M CSF Human
目录号 : GP20732Macrophage-Colony Stimulating Factor Human Recombinant
Sample solution is provided at 25 µL, 10mM.
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Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KD.MCSF is purified by proprietary chromatographic techniques.
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Macrophage Colony Stimulating Factor; CSF-1; Lanimostim; MCSF; MGC31930; M-CSF. | ||
| Amino Acid Sequence | MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS. | ||
| Solubility | It is recommended to reconstitute the lyophilized MCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles. | ||
| Biological Activity | The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found to be < 1.4-2.1ng/ml. | ||
| Formulation | The MCSF protein was lyophilized with 10mM sodium Phosphate, pH-8.0 & 50mM NaCl. | ||
Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. MCSF induces cells of the monocyte/macrophage lineage. MCSF plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
The ED50, calculated by the dose-dependent stimulation of the proliferation of murine M-NFS-60 indicator cells was found to be < 1.4-2.1ng/ml.
Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.