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MGLL Human, Active

目录号 : GP21922

Monoglyceride Lipase Human Recombinant, Active

MGLL Human, Active Chemical Structure

规格 价格 库存 购买数量
1μg
¥840.00
5-10工作日
5μg
¥2,030.00
5-10工作日
50μg
¥16,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

MGLL Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 333 amino acids (1-313 a.a.) and having a molecular mass of 36.4kDa. The MGLL is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 85.0% as determined by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered colorless solution. Shipping Condition Shipped with Ice Packs.
Synonyms Monoglyceride lipase; MGL; HU-K5; Lysophospholipase homolog; Lysophospholipase-like; Monoacylglycerol lipase; MAGL; MGLL; HUK5.
Amino Acid Sequence MGSSHHHHHH SSGLVPRGSHMETGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDLLVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFAGMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFGIQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTNSVFHEINMWV SQRTATAGTA SPP.
Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Biological Activity Specificactivity is > 170 units/mg, and is defined as the amount of enzyme that hydrolyze1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at25C.
Formulation The MGLL solution (0.5mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0) and 10% Glycerol.

Introduction

MGLL is a membrane-associated member of the serine hydrolase superfamily. MGLL is expressed in abundance in skeletal muscle and adipose tissue. MGLL functions jointly with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides.

Biological Activity

Specificactivity is > 170 units/mg, and is defined as the amount of enzyme that hydrolyze1.0 umole of p-nitrophenyl butyrate to pnitrophenol per minute at pH 7.5 at25C.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.