Caffeic Acid Phenethyl Ester
(Synonyms: 咖啡酸苯乙酯) 目录号 : GC10692
Caffeic Acid Phenethyl Ester是一种天然存在蜂胶中的苯丙素类化合物，具有多种生物学活性。
Cas No.:104594-70-9
Sample solution is provided at 25 µL, 10mM.
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Caffeic Acid Phenethyl Ester is a naturally occurring phenylpropanoid compound found in propolis, possessing a variety of biological activities[1]. Caffeic Acid Phenethyl Ester potently and specifically inhibits the activation of the nuclear transcription factor NF-κB by preventing the nuclear translocation of its p65 subunit[2]. Caffeic Acid Phenethyl Ester exhibits significant antioxidant and anticancer activities[3-4].
In vitro, co-treatment of OV7, HTB76, and CRL1572 ovarian cancer cells with Caffeic Acid Phenethyl Ester (50–100μM) and 10nM paclitaxel (PTX) for 6–24 hours significantly enhanced the cytotoxicity of PTX and induced apoptosis[5]. Treatment of LPS (1μg/ml)-stimulated MDA-MB-231 cells with Caffeic Acid Phenethyl Ester (25–100μM) for 12–48 hours significantly inhibited cell proliferation, migration, and invasion, and reduced mitochondrial membrane potential[6].
In vivo, daily intraperitoneal injection of Caffeic Acid Phenethyl Ester (20–40mg/kg) for 24 days in a mouse model of experimental autoimmune encephalomyelitis (EAE) significantly reduced disease incidence and severity[7]. Daily intraperitoneal administration of Caffeic Acid Phenethyl Ester (20mg/kg) for 7 days to female C57BL/6 mice infected with enterotoxigenic Bacteroides fragilis(ETBF) significantly alleviated ETBF-induced colitis symptoms, including suppressing body weight loss, reducing spleen weight, and decreasing the colon weight-to-length ratio[8].
References:
[1] Murtaza G, Karim S, Akram MR, et al. Caffeic acid phenethyl ester and therapeutic potentials. Biomed Res Int. 2014;2014:145342.
[2] Pandey P, Khan F, Upadhyay TK, Therapeutic efficacy of caffeic acid phenethyl ester in cancer therapy: An updated review. Chem Biol Drug Des. 2023 Jul;102(1):201-216.
[3] Olgierd B, Kamila Ż, Anna B, et al. The Pluripotent Activities of Caffeic Acid Phenethyl Ester. Molecules. 2021 Mar 2;26(5):1335.
[4] Lv L, Cui H, Ma Z, et al. Recent progresses in the pharmacological activities of caffeic acid phenethyl ester. Naunyn Schmiedebergs Arch Pharmacol. 2021 Jul;394(7):1327-1339.
[5] Kleczka A, Dzik R, Kabała-Dzik A. Caffeic Acid Phenethyl Ester (CAPE) Synergistically Enhances Paclitaxel Activity in Ovarian Cancer Cells. Molecules. 2023 Aug 1;28(15):5813.
[6] Zhou Y, Wang J, Chang Y, et al. Caffeic Acid Phenethyl Ester Protects against Experimental Autoimmune Encephalomyelitis by Regulating T Cell Activities. Oxid Med Cell Longev. 2020 Oct 9;2020:7274342.
[7] Lan Z, Qu LJ, Liang Y, et al. AZD1390, an ataxia telangiectasia mutated inhibitor, attenuates microglia-mediated neuroinflammation and ischemic brain injury. CNS Neurosci Ther. 2024 Apr;30(4):e14696.
[8] Hwang S, Jo M, Hong JE, et al. Caffeic Acid Phenethyl Ester Administration Reduces Enterotoxigenic Bacteroides fragilis-Induced Colitis and Tumorigenesis. Toxins (Basel). 2024 Sep 18;16(9):403.
Caffeic Acid Phenethyl Ester是一种天然存在蜂胶中的苯丙素类化合物，具有多种生物学活性[1]。Caffeic Acid Phenethyl Ester能够强效且特异地抑制核转录因子NF-κB的活化，通过阻止NF-κB的p65亚单位进入细胞核来发挥作用[2]。Acid Phenethyl Ester有显著的抗氧化活性和抗癌活性[3-4]。
在体外，Caffeic Acid Phenethyl Ester（50–100μM）与10nM紫杉醇（PTX）联合处理OV7、HTB76和CRL1572卵巢癌细胞6–24小时，Caffeic Acid Phenethyl Ester显著增强紫杉醇的细胞毒性并诱导细胞凋亡[5]。Caffeic Acid Phenethyl Ester（25–100μM）处理经LPS（1μg/ml）刺激的MDA-MB-231细胞12–48小时，Caffeic Acid Phenethyl Ester显著抑制细胞增殖、迁移和侵袭，并降低线粒体膜电位[6]。
在体内，Caffeic Acid Phenethyl Ester（20–40mg/kg）每日腹腔注射实验性自身免疫性脑脊髓炎（EAE）小鼠模型24天，显著降低了疾病发生率和严重程度[7]。Caffeic Acid Phenethyl Ester（20mg/kg）每日腹腔注射处理经Bacteroides fragilis（ETBF）感染的C57BL/6雌鼠7天，显著减轻了ETBF诱导的结肠炎症状，包括抑制体重下降、减少脾脏重量及结肠重量/长度比值[8]。
| Cell experiment [1]: | |
Cell lines | MDA-MB-231 cells (human triple-negative breast cancer cell line) and HUVECs (human umbilical vein endothelial cells) |
Preparation Method | MDA-MB-231 cells were maintained in Leibovitz's L15 medium supplemented with 10% fetal bovine serum (FBS) at 37°C without CO₂. HUVECs were cultured in DMEM medium with 10% FBS at 37°C, 5% CO₂. Cells were treated with Caffeic Acid Phenethyl Ester at concentrations of 25, 50, and 100µM for 12, 24, and 48 hours in an inflammatory microenvironment stimulated with lipopolysaccharide (LPS; 1µg/mL). |
Reaction Conditions | 25–100µM; 12–48h |
Applications | Caffeic Acid Phenethyl Ester significantly inhibited cell viability, colony formation, migration, and invasion in MDA-MB-231 cells in a time- and dose-dependent manner. Caffeic Acid Phenethyl Ester suppressed angiogenesis in HUVECs by reducing tube formation, tube length, and junctions. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | Mice were orally inoculated with enterotoxigenic Bacteroides fragilis(ETBF; 1×10⁸CFU) after 5 days of antibiotic pretreatment (clindamycin and gentamicin). Caffeic Acid Phenethyl Ester (20mg/kg) was administered intraperitoneally daily for 7 days in the colitis model, or three times per week during two cycles of dextran sulfate sodium (DSS) treatment in the AOM/DSS-induced tumorigenesis model. |
Dosage form | 20mg/kg; i.p.; 7 days |
Applications | Caffeic Acid Phenethyl Ester administration significantly attenuated ETBF-induced colitis by reducing body weight loss, spleen weight, and colon weight-to-length ratio, while decreasing serum levels of pro-inflammatory cytokines (CXCL1 and IL-17A). |
References: | |
| Cas No. | 104594-70-9 | SDF | |
| 别名 | 咖啡酸苯乙酯 | ||
| 化学名 | 2-phenylethyl (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate | ||
| Canonical SMILES | C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O | ||
| 分子式 | C17H16O4 | 分子量 | 284.31 |
| 溶解度 | ≥ 28.4mg/mL in DMSO | 储存条件 | Store at -20° C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL |
| 5 mM | 703.5 μL | 3.5173 mL | 7.0346 mL |
| 10 mM | 351.7 μL | 1.7586 mL | 3.5173 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
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