Oxy-16
(Synonyms: 20(R),22(R)-dihydroxy Cholesterol) 目录号 : GC17713A synthetic oxysterol
Cas No.:596-94-1
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Oxy-16 is an antagonist of hedgehog activity.
Naturally occurring oxysterols that are products of cholesterol oxidation can stimulate the hedgehog (Hh) signaling pathway related to cardiovascular disease and bone formation. Activation of Hh signaling modulates inflammatory responses to additional atherogenic factors including lesion-producing macrophages and enable osteoblast differentiation, which is dependent on the target cell.
In vitro: Oxysterols have been shown to be associated with immunosuppression, apoptosis, atherosclerosis, inflammation, and cholesterol turnover. Other studies aslo indicated that oxysterols were Hedgehog (Hh) signaling pathway activators and had potent osteoinductive properties. Oxy-16, an oxysterol, was identified as a cell permeable oxygenated derivative of cholesterol that might be the end product or intermediate of the cholesterol excretion pathways. Oxy-16 is currently used as a transport form for cholesterol across the blood brain barrier and membranes. The osteogenic differentiation caused by Oxy-16 was cinsidered to be mediated via a Wnt signaling-related, Dkk-1-inhibitable mechanism [1].
In vivo: Up to now, there is no animal in vivo data reported.
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] Amantea, C. M.,Kim, W.K.,Meliton, V., et al. Oxysterol-induced osteogenic differentiation of marrow stromal cells is regulated by Dkk-1 inhibitable and PI3-kinase mediated signaling. Journal of Cellular Biochemistry 105(2), 424-436 (2008).
| Cas No. | 596-94-1 | SDF | |
| 别名 | 20(R),22(R)-dihydroxy Cholesterol | ||
| 化学名 | cholest-5-ene-3b,20,22R-triol | ||
| Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])C2CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@@](C)(O)[C@H](O)CCC(C)C | ||
| 分子式 | C27H46O3 | 分子量 | 418.7 |
| 溶解度 | ≤10mg/ml in ethanol;1.5mg/ml in DMSO;1.5mg/ml in dimethyl formamide | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3883 mL | 11.9417 mL | 23.8834 mL |
| 5 mM | 0.4777 mL | 2.3883 mL | 4.7767 mL |
| 10 mM | 0.2388 mL | 1.1942 mL | 2.3883 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。