LDHA Mouse
目录号 : GP21873Lactate Dehydrogenase A Mouse Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 95.0% as determined by SDS-PAGE. | Source | Sf9,Baculovirus cells. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | L-lactatedehydrogenase A chain; LDH-A; LDH muscle subunit; LDH-M. | ||
| Amino Acid Sequence | MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELALVDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFIIPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGVHALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEVHKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGISDVVKVTLTP EEEARLKKSA DTLWGIQKEL QFLEHHHHHH. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Biological Activity | Specificactivity is > 250 units/mg, and is defined as the Amount of enzyme thatconvert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C. | ||
| Formulation | LDHA protein solution (0.5mg/ml) containing PhosphateBuffered Saline (pH 7.4) and 10% glycerol. | ||
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.
Specificactivity is > 250 units/mg, and is defined as the Amount of enzyme thatconvert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37C.
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.