GlpK E. coli
目录号 : GP22525Glycerol kinase E. Coli Recombinant
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
| Purity | Greaterthan 95.0% as determined by SDS-PAGE. | Source | Escherichia Coli. |
| Phycical Appearance | Sterile Filtered clear solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Glycerolkinase; glycerol 3-phosphotransferase; Glycerokinase; GK. | ||
| Amino Acid Sequence | MGSSHHHHHHSSGLVPRGSH MGSMTEKKYIVALDQGTTSS RAVVMDHDAN IISVSQREFE QIYPKPGWVE HDPMEIWATQ SSTLVEVLAK ADISSDQIAAIGITNQRETT IVWEKETGKP IYNAIVWQCR RTAEICEHLK RDGLEDYIRS NTGLVIDPYF SGTKVKWILDHVEGSRERAR RGELLFGTVD TWLIWKMTQG RVHVTDYTNA SRTMLFNIHT LDWDDKMLEV LDIPREMLPEVRRSSEVYGQ TNIGGKGGTR IPISGIAGDQ QAALFGQLCV KEGMAKNTYG TGCFMLMNTG EKAVKSENGL LTTIACGPTGEVNYALEGAV FMAGASIQWL RDEMKLINDA YDSEYFATKV QNTNGVYVVP AFTGLGAPYW DPYARGAIFGLTRGVNANHI IRATLESIAY QTRDVLEAMQ ADSGIRLHAL RVDGGAVANN FLMQFQSDIL GTRVERPEVREVTALGAAYL AGLAVGFWQN LDELQEKAVI EREFRPGIET TERNYRYAGW KKAVKRAMAW EEHDE. | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Formulation | GlpK protein solution (1mg/ml) containing Phosphatebuffered saline (pH7.4),10% glycerol and 1mM DTT. | ||
GlpKalso known as glycerol kinase, is a member of the FGGY kinase family. GlpKcatalyzes the transfer of a phosphate group from ATP to glycerol, therebyforming glycerol phosphate. Furthermore, this intermediate can then beconverted to dihydroxyacetone phosphate (DHAP), which is utilized in eitherglycolysis or gluconeogenesis. The activity of GlpK is affected by numerous metabolites.The non-competitive allosteric inhibition by fructose 1,6-bisphosphate (FBP) triggersmodifications in the quaternary structure of Glpk.
Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.