Iso-Isariin B
目录号 : GC47465
A fungal metabolite
Cas No.:1290627-99-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >70.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Iso-isariin B is a cyclodepsipeptide fungal metabolite originally isolated from B. felina that has insecticidal activity.1 It induces mortality in Sitophilus adults with an LD50 value of 10 µg/ml.
1.Langenfeld, A., Blond, A., Gueye, S., et al.Insecticidal cyclodepsipeptides from Beauveria felinaJ. Nat. Prod.74(4)825-830(2011)
| Cas No. | 1290627-99-4 | SDF | |
| Canonical SMILES | O=C(N[C@@H](C(C)C)C(OC(C(C)CCCC)CC1=O)=O)[C@H](C)NC([C@H](NC([C@@H](NC(CN1)=O)C(C)C)=O)CC(C)C)=O | ||
| 分子式 | C30H53N5O7 | 分子量 | 595.8 |
| 溶解度 | Dichloromethane: soluble,DMSO: soluble,Ethanol: soluble,Methanol: soluble | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6784 mL | 8.3921 mL | 16.7842 mL |
| 5 mM | 0.3357 mL | 1.6784 mL | 3.3568 mL |
| 10 mM | 0.1678 mL | 0.8392 mL | 1.6784 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungus Amphichorda guana
Org Biomol Chem 2021 Mar 11;19(9):1960-1964.PMID:33599675DOI:10.1039/d1ob00100k.
Two potential non-ribosomal peptide synthetases (NRPSs) were identified in the genome of a guanophilic fungus Amphichorda guana by bioinformatics analysis and gene knockout experiments. Liquid chromatography coupled with mass spectrometry (LC-MS) guided isolation led to the discovery of a new cyclodepsipeptide isaridin H (1) and seven known analogs, desmethylisaridin E (2), isaridin E (3), isariin A (4), Iso-Isariin B (5), iso-isariin D (6), isariin E (7), and nodupetide (8). The absolute configuration of isaridin H (1) was achieved by Marfey's method. Isaridin H (1) showed significant antifungal activity against Botrytis cinerea and Alternaria solani.
Insecticidal cyclodepsipeptides from Beauveria felina
J Nat Prod 2011 Apr 25;74(4):825-30.PMID:21438588DOI:10.1021/np100890n.
A novel cyclodepsipeptide, Iso-Isariin B (1), and the known isaridin E (2) were isolated from the entomopathogenic fungus Beauveria felina. Their structures were elucidated using MS/MS fragmentation and extensive 2D-heteronuclear NMR. The X-ray structure of isaridin E was obtained, showing two potent intramolecular H bonds and a type-VI turn with the HyLeu(1)-Pro(2) amide bond in a cis conformation. Iso-Isariin B (1) was active against the pest-insect Sitophilus spp. with an LD(50) value of 10 μg/mL. This observation also gives some clues for ecological interpretation of cyclodepsipeptide production by B. felina.