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Cytokines(细胞因子)

Cytokines/immunocytokines, were initially used to separate the immunomodulatory proteins, also called immunotransmitters, from other growth factors that modulate proliferation and bioactivities of non-immune cells. Some cytokines are produced by a rather limited number of different cell types while others are produced by almost the entire spectrum of known cell types.

Recombinant cytokines are in clinical use, and continues attempts are made to develop hybrid molecules from cytokines. 1 must be aware of the fact that current knowledge is still limited. Cytokines are powerful two-edged weapons that can trigger a cascade of reactions, and may show activities that often go beyond the single highly specific property that it is hoped they possess. New factors are being discovered constantly and they extend our knowledge about the Cytokine network.

The understanding of the biological mechanisms governing cytokine actions are an important contribution to medical knowledge. The biochemistry and molecular biology of cytokine actions explain some well-known and sometimes also some of the more obscure clinical aspects of diseases. Knowledge that cytokines create regulatory hierarchies and provide independent and/or interrelated regulatory mechanisms that can confer distinct and interactive developmental functions lays a solid, albeit rather complicated foundation, for current and future clinical experiences.

The concept of "1 producer cell - 1 cytokine - 1 target cell" has been falsified for practically every cytokine investigated more closely. To classify these factors based on their producer or target cells is therefore also problematic.

Classifications based upon identical or shared biological activities of cytokines especially with broad definitions is also problematic for example: BCDF (B-cell differentiation factors), BCGF (B-cell growth factors), Motogenic cytokines, Chemotactic cytokines (Chemokines), CSF (colony stimulating factors), angiogenesis factors, or TRF (T-cell replacing factors). Designations such as HBGF group (heparin binding growth factors) take into account biochemical shared properties by a variety of cytokines which also problematic.

The term cytokine is used today as a generic name for a diverse group which includes proteins and peptides that act in nano-picomolar concentrations as humoral regulators and modulate the functional activities of individual cells & tissues. Cytokines also mediate interactions between cells & regulate processes taking place in the extracellular environment. Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death.

Cytokines resemble hormones in their biological activity & systemic level, for example, inflammation, systemic inflammatory response syndrome, acute phase reaction, wound healing, and the neuroimmune network.

Cytokines act on a wider spectrum of target cells than hormones. The major feature distinguishing cytokines from hormones is the fact that cytokines are not produced by specialized cells organized in specialized glands. Cytokines are secreted proteins which means that their expression sites does not predict where they exert their biological function.

Several cytokines primary structure was found to be identical with enzymes. Cytokines do not possess enzymatic activities although there is a growing list of exceptions. Other cytokines require proteolytic activation.

Cytokines include Interleukins, Lymphokines, Monokines, Interferons (IFN), colony stimulating factors (CSF), Chemokines and a variety of other proteins.

Type-1 cytokines are cytokines produced by Th1 T-helper cells while Type-2 cytokines are those produced by Th2 T-helper cells. Type-1 cytokines include IL-2 (IL2), IFN-gamma (IFN-G), IL-12 (IL12) & TNF-beta (TNF-b), while Type 2 cytokines include IL-4 (IL4), IL-5 (IL5), IL-6 (IL6), IL-10(IL10), and IL-13 (IL13).

Comparison of cytokine sequences shows that primate cytokines (non-human) are closely related. An example: IL-1 alpha (IL1a), IL-1 beta (IL1b), IL-2 (IL2), IL-4 (IL4), IL-5 (IL5), IL-6 (IL6) , IL8 (IL-8), IL-10 (IL10), IL-12 (IL12), IL-15 (IL15), IFN-alpha (IFNA), IFN-gamma (IFN-G), and TNF-alpha (TNFA) which share 93% to 99 % homology at the protein & nucleic acid level with the human sequences.

Cytokines can also be classified into family groups according to the types of secondary and tertiary structure. An example: IL-6 (IL6), IL-11 (IL11), CNTF (C-NTF), LIF, OSM (Oncostatin-M), EPO (Erythropoietin), G-CSF (GCSF), GH (Growth Hormone), PRL (Prolactin), IL-10 (IL10), IFN-alpha (IFN-A), IFN-beta (IFN-B) form long chain 4 helix bundles. IL-2 (IL2), IL-4 (IL4), IL-7 (IL7), IL-9 (IL9), IL-13 (IL13), IL-3 (IL3), IL-5 (IL5), GM-CSF (GMCSF), M-CSF (MCSF), SCF, IFN-gamma (IFNG) form short chain 4 helix bundles. Beta-trefoil structures are formed by IL1-alpha (IL1A), IL1-beta (IL1B), aFGF (FGF-acidic), bFGF (FGF-basic), INT-2 (INT2), KGF (FGF7). EGF, TGF-alpha (TGF-A), Betacellulin (BTC), SCDGF, Amphiregulin, HB-EGF, form EGF-like antiparallel beta-sheets.

Many cytokines are secreted by cells using secretory pathways and therefore are considered glycoproteins. Most genes encoding cytokines give rise to variety of cytokines by means of alternative splicing, yielding molecules with slightly different but biologically significant bioactivities. Usually the expression patterns of different forms of cytokines or of members of a cytokine family are overlapping only partially, suggesting a specific role for each factor.

Membrane-bound cytokines have been are associated with the extracellular matrix. The switching between soluble and membrane forms of cytokines is an important regulatory event. In some cases membrane forms of a cytokine have been found to be indispensable for normal development, with soluble forms being unable to entirely substitute for them.

Numerous cytokines are not stored inside cells though TGF-beta (TGF-b) and PDGF (P-DGF) are stored in platelets or TNF-alpha (TNF-A) and IL-8 (IL8) are found in human skin mast cells. Most of the cytokine’s expression is regulated tightly at practically all levels. The factors are usually produced only by cells after cell activation in response to an induction signal. The production and secretion of cytokines and growth factors frequently is context dependent, i.e., their expression is influenced by individual signals received but also by the balance of signals received through one or more receptors (which themselves may be subject to inducible/repressible expression).

Cytokine’s expression is regulated at the transcription level, translation level, and protein synthesis. The expression of cytokines also seems to be regulated differentially, depending on cell type and developmental age. Secretion or release from the producer cells is a regulated process. Once released, their behavior in the circulation may be regulated by soluble receptors and specific or unspecific binding proteins. Regulation also is at work at the receptor level on target cells and at the level of signaling pathways governing alterations in the behavior of responder cells.

Numerous cytokines are pleiotropic effectors showing multiple biological activities. Multiple cytokines have overlapping activities therefore a single cell frequently interacts with multiple cytokines with seemingly identical responses (cross-talk). A possible consequence of this functional overlap is the observation that 1 factor may frequently functionally replace another factor altogether or at least partially compensate for the lack of another factor. Since most cytokines have ubiquitous biological activities, their physiologic significance as normal regulators of physiology is often difficult to assess.

Gene function studies in experimental transgenic knock-out animals in which a cytokine gene has been functionally inactivated by gene targeting are very important in cytokine-research since, unlike in vitro studies, they provide information about the true in vivo functions of a given cytokine by highlighting the effects of their absence. In many instances these studies have shown that null mutations of particular cytokine genes do not have the effects in vivo expected from their activities in vitro.

Cytokines show stimulating or inhibitory activities and synergize or antagonize the actions of other factors. 1 sole Cytokine elicits reactions under certain circumstances that are the reverse of those shown under other circumstances. The type, the duration, and also the extent of cellular activities induced by a particular cytokine can be influenced considerably by the micro-environment of a cell, depending, for example, on the growth state of the cells (sparse or confluent), the type of neighboring cells, cytokine concentrations, the combination of other cytokines present at the same time, and even on the temporal sequence of several cytokines acting on the same cell. Under such circumstances combinatorial effects thus allow a single cytokine to transmit diverse signals to different subsets of cells.

Although some cytokines are known to share at least some biological effects, the observations that single cells usually show different patterns of gene expression in response to different cytokines can be taken as evidence for the existence of cytokine receptor-specific signal transduction pathways. Shared and different transcriptional activators that transduce a signal from a cytokine receptor to a transcription regulatory element of DNA are involved in these processes such as STAT proteins.

Basic FGF (bFGF) is a strong mitogen for fibroblasts at low concentrations and a chemoattractant at high concentrations. FGFb (FGF-b) has been shown also to be a biphasic regulator of human hepatoblastoma-derived HepG2 cells, depending upon the concentration. Interferon-gamma (IFN-gamma) can stimulate the proliferation of B-cells prestimulated with Anti-IgM, and inhibits the activities of the same cells induced by IL-4 (IL4). On the other hand, IL-4 (Interleukin-4) activates B-cells and promotes their proliferation while inhibiting the effects induced by IL2 in the same cells. The activity of at least two cytokines such as IL1-A (IL1A) & IL1-B (IL1B) is regulated by an endogenous receptor antagonist, the IL1 receptor anagonist (IL1TA). Cytokines, such as TNFA (TNF-A), IFN-gamma (IFN-G), IL-2 (IL2) & IL-4 (IL4), are inhibited by soluble receptors. Cytokines including IL-10 (IL10) and TGF-beta (TGF-B), inhibit other cytokines.

Early Cytokines preactivate cells so that they then can respond to late-acting cytokines. Cytokines induce the synthesis of novel gene products once they have bound to their corresponding. Several of the novel products are themselves cytokines. In addition, there are a variety of biological response modifiers that function as Anti-cytokines.

Cytokine mediators swiftly remote areas of a multicellular organism & multiple target cells can be degraded quickly, One can assume that cytokines play a pivotal role in all sorts of cell-to-cell communication processes although many of the mechanisms of their actions have not yet been elucidated in full detail.

Thorough examination of the physiological effects of the expression of cytokines in complex organisms has shown that these mediators are involved in all systemic reactions of an organism, including the important processes as regulation of immune responses, for example: BCDF(B-cell growth and differentiation factors), BCGF (B-cell growth factors) TRF (T-cell replacing factors), Isotype switching, inflammatory processes, hematopoiesis, and wound healing.

Embryogenesis and organ development inlvolves important mediators called Cytokines. Their activities in these processes may differ from those observed postnatally. Cytokines play a key role in neuroimmunological, neuroendocrinological, and neuroregulatory processes. Cytokines also regulate cell cycle, differentiation, migration, cell survival & cell death, and cell transformation. Viral infectious agents exploit the cytokine repertoire of organisms to evade immune responses of the host. Virus-encoded factors affect the activities of cytokines in at least four different ways: by inhibiting the synthesis and release of cytokines from infected cells; by interfering with the interaction between cytokines and their receptors; by inhibiting signal transmission pathways of cytokines; and by synthesizing virus-encoded cytokines that antagonize the effects of host cytokines mediating antiviral processes. Bacteria and micro-organisms also appear to produce substances with activities resembling those of cytokines and which they utilize to subvert host responses.

Cytokines are rarely related among eachother in their primary sequences. Some appear to have common 3 dimensional features and some of them can be grouped into families. An example is the TNF ligand superfamily members (with the exception of LT-alpha) are type 2 membrane glycoproteins (N-terminus inside) with homology to TNF in the extracellular domain (overall homologies, 20 %). The HBNF family includes members of the group of fibroblast growth factors. The chemokine group which contain diverse factors also have conserved sequence features. Analysis of crystal structures of several cytokines with very little sequence homology has revealed a common overall topology that is not deducible from sequence comparisons.

Cytokine biological activity of is mediated by specific membrane receptors, which are expressed on all cell types known. Cytokine expression is also subject to several regulatory although some receptors are expressed also constitutively.

Cytokine receptor proteins are multi-subunit structures that bind ligands and at the same time possess functions as signal transducers due to their intrinsic tyrosine kinase. Many receptors often share common signal transducing receptor components in the same family, which explains, at least in part, the functional redundancy of cytokines. Cross-communication between different signaling systems allows integration diversity of stimuli, which a cell can be subjected to under varying physiological situations. This and the ubiquitous cellular distribution of certain cytokine receptors have hampered attempts to define critical responsive cell populations and the physiologically important cell-specific functions of cytokines in vivo. Numerous receptors are associated with special signal transducing proteins in the interior of the cell. Receptors bind more than 1 cytokine. Cytokine receptors shown to be converted into soluble binding proteins that regulate ligand access to the cell by specific proteolytic cleavage of receptor ectodomains.

Specific activities of cytokines have been the basis for current concepts of therapeutical intervention, in particular of the treatment of hematopoietic malfunctions and tumor therapy. Applications involve the support of chemo- and radiotherapy, bone marrow transplantation, and general immunostimulation.

Products for  Cytokines

  1. Cat.No. 产品名称 Information
  2. GP20613 IL 20 Human Interleukin-20 Human Recombinant
  3. GP20612 IL 19 Mouse Interleukin-19 Mouse Recombinant
  4. GP20611 IL 19 Human Interleukin-19 Human Recombinant
  5. GP20610 IL18R1 Human Interleukin-18 Receptor-1 Recombinant Human
  6. GP20609 IL18RAP Human Interleukin 18 Receptor Accessory Protein Human Recombinant
  7. GP20608 IL 18 Mouse 白细胞介素 18 小鼠重组体
  8. GP20607 IL 18 Mouse, His Interleukin-18 Mouse Recombinant, His Tag
  9. GP20606 IL18BP Human, Sf9 Interleukin-18 Binding Protein Human Recombinant, Sf9
  10. GP20605 IL18BP Human Interleukin-18 Binding Protein Human Recombinant
  11. GP20604 IL 18 Human, His Interleukin-18 Human Recombinant, His Tag
  12. GP20603 il 18 Human 白介素 18 人重组体
  13. GP20602 IL17RB Mouse Interleukin-17 Receptor Beta Mouse Recombinant
  14. GP20601 IL17RB Human Interleukin-17 Receptor Beta Human Recombinant
  15. GP20600 IL17RA Human Interleukin-17 Receptor Alpha Human Recombinant
  16. GP20599 IL17F Rat Interleukin-17F Rat Recombinant
  17. GP20598 IL 17E Rat Interleukin-17E Rat Recombinant
  18. GP20597 IL 17 Rat Interleukin-17 Rat Recombinant
  19. GP20596 IL 17A/F Rat Interleukin-17A/F Heterodimer Rat Recombinant
  20. GP20595 IL 17 A/F Mouse Interleukin-17 A/F Heterodimer Mouse Recombinant
  21. GP20594 IL17F Mouse Interleukin-17F Mouse Recombinant
  22. GP20593 IL17E Mouse Interleukin-17E Mouse Recombinant
  23. GP20592 IL 17 Mouse Interleukin-17 Mouse Recombinant
  24. GP20591 IL 17A/F Human Interleukin-17A/F Heterodimer Human Recombinant
  25. GP20590 IL17F Human, sf9 Interleukin 17F Human Recombinant, sf9
  26. GP20589 IL 17F His Human Interleukin-17F Human Recombinant, His Tag
  27. GP20588 IL17F Human Interleukin 17F Human Recombinant
  28. GP20587 IL 17E Human Interleukin-17E Human Recombinant
  29. GP20586 IL17D Human

    白介素 17D 人类重组体

  30. GP20585 IL17B Human, Sf9 Interleukin-17B Human Recombinant, Sf9
  31. GP20584 IL17B Human, His Interleukin-17B Human Recombinant, His Tag
  32. GP20583 IL 17B Human Interleukin 17B Human Recombinant
  33. GP20582 IL17A Human, Sf9 Interleukin 17A Human Recombinant, Sf9
  34. GP20581 IL 17 Human, His Interleukin-17 Human Recombinant, His Tag
  35. GP20580 IL 17 Human Interleukin-17 Human Recombinant
  36. GP20579 IL 16 Rhesus Macaque Interleukin-16 Rhesus Macaque Recombinant
  37. GP20578 IL 16 Mouse Interleukin-16 Mouse Recombinant
  38. GP20577 IL 16 Human, His Interleukin-16 Human Recombinant, His Tag
  39. GP20576 IL 16 Human, (121 a.a.) Interleukin-16 Human Recombinant, (121 a.a.)
  40. GP20575 IL 16 Human, (130 a.a.) Interleukin-16 Human Recombinant, (130 a.a.)
  41. GP20574 IL15RA Human Interleukin 15 Receptor Alpha, Recombinant Human
  42. GP20573 IL 15 Rat Interleukin-15 Rat Recombinant
  43. GP20572 IL 15 Mouse, His Interleukin-15 Mouse Recombinant, His Tag
  44. GP20571 IL 15 Mouse 白细胞介素 15 小鼠重组体
  45. GP20570 IL15 Human, HEK Interleukin-15 Human Recombinant, HEK
  46. GP20569 IL 15 Human, His Interleukin-15 Human Recombinant, His Tag
  47. GP20568 IL 15 Human 白介素 15 人重组体
  48. GP20567 IL13RA2 Human, Sf9 Interleukin 13 Receptor Alpha 2, Recombinant Human Sf9
  49. GP20566 IL13RA2 Human Interleukin 13 Receptor, Alpha 2 Human Recombinant
  50. GP20565 IL13RA1 Human Interleukin 13 Receptor Alpha 1 Human Recombinant
  51. GP20564 IL 13 Rhesus Macaque Interleukin-13 Rhesus Macaque Recombinant

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