Thiazovivin
(Synonyms: Tzv) 目录号 : GC11181
Thiazovivin是一种选择性Rho相关激酶（ROCK）抑制剂。
Cas No.:1226056-71-8
Sample solution is provided at 25 µL, 10mM.
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Thiazovivin is a selective Rho-associated kinase (ROCK) inhibitor [1]. Thiazovivin selectively inhibits the ROCK signaling pathway, reduces cytoskeletal tension, reduces stress-induced cell apoptosis, promotes cell adhesion and spreading, and thus improves cell survival in a single-cell state [2-3]. Thiazovivin is often used in stem cell research and cell engineering [4].
In human corneal endothelial cells (HCEC), Thiazovivin (2µM, 4µM, 6µM; 24h, 48h) improve HCEC morphology, cell junctions, and ion transporter expression by inhibiting EndMT/EMT [5]. In human embryonic stem cells, Thiazovivin (2µM; 24h) treatment improves the maintenance of stemness in a putative stem-like cell populations of cattle by promoting the expression of pluripotency marker genes, as well as enhancing the expression of the E-cadherin gene, resulting in an increase in cell adhesion [1]. In human corneal endothelial cells, Thiazovivin (2µM, 4µM, 6µM; 24h) treatment improved cell morphology, connectivity and function, and significantly inhibited the expression of ROCK protein [6]. In bovine embryo, Thiazovivin (2µM; 96h) treatment improves embryo survival and hatching rates [7]. In human smooth muscle cells, Thiazovivin (2µM; 2h) reverses AngII-induced phosphorylation of MYPT1 and MLC [8].
References:
[1]. Park S, Kim D, Jung YG, et al. Thiazovivin, a Rho kinase inhibitor, improves stemness maintenance of embryo-derived stem-like cells under chemically defined culture conditions in cattle. Animal reproduction science. 2015 Oct 1; 161: 47-57.
[2]. Mohseni R, Shoae‐Hassani A, Verdi J. Reprogramming of endometrial adult stromal cells in the presence of a ROCK inhibitor, thiazovivin, could obtain more efficient iPSCs. Cell biology international. 2015 May; 39(5): 515-518.
[3]. Laowtammathron C, Chingsuwanrote P, Srisook P, et al. The novel combination of small-molecule inhibitors increases the survival and colony formation capacity of human induced pluripotent stem cells after single-cell dissociation. Science Progress. 2025 Apr; 108(2): 00368504251330956.
[4]. Rizzino A. Stimulating progress in regenerative medicine: improving the cloning and recovery of cryopreserved human pluripotent stem cells with ROCK inhibitors. Regenerative medicine. 2010 Sep 1; 5(5): 799-807.
[5]. Wu Q, Ouyang C, Xie L, et al. The ROCK inhibitor, thiazovivin, inhibits human corneal endothelial‑to‑mesenchymal transition/epithelial‑to‑mesenchymal transition and increases ionic transporter expression. International journal of molecular medicine. 2017 Oct 1; 40(4): 1009-1018.
[6]. Wu QN, Ling YZ, Ouyang C, et al. Effect of a new ROCK inhibitor thiazovivin on the morphology and function of human corneal endothelial cells. [Zhonghua yan ke za Zhi] Chinese Journal of Ophthalmology. 2016 Sep 1; 52(9): 686-692.
[7]. Kim D, Sul H, Jung YG, et al. Holding of bovine blastocysts at suprazero temperatures using small molecules. Scientific Reports. 2017 Aug 25; 7(1): 9490.
[8]. Cao X, Luo T, Luo X, et al. Resveratrol prevents AngII-induced hypertension via AMPK activation and RhoA/ROCK suppression in mice. Hypertension Research. 2014 Sep; 37(9): 803-810.
Thiazovivin是一种选择性Rho相关激酶（ROCK）抑制剂 [1]。Thiazovivin选择性抑制ROCK信号通路，降低细胞骨架张力，减少应激诱导的细胞凋亡，促进细胞粘附和扩散，从而提高单细胞状态下的细胞存活率 [2-3]。Thiazovivin常用于干细胞研究和细胞工程 [4]。
在人角膜内皮细胞（HCEC）中，Thiazovivin（2µM、4µM、6µM；24h、48h）通过抑制EndMT/EMT来改善HCEC的形态、细胞连接和离子转运蛋白的表达 [5]。在人类胚胎干细胞中，Thiazovivin（2µM；24h）处理可促进多能性标记基因的表达，并增强E-钙粘蛋白基因的表达，从而提高牛假定类干细胞群的干性维持，从而增加细胞粘附性 [1]。在人类角膜内皮细胞中，Thiazovivin（2µM、4µM、6µM；24h）处理可改善细胞形态、连通性和功能，并显著抑制ROCK蛋白的表达 [6]。在牛胚胎中，Thiazovivin（2µM；96h）处理可提高胚胎存活率和孵化率 [7]。在人类平滑肌细胞中，Thiazovivin（2µM；2h）可逆转血管紧张素Ⅱ诱导的MYPT1和MLC磷酸化 [8]。
| Cell experiment [1]: | |
Cell lines | Human corneal endothelial cells (HCECs) |
Preparation Method | Primary and passaged HCECs were cultured, and the cells were identified by observing the cell morphology under a light microscope and through NSE immunofluorescence staining experiments. When the confluence of primary cells was close to 70%-80%, 0.25% trypsin-EDTA (1X) was added for digestion. Following centrifugation, the cells were sub-cultured (1-3 generations). Primary and passaged HCECs were cultured onto 8-well chamber culture plates. When the cell confluence reached approximately 80%, various concentrations of Thiazovivin (2, 4 and 6µM), 0.6% DMSO, or 10µM Y-27632 were added. |
Reaction Conditions | 2µM, 4µM, 6µM; 24h, 48h |
Applications | Thiazovivin improve HCEC morphology, cell junctions, and ion transporter expression by inhibiting EndMT/EMT. |
References: | |
| Cas No. | 1226056-71-8 | SDF | |
| 别名 | Tzv | ||
| 化学名 | N-benzyl-2-(pyrimidin-4-ylamino)-1,3-thiazole-4-carboxamide | ||
| Canonical SMILES | C1=CC=C(C=C1)CNC(=O)C2=CSC(=N2)NC3=NC=NC=C3 | ||
| 分子式 | C15H13N5OS | 分子量 | 311.36 |
| 溶解度 | ≥ 15.55mg/mL in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2117 mL | 16.0586 mL | 32.1172 mL |
| 5 mM | 0.6423 mL | 3.2117 mL | 6.4234 mL |
| 10 mM | 0.3212 mL | 1.6059 mL | 3.2117 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet