(2R)-Octyl-α-hydroxyglutarate ((2R)-Octyl-2-HG)
(Synonyms: (2R)-辛基-Α-羟基戊二酸酯,(2R)-Octyl-2-HG) 目录号 : GC32754A cell-permeable form of 2-HG
Cas No.:1391194-67-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
α-Hydroxyglutaric acid is normally metabolized to 2-oxoglutarate by D- and L-2-hydroxyglutarate dehydrogenases. Mutations in these enzymes cause 2-hydroxyglutaric aciduria, a neurometabolic disorder.1,2,3 Recent studies have found that mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2, typically associated with certain cancers, can cause these enzymes to convert isocitrate to 2-HG, rather than α-ketoglutarate.4,5 2-HG is structurally similar to α-ketoglutarate and competitively inhibits α-ketoglutarate-dependent dioxygenases, including lysine demethylases and DNA hydroxylases.5,6,7 (2R)-Octyl-α-hydroxyglutarate is a cell-permeable derivative of the D-isomer of 2-HG. It has been used to examine the contribution of D-2HG to the oxidative mitochondrial processes of IDH1-mutated cancer cells.8
1.Rzem, R., Veiga-da-Cunha, M., No?l, G., et al.A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduriaProc. Natl. Acad. Sci. USA101(48)16849-16854(2004) 2.Struys, E.A., Verhoeven, N.M., Roos, B., et al.Disease-related metabolites in culture medium of fibroblasts from patients with D-2-hydroxyglutaric aciduria, L-2-hydroxyglutaric aciduria, and combined D/L-2-hydroxyglutaric aciduriaClin. Chem.49(7)1133-1138(2003) 3.Struys, E.A., Salomons, G.S., Achouri, Y., et al.Mutations in the D-2-hydroxyglutarate dehydrogenase gene cause D-2-hydroxyglutaric aciduriaAm. J. Hum. Genet.76(2)358-360(2005) 4.Ward, P., Patel, J., Wise, D.R., et al.The common feature of leukemia-associated IDH1 and IDH2 mutations is a neomorphic enzyme activity converting α-ketoglutarate to 2-hydroxyglutarateCancer Cell17225-234(2010) 5.Yang, H., Ye, D., Guan, K.L., et al.IDH1 and IDH2 mutations in tumorigenesis: Mechanistic insights and clinical perspectivesClin. Cancer Res.18(20)5562-5571(2012) 6.Xu, W., Yang, H., Liu, Y., et al.Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenasesCancer Cell19(1)17-30(2011) 7.Chowdhury, R., Yeoh, K.K., Tian, Y.M., et al.The oncometabolite 2-hydroxyglutarate inhibits histone lysine demethylasesEMBO Rep.12(5)463-469(2011) 8.Reitman, Z.J., Duncan, C.G., Poteet, E., et al.Cancer-associated isocitrate dehydrogenase 1 (IDH1) R132H mutation and d-2-hydroxyglutarate stimulate glutamine metabolism under hypoxiaJ. Biol. Chem.289(34)23318-23328(2014)
Cas No. | 1391194-67-4 | SDF | |
别名 | (2R)-辛基-Α-羟基戊二酸酯,(2R)-Octyl-2-HG | ||
Canonical SMILES | O=C(OCCCCCCCC)[C@H](O)CCC(O)=O | ||
分子式 | C13H24O5 | 分子量 | 260.33 |
溶解度 | DMSO : 100 mg/mL (384.13 mM) | 储存条件 | Store at -20°C |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.8413 mL | 19.2064 mL | 38.4128 mL |
5 mM | 0.7683 mL | 3.8413 mL | 7.6826 mL |
10 mM | 0.3841 mL | 1.9206 mL | 3.8413 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。