NBD-Fructose
(Synonyms: 1-NBDF, 1-(7-nitro-1,2,3-benzadiazole)-Fructose) 目录号 : GC45521A fluorescent derivative of fructose
Cas No.:940961-04-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
NBD-Fructose is a fluorescent derivative of fructose that is formed by coupling NBD-chloride with the amine group of amino fructose.1 It has been used to monitor fructose uptake by the GLUT5 transporter in MCF-7, MDA-MB-435, and MDA-MB-231 human breast cancer cells. NBD-Fructose displays excitation/emission maxima of 472/538 nm, respectively.
References
1. Levi, J., Cheng, Z., Gheysens, O., et al. Fluorescent fructose derivatives from imaging breast cancer cells. Bioconjug.Chem. 18(3), 628-634 (2007).
| Cas No. | 940961-04-6 | SDF | |
| 别名 | 1-NBDF, 1-(7-nitro-1,2,3-benzadiazole)-Fructose | ||
| Canonical SMILES | O=C([C@@H](O)[C@H](O)[C@H](O)CO)CNC1=CC=C([N+]([O-])=O)C2=NON=C21 | ||
| 分子式 | C12H14N4O8 | 分子量 | 342.3 |
| 溶解度 | PBS (pH 7.2): 30 mg/ml,Water: 30 mg/ml | 储存条件 | Store at -20°C; protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9214 mL | 14.6071 mL | 29.2141 mL |
| 5 mM | 0.5843 mL | 2.9214 mL | 5.8428 mL |
| 10 mM | 0.2921 mL | 1.4607 mL | 2.9214 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Genetic evidence that uptake of the fluorescent analog 2NBDG occurs independently of known glucose transporters
PLoS One 2022 Aug 24;17(8):e0261801.PMID:36001583DOI:PMC9401136
The fluorescent derivative of glucose, 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)-amino]-D-glucose (2NBDG), is a widely used surrogate reagent to visualize glucose uptake in live cells at single cell resolution. Using CRISPR-Cas9 gene editing in 5TGM1 myeloma cells, we demonstrate that ablation of the glucose transporter gene Slc2a1 abrogates radioactive glucose uptake but has no effect on the magnitude or kinetics of 2NBDG import. Extracellular 2NBDG, but not NBD-Fructose was transported by primary plasma cells into the cytoplasm suggesting a specific mechanism that is unlinked from glucose import and that of chemically similar compounds. Neither excess glucose nor pharmacological inhibition of GLUT1 impacted 2NBDG uptake in myeloma cells or primary splenocytes. Genetic ablation of other expressed hexose transporters individually or in combination with one another also had no impact on 2NBDG uptake. Ablation of the genes in the Slc29 and Slc35 families of nucleoside and nucleoside sugar transporters also failed to impact 2NBDG import. Thus, cellular uptake of 2NBDG is not necessarily a faithful indicator of glucose transport and is promoted by an unknown mechanism.