MGR2
目录号 : GC44190
A negative control for MGR1
Cas No.:2361529-47-5
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
MGR2 is a negative control compound for the activity of the reactive oxygen species-generating probe MGR1. Unlike MGR1, MGR2 does not induce superoxide production or cell death in HEK293T cells.
| Cas No. | 2361529-47-5 | SDF | |
| Canonical SMILES | O=C(C(C)(C)C)OCOC1=C(C=CC=C2)C2=CC=C1 | ||
| 分子式 | C16H18O3 | 分子量 | 258.3 |
| 溶解度 | DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 3.8715 mL | 19.3573 mL | 38.7147 mL |
| 5 mM | 0.7743 mL | 3.8715 mL | 7.7429 mL |
| 10 mM | 0.3871 mL | 1.9357 mL | 3.8715 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
MGR2 regulates mitochondrial preprotein import by associating with channel-forming Tim23 subunit
Mol Biol Cell 2020 May 15;31(11):1112-1123.PMID:32186971DOI:10.1091/mbc.E19-12-0677.
MGR2, a newly identified subunit of the TIM23 complex, functions as a gatekeeper of presequence translocase and thereby maintains quality control of inner membrane preproteins sorting. However, precise recruitment of the MGR2 subunit to the core channel and how it influences the assembly of the TIM23 complex during lateral sorting of preproteins are poorly understood. Present findings provide insights into a direct association of MGR2 with the channel-forming Tim23 subunit. Furthermore, the mutational analysis uncovers the TM1 region of MGR2 critically required for association with Tim23 and Tim21. On the other hand, the TM2 region of MGR2 is involved in bridging respiratory complexes to the TIM23 complex via Tim21. Importantly, both TM regions of MGR2 are essential for lateral sorting of preprotein into the inner membrane, as well as maintaining mitochondrial morphology. Together, our findings provide mechanistic insights into the role of MGR2 in regulating the dynamicity of the TIM23 complex assembly required for preprotein import and coupling of respiratory pathways.
The MGR2 subunit of the TIM23 complex regulates membrane insertion of marginal stop-transfer signals in the mitochondrial inner membrane
FEBS Lett 2020 Mar;594(6):1081-1087.PMID:31764998DOI:10.1002/1873-3468.13692.
The TIM23 complex mediates membrane insertion of presequence-containing mitochondrial proteins via a stop-transfer mechanism. Stop-transfer signals consist of hydrophobic transmembrane segments and flanking charges. MGR2 functions as a lateral gatekeeper of the TIM23 complex. However, it remains elusive which features of stop-transfer signals are discriminated by MGR2. To determine the effects of MGR2 on the TIM23-mediated stop-transfer pathway, we measured membrane insertion of model transmembrane segments of varied hydrophobicity and flanking charges in Mgr2-deletion or -overexpression yeast strains. We found that upon deletion of MGR2, the threshold hydrophobicity for membrane insertion, as well as the requirement for matrix-facing positive charges, is reduced. These results imply that the Mgr2-mediated gatekeeper function is important for controlling membrane sorting of marginal stop-transfer signals.
MGR2 functions as lateral gatekeeper for preprotein sorting in the mitochondrial inner membrane
Mol Cell 2014 Dec 4;56(5):641-52.PMID:25454944DOI:10.1016/j.molcel.2014.10.010.
The majority of preproteins destined for mitochondria carry N-terminal presequences. The presequence translocase of the inner mitochondrial membrane (TIM23 complex) plays a central role in protein sorting. Preproteins are either translocated through the TIM23 complex into the matrix or are laterally released into the inner membrane. We report that the small hydrophobic protein MGR2 controls the lateral release of preproteins. MGR2 interacts with preproteins in transit through the TIM23 complex. Overexpression of MGR2 delays preprotein release, whereas a lack of MGR2 promotes preprotein sorting into the inner membrane. Preproteins with a defective inner membrane sorting signal are translocated into the matrix in wild-type mitochondria but are released into the inner membrane in Mgr2-deficient mitochondria. We conclude that MGR2 functions as a lateral gatekeeper of the mitochondrial presequence translocase, providing quality control for the membrane sorting of preproteins.
MGR2 promotes coupling of the mitochondrial presequence translocase to partner complexes
J Cell Biol 2012 May 28;197(5):595-604.PMID:22613836DOI:10.1083/jcb.201110047.
Many mitochondrial proteins are synthesized with N-terminal presequences in the cytosol. The presequence translocase of the inner mitochondrial membrane (TIM23) translocates preproteins into and across the membrane and associates with the matrix-localized import motor. The TIM23 complex consists of three core components and Tim21, which interacts with the translocase of the outer membrane (TOM) and the respiratory chain. We have identified a new subunit of the TIM23 complex, the inner membrane protein MGR2. Mitochondria lacking MGR2 were deficient in the Tim21-containing sorting form of the TIM23 complex. MGR2 was required for binding of Tim21 to TIM23(CORE), revealing a binding chain of TIM23(CORE)-Mgr2/Tim21-respiratory chain. Mgr2-deficient yeast cells were defective in growth at elevated temperature, and the mitochondria were impaired in TOM-TIM23 coupling and the import of presequence-carrying preproteins. We conclude that MGR2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import.
The great escape: MGR2 of the mitochondrial TIM23 translocon is a gatekeeper Tasked with releasing membrane proteins
Mol Cell 2014 Dec 4;56(5):613-4.PMID:25479635DOI:10.1016/j.molcel.2014.11.022.
By using a combination of biochemistry and genetics, in this issue of Molecular CellIeva et al. (2014) uncover an unexpected role for MGR2 of the mitochondrial TIM23 translocon as a gatekeeper in the release of membrane proteins from the translocon.