RNASE3 Human
目录号 : GP22221Ribonuclease 3 Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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RNASE3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (28-160) and having a molecular mass of 19.9kDa. RNASE3 is fused to a 38 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| Purity | Greater than 90.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | ECP; RNS3; Ribonuclease 3; Eosinophil cationic protein; RNASE3; RNASE3. | ||
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRP PQFTRAQWFA IQHISLNPPR CTIAMRAINN YRWRCKNQNT FLRTTFANVV NVCGNQSIRC PHNRTLNNCH RSRFRVPLLH CDLINPGAQN ISNCRYADRP GRRFYVVACD NRDPRDSPRY PVVPVHLDTT I. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | The RNASE3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. | ||
Ribonuclease 3 (RNASE3) is cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. RNASE3 possesses a broad diversity of biological activities. RNASE3 protein has shown antibacterial activity such as cytoplasmic membrane depolarization of preferentially Gram-negative and Gram-positive strains and promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.