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NDK E.Coli

目录号 : GP22566

Nucleoside Diphosphate Kinase E.Coli Recombinant

NDK E.Coli Chemical Structure

规格 价格 库存 购买数量
5μg
¥840.00
5-10工作日
20μg
¥2,030.00
5-10工作日
1mg
¥37,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

NDK E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-143 a.a.) and having a molecular mass of 18kDa.NDK is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95% as determined by SDS-PAGE. Source E.coli.
Phycical Appearance Sterile Filtered colorless solution. Shipping Condition Shipped with Ice Packs.
Synonyms Nucleoside diphosphate kinase; NDK; NDP kinase; Nucleoside-2-P kinase; b2518; JW2502.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSHMAIERT FSIIKPNAVA KNVIGNIFAR FEAAGFKIVG TKMLHLTVEQ ARGFYAEHDG KPFFDGLVEF MTSGPIVVSV LEGENAVQRH RDLLGATNPA NALAGTLRAD YADSLTENGT HGSDSVESAA REIAYFFGEG EVCPRTR.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Formulation NDK protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT.

Introduction

Nucleoside diphosphate kinase (Ndk) is an enzyme which catalyzes the exchange of phosphate groups between different nucleoside diphosphates. Ndkбпs activity sustains equilibrium between the concentrations of various nucleoside triphosphates for example, when GTP is produced in the citric acid (Krebs) cycle is converted to ATP. Ndk has a key role in the synthesis of nucleoside triphosphates other than ATP.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.