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Leptin Salamander

目录号 : GP20699

Leptin Salamander Recombinant

Leptin Salamander Chemical Structure

规格 价格 库存 购买数量
100μg
¥4,900.00
5-10工作日
200μg
¥7,000.00
5-10工作日
1mg
¥23,800.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Leptin Salamander Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of 16kDa. The Salamander Leptin is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 99.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms OB Protein; Obesity Protein; OBS; Obesity factor; Leptin.
Amino Acid Sequence The sequence of the ten N-terminal amino acids was determined and was found to be Ala-Ile-Met-Val-Asp-Gln-Leu-Arg-Met-Asp.
Solubility It is recommended to reconstitute the lyophilized Salamander Leptin in sterile 0.4% NaHCO3 pH-9 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Salamander Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution Leptin should be stored at 4?C between 2-7 days and for future use below -18?C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity By inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128иC136).
Formulation The Salamander Leptin was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Introduction

A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

Biological Activity

By inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity in that test is similar to that of mouse leptin (see Salomon et al (2006) Protein Expression and Purifcation 47, 128иC136).

Stability

Lyophilized Salamander Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18?C. Upon reconstitution Leptin should be stored at 4?C between 2-7 days and for future use below -18?C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.