Gramicidin A
(Synonyms: 甲胎蛋白) 目录号 : GC40918A channel-forming ionophore
Cas No.:11029-61-1
Sample solution is provided at 25 µL, 10mM.
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Gramicidin A is a peptide component of gramicidin, an antibiotic mixture originally isolated from B. brevis. Gramicidin A is a highly hydrophobic channel-forming ionophore that forms channels in model membranes that are permeable to monovalent cations. It induces degradation of hypoxia inducible factor 1 α (HIF-1α) in HEK293 cells and reduces growth in a human renal cell carcinoma mouse xenograft model when administered at a dose of 0.22 mg/kg three times per week. Gramicidin A has commonly been used to study channel structure and function.
Cas No. | 11029-61-1 | SDF | |
别名 | 甲胎蛋白 | ||
分子式 | C99H140N20O17 | 分子量 | 1882.3 |
溶解度 | DMSO: soluble,Ethanol: soluble,Water: slightly soluble | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 0.5313 mL | 2.6563 mL | 5.3126 mL |
5 mM | 0.1063 mL | 0.5313 mL | 1.0625 mL |
10 mM | 0.0531 mL | 0.2656 mL | 0.5313 mL |
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2.
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Gramicidin A is hydrolyzed by a d-stereospecific peptidase produced by Bacillus anthracis
Environ Microbiol Rep 2022 Aug;14(4):570-576.PMID:35403341DOI:10.1111/1758-2229.13069.
Previously we described the discovery of a Bacillus spp. specific peptidase activity related to d-stereospecific peptidases (DSPs). The peptidase showed a strong preference for d-leucine and d-valine amino acids. These amino acids are present in the structure of the non-ribosomal peptide (NRP) antibiotics Gramicidin A, B and C and polymyxin E. To examine if the Bacillus spp. DSP-related peptidase can hydrolyze these NRPs, the effect of Gramicidin A and C and polymyxin E on peptidase activity in Bacillus anthracis culture supernatant was monitored. It was found that both gramicidins inhibited the DSP-related activity in a competitive manner. MALDI-TOF analysis revealed that upon incubation with B. anthracis culture supernatant Gramicidin A hydrolyzation products appeared. This study shows that the Bacillus spp. specific DSP-like peptidase was potentially produced by the bacteria to gain intrinsic resistance against NRP antibiotics. These results are of utmost importance in research towards antimicrobial resistance, whereas transfer of DSP-related activity to other clinically relevant pathogens can be a serious threat to human health.
Gramicidin A--phospholipid model systems
J Bioenerg Biomembr 1987 Dec;19(6):655-76.PMID:2447070DOI:10.1007/BF00762301.
Gramicidin A forms ion-conducting channels which can traverse the hydrocarbon core of lipid bilayer membranes. The structures formed by Gramicidin A are among the best characterized of all membrane-bound polypeptides or proteins. In this review a brief summary is given of the occurrence, conformation, and synthesis of Gramicidin A, and of its use as a model for ion transport and the interaction of proteins and lipids in biological membranes.
Effects of Calcium Ions on the Antimicrobial Activity of Gramicidin A
Biomolecules 2022 Dec 1;12(12):1799.PMID:36551225DOI:10.3390/biom12121799.
Gramicidin A (gA) is a linear antimicrobial peptide that can form a channel and specifically conduct monovalent cations such as H+ across the lipid membrane. The antimicrobial activity of gA is associated with the formation of hydroxyl free radicals and the imbalance of NADH metabolism, possibly a consequence caused by the conductance of cations. The ion conductivity of Gramicidin A can be blocked by Ca2+ ions. However, the effect of Ca2+ ions on the antimicrobial activity of gA is unclear. To unveil the role of Ca2+ ions, we examined the effect of Ca2+ ions on the antimicrobial activity of Gramicidin A against Staphylococcus aureus (S. aureus). Results showed that the antimicrobial mechanism of gA and antimicrobial activity by Ca2+ ions are concentration-dependent. At the low gA concentration (≤1 μM), the antimicrobial mechanism of gA is mainly associated with the hydroxyl free radical formation and NADH metabolic imbalance. Under this mode, Ca2+ ions can significantly inhibit the hydroxyl free radical formation and NADH metabolic imbalance. On the other hand, at high gA concentration (≥5 μM), Gramicidin A acts more likely as a detergent. Gramicidin A not only causes an increase in hydroxyl free radical levels and NAD+/NADH ratios but also induces the destruction of the lipid membrane composition. At this condition, Ca2+ ions can no longer reduce the gA antimicrobial activity but rather enhance the bacterial killing ability of Gramicidin A.
Gramicidin A-based unimolecular channel: cancer cell-targeting behavior and ion transport-induced apoptosis
Chem Commun (Camb) 2021 Feb 2;57(9):1097-1100.PMID:33443269DOI:10.1039/d0cc08073j.
A series of glycoside-peptide conjugates were prepared by engineering at the N-terminus of the natural peptide Gramicidin A. The conjugate containing galactose moiety formed a unimolecular transmembrane channel and mediated ion transport to induce apoptosis of cancer cells. More importantly, it exhibited liver cancer cell-targeting behavior due to the galactose-asialoglycoprotein receptor recognition.
The binding site of sodium in the Gramicidin A channel
Novartis Found Symp 1999;225:113-24; discussion 124-7.PMID:10472051DOI:10.1002/9780470515716.ch8.
The available information concerning the structure and location of the main binding site for sodium in the Gramicidin A channel is reviewed and discussed. Results from molecular dynamics simulations using an atomic model of the channel embedded in a lipid bilayer are compared with experimental observations. The combined information from experiment and simulation suggests that the main binding sites for sodium are near the channel's mouth, approximately 9.2 A from the centre of the dimer channel, although the motion along the axis could be as large as 1 to 2 A. In the binding site, the sodium ion is lying off axis, making contact with two carbonyl oxygens and two single-file water molecules. The main channel ligand is provided by the carbonyl group of the Leu10-Trp11 peptide linkage, which exhibits the largest deflection from the ion-free channel structure.