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Leptin Human, N82K

目录号 : GP20688

Leptin Human Recombinant, N82K

Leptin Human, N82K Chemical Structure

规格 价格 库存 购买数量
50μg
¥840.00
5-10工作日
200μg
¥2,030.00
5-10工作日
1mg
¥4,200.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

Leptin N82K Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids (and additional Ala at N-terminus acids) and having a molecular mass of 16kDa.The Leptin N82K is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 98.0% as determined by:(a)Gel filtration analysis.(b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms OB Protein; Obesity Protein; OBS; Obesity factor.
Amino Acid Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln.
Solubility It is recommended to reconstitute the lyophilized leptin N82K in sterile water or 0.4% NaHCO3 adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized leptin N82K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution leptin N82K should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Biological Activity Biological Activity is < than 0.1% as determined by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
Formulation Lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Introduction

Leptin takes an important part in the regulation of energy balance and body weight control.After entering the circulation, Leptin binds LEPRwhich results in the activation of several major signalling pathways. In the hypothalamus Leptin acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption and also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, regulates pancreatic beta-cell function and insulin secretion and affects innate and adaptive immunity.

Biological Activity

Biological Activity is < than 0.1% as determined by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

Stability

Lyophilized leptin N82K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution leptin N82K should be stored at 4°C between 2-7 days and for future use below -18°C . For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.