sCD23 Human
目录号 : GP23021Soluble CD23 Human Recombinant
Sample solution is provided at 25 µL, 10mM.
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
Synonyms | Low affinity immunoglobulin epsilon Fc receptor; Lymphocyte IgE receptor; Fc-epsilon-RII; BLAST-2; Immunoglobulin E-binding factor; CD23 antigen; FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J. | ||
Amino Acid Sequence | MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS | ||
Solubility | It is recommended to reconstitute the lyophilized sCD23 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
Stability | Lyophilized sCD23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution sCD23 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. | ||
Biological Activity | The ED50 is determined by its ability to induce TNF-alpha production by human PBMCs. | ||
Formulation | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
CD23 is a 45kDa glycoprotein, which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma.
The ED50 is determined by its ability to induce TNF-alpha production by human PBMCs.
Lyophilized sCD23 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution sCD23 should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.