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R6G phosphoramidite, 6-isomer Sale

(Synonyms: 罗丹明6G亚磷酰胺) 目录号 : GC59200

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer

R6G phosphoramidite, 6-isomer Chemical Structure

Cas No.:1355330-47-0

规格 价格 库存 购买数量
250mg
¥1,890.00
现货
1g
¥3,990.00
现货
5g
¥19,530.00
现货
10g
¥37,800.00
现货

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

R6G phosphoramidite for oligonucleotide synthesis, pure 6-isomer.

R6G (rhodamine 6G) is a xanthene dye of the rhodamine family with high fluorescence quantum yield and high molar extinction coefficient. Relative to those of fluorescein, the absorption (518 nm) and emission (542 nm) maxima of 6-R6G are shifted into the long-wave region.

R6G can be used as a FRET acceptor in DNA-sequencing and in short tandem repeat (STR) analysis. The dye is commonly used for producing TaqMan probes for quantitative PCR. In comparison with HEX and JOE dyes, R6G in combination with BHQ1 quencher has stronger quenching in molecular beacon probes, significantly reducing background fluorescence in PCR.

Usage:

Coupling: 10 minutes.

Deprotection: solution of tert-Butylamine in water (1:3 v/v) overnight at 55°С.

Aqueous ammonia and AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v) must NOT be used for deprotection of the modified oligonucleotide from the solid-phase support because of complete and irreversible R6G degradation.

Chemical Properties

Cas No. 1355330-47-0 SDF
别名 罗丹明6G亚磷酰胺
分子式 C46H54F6N5O8P 分子量 949.93
溶解度 good in DCM, acetonitrile 储存条件 Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 1.0527 mL 5.2635 mL 10.5271 mL
5 mM 0.2105 mL 1.0527 mL 2.1054 mL
10 mM 0.1053 mL 0.5264 mL 1.0527 mL
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