0.25 and 0.30 μM for HDAC1 and HDAC3, respectively [1].
Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from ε-N-acetyl lysines on histones, allowing the histones to wrap the DNA more tightly. DNA expression is regulated by de-acetylation and acetylation.
Suberohydroxamic Acid (SBHA) is an HDAC inhibitor. In MEL cells, SBHA induced the accumulation of acetylated H4 [1]. In medullary thyroid carcinoma (MTC) cells, SBHA dose-dependently induced the Notch-1 intracellular domain (the active form of Notch-1), which then decreased NE tumor marker chromogranin A (CgA) and achaete-scute complex-like 1 (ASCL-1), a downstream target of Notch-1 signaling. Also, SBHA increased the levels of cleaved poly ADP-ribose polymerase (PARP) and caspase-3, induced apoptosis and reduced cell viability in a dose-dependent way [2]. In MCF-7 breast cancer cells, SBHA induced the expressions of p53, p21, Bax, and PUMA, and ΔΨm collapsed, which then induced apoptosis [3]. In acute T lymphoblastic leukemia (T-ALL) cells, SBHA enhanced WNT/β-catenin signaling, blocked G2/M cell cycle progression, increased p21(WAF1) expression and inhibited cell growth. SBHA also increased the levels of cleaved PARP, caspase-9 and caspase-3, and induced apoptosis [4].
References:
[1]. Richon VM, Emiliani S, Verdin E, et al. A class of hybrid polar inducers of transformed cell differentiation inhibits histone deacetylases. Proc Natl Acad Sci U S A, 1998, 95(6): 3003-3007.
[2]. Ning L, Greenblatt DY, Kunnimalaiyaan M, et al. Suberoyl bis-hydroxamic acid activates Notch-1 signaling and induces apoptosis in medullary thyroid carcinoma cells. Oncologist, 2008, 13(2): 98-104.
[3]. Zhuang ZG, Fei F, Chen Y, et al. Suberoyl bis-hydroxamic acid induces p53-dependent apoptosis of MCF-7 breast cancer cells. Acta Pharmacol Sin, 2008, 29(12): 1459-1466.
[4]. Shao N, Zou J, Li J, et al. Hyper-activation of WNT/β-catenin signaling pathway mediates anti-tumor effects of histone deacetylase inhibitors in acute T lymphoblastic leukemia. Leuk Lymphoma, 2012, 53(9): 1769-1778.
| Cas No. | 38937-66-5 | SDF | |
| 别名 | 软木肟酸,Suberohydroxamic acid; SBHA | ||
| 化学名 | N1,N8-dihydroxyoctanediamide | ||
| Canonical SMILES | ON([H])C(CCCCCCC(N(O)[H])=O)=O | ||
| 分子式 | C8H16N2O4 | 分子量 | 204.22 |
| 溶解度 | >9.2mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.8967 mL | 24.4834 mL | 48.9668 mL |
| 5 mM | 0.9793 mL | 4.8967 mL | 9.7934 mL |
| 10 mM | 0.4897 mL | 2.4483 mL | 4.8967 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。