LDHB
目录号 : GP21875Lactate Dehydrogenase B Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile lyophilized powder. | Shipping Condition | Shipped at Room temp. |
| Synonyms | Lactate Dehydrogenase; LDH. | ||
| Solubility | It is recommended to reconstitute the lyophilized LDH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. | ||
| Stability | Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles. | ||
| Biological Activity | The specific activity was found to be 258 U/mg protein. | ||
| Formulation | The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate. | ||
Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
The specific activity was found to be 258 U/mg protein.
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution LDH should be stored at 4°C between 2-7 days and for future use below -18°C .Please prevent freeze-thaw cycles.