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PKA-RII alpha

目录号 : GP22593

cAMP-Dependent Protein Kinase A regulatory subunit-II A Recombinant

PKA-RII alpha Chemical Structure

规格 价格 库存 购买数量
1μg
¥840.00
5-10工作日
3μg
¥2,030.00
5-10工作日
100μg
¥51,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

The recombinant PKA regulatory subunit II-a is a dimeric 90 kDa protein. Protein Kinase A is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered clear solution. Shipping Condition Shipped with Ice Packs.
Synonyms cAMP-Dependent Protein Kinase A regulatory subunit-II A; PKA-RII alpha.
Amino Acid Sequence MSHIQIPPGL TELLQGYTVE VLRQQPPDLV EFAVEYFTRL REARAPASVL PAATPRQSLG HPPPEPGPDR VADAKGDSES EEDEDLEVPV PSRFNRRVSV CAETYNPDEE EEDTDPRVIH PKTDEQRCRL QEACKDILLF KNLDQEQLSQ VLDAMFERIV KADEHVIDQG DDGDNFYVIE RGTYDILVTK DNQTRSVGQY DNRGSFGELA LMYNTPRAAT IVATSEGSLW GLDRVTFRRI IVKNNAKKRK MFESFIESVP LLKSLEVSER MKIVDVIGEK IYKDGERIIT QGEKADSFYI IESGEVSILI RSRTKSNKDG GNQEVEIARC HKGQYFGELA LVTNKPRAAS AYAVGDVKCL VMDVQAFERL LGPCMDIMKR NISHYEEQLV KMFGSSVDLG NLGQ.
Stability PKA should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C . Avoid multiple freeze-thaw cycles.
Formulation PKA regulatory subunit-II alpha is supplied in 20mM MES, 150mM NaCl, 2mM EDTA, 2mM EGTA, 1mM BME and50% glycerol.

Introduction

cAMP-dependent PKA is a ubiquitous serine/theonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catatytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions.

Stability

PKA should be stored at 4°C if entire vial will be used within 2-4 weeks. For long term storage it is recommended to store at -20°C . Avoid multiple freeze-thaw cycles.