trans-Zeatin
(Synonyms: 反-玉米素) 目录号 : GC30162
trans-Zeatin是一种天然的细胞分裂素（cytokinin），属于植物生长调节剂，在植物细胞生长、分化和分裂中起核心作用。
Cas No.:1637-39-4
Sample solution is provided at 25 µL, 10mM.
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trans-Zeatin is a natural cytokinin, a plant growth regulator that plays a central role in plant cell growth, differentiation, and division[1, 2]. trans-Zeatin can be used as a culture medium additive to induce plant callus growth and bud formation[3]. trans-Zeatin is a potential therapeutic agent for skin photoaging[4].
In vitro, treatment of human skin fibroblasts (HSFs) with trans-Zeatin (20-80μM) for 24h enhanced the cell viability of UVB-irradiated HSFs and significantly inhibited UVB-induced matrix metalloproteinase-1 (MMP-1) expression[5]. Treatment of skin keratinocytes (HaCaT cells) with trans-Zeatin (20-80μM) for 4-24h upregulated the expression of aquaporin-3 (AQP3) in HaCaT cells in a time- and dose-dependent manner[6].
References:
[1] Schäfer M, Brütting C, Meza-Canales I D, et al. The role of cis-zeatin-type cytokinins in plant growth regulation and mediating responses to environmental interactions[J]. Journal of experimental botany, 2015, 66(16): 4873-4884.
[2] Großkinsky D, Edelsbrunner K, Pfeifhofer H, et al. Cis-and trans-zeatin differentially modulate plant immunity[J]. Plant signaling & behavior, 2013, 8(7): e24798.
[3] Cardoza V. Tissue culture: The manipulation of plant development[J]. Plant Biotechnology and Genetics: principles, techniques, and applications, 2008: 113-134.
[4] Kesharwani A, Panda S P. Isolation of Phytohormone trans-Zeatin: Potential Oxidant Scavenger and Anti-Aging Compound[J]. Neurochemical Journal, 2024, 18(1): 134-146.
[5] Yang B, Ji C, Kang J, et al. Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts[J]. International Journal of Molecular Medicine, 2009, 23(4): 555-560.
[6] Ji C, Yang Y, Yang B, et al. Trans-Zeatin attenuates ultraviolet induced down-regulation of aquaporin-3 in cultured human skin keratinocytes[J]. International journal of molecular medicine, 2010, 26(2): 257-263.
trans-Zeatin是一种天然的细胞分裂素（cytokinin），属于植物生长调节剂，在植物细胞生长、分化和分裂中起核心作用[1, 2]。trans-Zeatin能够用作培养基的添加剂，用于诱导植物愈伤组织生长和芽的形成[3]。trans-Zeatin是一种潜在的皮肤光老化治疗药物[4]。
在体外，trans-Zeatin（20-80μM）处理人皮肤成纤维细胞（HSFs）24h，增强了接受UVB照射的HSFs的细胞活力，显著抑制了UVB诱导的基质金属蛋白酶-1（MMP-1）表达[5]。trans-Zeatin（20-80μM）处理皮肤角质形成细胞（HaCaT 细胞）4-24h，以时间和剂量依赖性的方式上调了HaCaT细胞中的水通道蛋白-3（AQP3）的表达[6]。
| Cell experiment [1]: | |
Cell lines | Human skin fibroblasts (HSFs) |
Preparation Method | Human skin fibroblasts (HSFs) were seeded in a 96-well plate. After various concentrations of trans-Zeatin (20-80μM) or UVB (30mJ/cm2) treatment, cells were cultured in 100μL 10% DMEM in each well for 24h. Cell viability was determined using the MTT assay. |
Reaction Conditions | 20-80μM; 24h |
Applications | trans-Zeatin (20-80μM) enhanced cell viability in HSFs irradiated with UVB. |
References: | |
| Cas No. | 1637-39-4 | SDF | |
| 别名 | 反-玉米素 | ||
| Canonical SMILES | OC/C(C)=C/CNC1=C2N=CNC2=NC=N1 | ||
| 分子式 | C10H13N5O | 分子量 | 219.24 |
| 溶解度 | DMSO : ≥ 60 mg/mL (273.67 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5612 mL | 22.8061 mL | 45.6121 mL |
| 5 mM | 912.2 μL | 4.5612 mL | 9.1224 mL |
| 10 mM | 456.1 μL | 2.2806 mL | 4.5612 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.50%
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