Inulicin (1-O-Acetylbritannilactone)
(Synonyms: 旋覆花内酯,1-O-Acetylbritannilactone) 目录号 : GC33861
A sesquiterpene lactone with diverse biological activities
Cas No.:33627-41-7
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment: | HUVECs or A549 cells are plated in 60 mm plates (300 cells/plate). After overnight incubation, cells are treated with applied agents (e.g., Inulicin; 5 μM and 10 μM) for 24 h. Cells are then washed, and fresh media are added. After 10 days of incubation, surviving colonies are fixed, stained, and manually counted[1]. |
Animal experiment: | Mice[1]Male nude mice (4-6weeks old, BALB/c) are used. A549 cells (five million cells in 0.1 mL of culture medium) are subcutaneously injected at the right thigh of nude mice, and treatment is started when the tumors reach an average volume about 100 mm3. Animals are randomized into two groups with 10 mice per group: (a) Vehicle; (b) 12 mg/kg of Inulicin. Inulicin is injected intraperitoneally (i.p.) daily. The mice are examined daily for toxicity/mortality relevant to treatment, and the tumor is measured with a caliper every two days. The tumor volume (in mm3) is calculated, and the tumor growth curve is presented. At the end of experiments, xenograft tumors are isolated through surgery and weighted[1]. |
References: [1]. Zhengfu H, et al. 1-o-acetylbritannilactone (ABL) inhibits angiogenesis and lung cancer cell growth through regulating VEGF-Src-FAK signaling. Biochem Biophys Res Commun. 2015 Aug 21;464(2):422-7. | |
1-O-Acetylbritannilactone is a sesquiterpene lactone that has been found in I. britannica and has diverse biological activities.1,2 It inhibits LPS-induced production of prostaglandin E2 and nuclear translocation of NF-κB in primary rat aorta vascular smooth muscle cells (VSMCs).1 1-O-Acetylbritannilactone (5 and 10 ?M) inhibits VEGF-induced capillary tube formation in human umbilical vein endothelial cells (HUVECs).2 It reduces tumor growth in an A549 mouse xenograft model when administered at a dose of 12 mg/kg per day.
1.Liu, Y.-P., Wen, J.-K., Zheng, B., et al.Acetylbritannilactone suppresses lipopolysaccharide-induced vascular smooth muscle cell inflammatory responseEur. J. Pharmacol.577(1-3)28-34(2007) 2.Zhengfu, H., Hu, Z., Huiwen, M., et al.1-o-acetylbritannilactone (ABL) inhibits angiogenesis and lung cancer cell growth through regulating VEGF-Src-FAK signalingBiochem. Biophys. Res. Commun.464(2)422-427(2015)
| Cas No. | 33627-41-7 | SDF | |
| 别名 | 旋覆花内酯,1-O-Acetylbritannilactone | ||
| Canonical SMILES | O[C@H]1[C@@](C2=C)([H])[C@@](OC2=O)([H])CC(C)=C1[C@@H](C)CCCOC(C)=O | ||
| 分子式 | C17H24O5 | 分子量 | 308.37 |
| 溶解度 | Ethanol : 50 mg/mL (162.14 mM) | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2429 mL | 16.2143 mL | 32.4286 mL |
| 5 mM | 0.6486 mL | 3.2429 mL | 6.4857 mL |
| 10 mM | 0.3243 mL | 1.6214 mL | 3.2429 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。