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GCSF Rat

目录号 : GP20324

Granulocyte-Colony Stimulating Factor Rat Recombinant

GCSF Rat Chemical Structure

规格 价格 库存 购买数量
2μg
¥840.00
5-10工作日
10μg
¥2,030.00
5-10工作日
1mg
¥65,520.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

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产品描述

GCSF Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids and having a molecular mass of 21.5kDa.The G-CSF is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 97.0% as determined by:(a)Analysis by RP-HPLC.(b)Analysis by SDS-PAGE. Source Escherichia Coli.
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Granulocyte colony stimulating factor; Protein Csf3; Csf3.
Amino Acid Sequence KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI.
Solubility It is recommended to reconstitute the lyophilized GCSF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of > 2.0б┴ 107 IU/mg.
Formulation Lyophilized from a 0.2µm filtered solution in 5mM Sodium Citrate, pH 4.0.

Introduction

GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes.

Biological Activity

The ED50 determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml, corresponding to a specific activity of > 2.0б┴ 107 IU/mg.

Stability

Lyophilized G-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.