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GPT Human, Active

目录号 : GP21719

Glutamic-Pyruvate Transaminase Human Recombinant, Active

GPT Human, Active Chemical Structure

规格 价格 库存 购买数量
1U
¥840.00
5-10工作日
3U
¥2,030.00
5-10工作日
50U
¥25,200.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

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产品描述

Alanine Aminotransferase Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT.The ALT is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile liquid formulation. Shipping Condition Shipped with Ice Packs.
Synonyms Alanine aminotransferase 1; ALT1; EC 2.6.1.2; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT; AAT1; GPT1.
Stability AAT1 although stable at 10°C for 5 days, should be stored below -18°C .Please prevent freeze-thaw cycles.
Biological Activity The specific activity was found to be 1,020 U/mg.
Formulation The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.

Introduction

Alanine transaminase or ALT is a transaminaseenzyme.ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamatealanine+ a-ketoglutarate= pyruvate+ glutamateIt is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L).

Biological Activity

The specific activity was found to be 1,020 U/mg.

Stability

AAT1 although stable at 10°C for 5 days, should be stored below -18°C .Please prevent freeze-thaw cycles.