Fluopyram
(Synonyms: 氟吡菌酰胺) 目录号 : GC34587
Fluopyram是一种琥珀酸脱氢酶(succinatedehydrogenase)抑制剂，为有效的杀真菌剂，能够抑制F.virguliforme的生长，平均EC50值为3.35µg/mL。
Cas No.:658066-35-4
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fluopyram is a succinate dehydrogenase inhibitor fungicide, inhibits the growth of F. virguliforme isolates with mean EC50 of 3.35 µg/mL[1]. Succinate dehydrogenase, Fungal[1]
[1]. Wang J, et al. Baseline Sensitivity of Fusarium virguliforme to Fluopyram Fungicide. Plant Dis. 2017 Apr;101(4):576-582.
| Cas No. | 658066-35-4 | SDF | |
| 别名 | 氟吡菌酰胺 | ||
| Canonical SMILES | FC(F)(F)C1=CC(Cl)=C(CCNC(C2=C(C(F)(F)F)C=CC=C2)=O)N=C1 | ||
| 分子式 | C16H11ClF6N2O | 分子量 | 396.71 |
| 溶解度 | DMSO : 125 mg/mL (315.09 mM);Water : < 0.1 mg/mL (insoluble) | 储存条件 | 4°C, away from moisture and light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.5207 mL | 12.6037 mL | 25.2073 mL |
| 5 mM | 0.5041 mL | 2.5207 mL | 5.0415 mL |
| 10 mM | 0.2521 mL | 1.2604 mL | 2.5207 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Fluopyram activates systemic resistance in soybean
Front Plant Sci 2022 Oct 24;13:1020167.PMID:36352871DOI:10.3389/fpls.2022.1020167.
The soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is a significant yield-limiting factor in soybean production in the Midwestern US. Several management practices are implemented to mitigate yield losses caused by SCN, including using SDHI (succinate dehydrogenase inhibitors) fungicides delivered as seed treatments. A set of studies was conducted to evaluate the effect of two seed-applied succinate dehydrogenase inhibitors (SDHI) compounds, Fluopyram and pydiflumetofen, on SCN population densities, plant injury, and plant growth. Cyst counts in untreated control and pydiflumetofen treated plants were 3.44 and 3.59 times higher than Fluopyram, respectively, while egg counts were 8.25 and 7.06 times higher in control and pydiflumetofen. Next-generation sequencing was later employed to identify transcriptomic shifts in gene expression profiles in Fluopyram and pydiflumetofen -treated seedlings. RNA expression patterns of seed treatments clustered by sampling time (5 DAP vs. 10 DAP); therefore, downstream analysis was conducted by timepoint. At 5 DAP, 10,870 and 325 differentially expressed genes (DEG) were identified in Fluopyram and pydiflumetofen, respectively. These same treatments generated 219 and 2 DEGs at 10 DAP. Multiple DEGs identified in soybean seedlings treated with Fluopyram are linked to systemic resistance, suggesting a potential role of systemic resistance in the suppression of SCN by Fluopyram, in addition to the known nematicidal activity. The non-target inhibition of soybean succinate dehydrogenase genes by Fluopyram may be the origin of the phytotoxicity symptoms observed and potentially the source of the systemic resistance activation reported in the current study. This work helps to elucidate the mechanisms of suppression of SCN by Fluopyram.
Mode of action of Fluopyram in plant-parasitic nematodes
Sci Rep 2022 Jul 13;12(1):11954.PMID:35831379DOI:10.1038/s41598-022-15782-7.
Plant-parasitic nematodes (PPN) are responsible for severe yield losses in crop production. Management is challenging as effective and safe means are rare. Recently, it has been discovered that the succinate dehydrogenase (SDH) inhibitor Fluopyram is highly effective against PPN while accompanying an excellent safety profile. Here we show that Fluopyram is a potent inhibitor of SDH in nematodes but not in mammals, insects and earthworm, explaining the selectivity on molecular level. As a consequence of SDH inhibition, Fluopyram impairs ATP generation and causes paralysis in PPN and Caenorhabditis elegans. Interestingly, efficacy differences of Fluopyram amongst PPN species can be observed. Permanent exposure to micromolar to nanomolar amounts of Fluopyram prevents Meloidogyne spp. and Heterodera schachtii infection and their development at the root. Preincubation of Meloidogyne incognita J2 with Fluopyram followed by a recovery period effectively reduces gall formation. However, the same procedure does not inhibit H. schachtii infection and development. Sequence comparison of sites relevant for ligand binding identified amino acid differences in SDHC which likely mediate selectivity, coincidently revealing a unique amino acid difference within SDHC conserved among Heterodera spp. Docking and C. elegans mutant studies suggest that this minute difference mediates altered sensitivity of H. schachtii towards Fluopyram.
Fluopyram removal from agricultural equipment rinsing water using HSF pilot-scale constructed wetlands
Environ Sci Pollut Res Int 2022 Apr;29(20):29584-29596.PMID:34519984DOI:10.1007/s11356-021-15373-5.
Fluopyram is a novel broad-spectrum fungicide with nematocidal activity, and as an extensively used pesticide, it could cause toxicity in nontarget organisms. The aim of this study was to explore the efficiency of five horizontal subsurface flow (HSF) constructed wetlands (CWs) to remove Fluopyram from rinsing water produced during the cleaning of pesticide spraying equipment. Four CWs, namely WG-R, WG-R-P, WG-C, and WG-U, contained fine gravel as porous media. WG-R and WG-R-P were planted with Phragmites australis, WG-C with Typha latifolia, and WG-U was left unplanted. Bioaugmentation with plant growth-promoting rhizobacteria was conducted in WG-R-P unit. The fifth unit (WGZ-R) planted with Phragmites australis and contained gravel and zeolite as porous media. All of CWs were loaded on a daily basis from December 2019 to January 2021 with water fortified with Fluopyram. The removal rate follows the pattern of WG-R-P (70.67%) > WGZ-R (62.06%) > WG-C (59.98%) > WG-R (36.10%) > WG-U (25.09%). The most important parameters affecting the Fluopyram removal were bioaugmentation, zeolite presence in porous media, and plant species. The WG-R-P unit showed higher Fluopyram removal in comparison to the WG-R (increase about 96%), the zeolite increased the Fluopyram removal by 72%, and the WG-C unit showed 66% higher Fluopyram removal than the WG-R unit.
Residue and Risk Assessment of Fluopyram in Carrot Tissues
Molecules 2022 Aug 29;27(17):5544.PMID:36080310DOI:10.3390/molecules27175544.
This study describes the variation in residue behavior of Fluopyram in soil, carrot root, and carrot leaf samples after the application of Fluopyram (41.7% suspension, Bayer) by foliar spray or root irrigation at the standard of 250.00 g active ingredient per hectare (a.i./ha) and double-dose treatment (500.00 g a.i./ha). Fluopyram and its metabolite fluopyram-benzamide were extracted and cleaned up using the QuEChERS method and subsequently quantified with LC-QQQ-MS/MS. The LOD and LOQ of the developed method were in the range of 0.05-2.65 ug/kg and 0.16-8.82 ug/kg, respectively. After root irrigation, the final residues detected in edible parts were 0.60 and 1.80 mg/kg, respectively, when 250.00 and 500.00 g a.i./ha were applied, which is much higher than the maximum residue limit in China (0.40 mg/kg). In contrast, after spray application, most of the Fluopyram dissipated from the surface of carrot leaves, and the final residues in carrot roots were both only 0.05 mg/kg. Dietary risk assessments revealed a 23-40% risk quotient for the root irrigation method, which was higher than that for the foliar spray method (8-14%). This is the first report comparing the residue behavior of Fluopyram applied by root irrigation and foliar spray. This study demonstrates the difference in risk associated with the two application methods and can serve as a reference for the safe application of Fluopyram.
Evaluation of Fluopyram for the control of Ditylenchus dipsaci in sugar beet
J Nematol 2020;52:1-10.PMID:32722906DOI:10.21307/jofnem-2020-071.
Fluopyram, a succinate dehydrogenase inhibitor fungicide, has shown potential in controlling Meloidogyne incognita and Rotylenchus reniformis in tomato. The effectiveness of this compound for the control of Ditylenchus dipsaci in sugar beet was evaluated. In this study, laboratory, growth chamber, glasshouse, and field experiments were conducted. In a motility bioassay, the EC50 value was determined with 3.00 μg/ml a.i. after 72 h exposure to Fluopyram. The growth chamber experiment did not show any effects on D. dipsaci penetration rate; however, field experiments revealed a positive effect of Fluopyram applied at planting in reducing D. dipsaci infectivity. The glasshouse experiment confirmed a limited effect of Fluopyram on D. dipsaci population development. Under field conditions, despite a reduction of D. dipsaci penetration rates in spring, Fluopyram was not effective in reducing the population development until harvest. Consequently, D. dipsaci densities in plant tissue and soil were high at harvest and not different among treatments. However, root-rot symptoms were significantly reduced at harvest. Fluopyram applied at planting showed good potential to reduce root-rot symptoms caused by D. dipsaci in sugar beet. However, for the long-term reduction of nematode populations in soil, further integrated control measures are needed to reduce the risks of substantial yield losses by D. dipsaci. Fluopyram, a succinate dehydrogenase inhibitor fungicide, has shown potential in controlling Meloidogyne incognita and Rotylenchus reniformis in tomato. The effectiveness of this compound for the control of Ditylenchus dipsaci in sugar beet was evaluated. In this study, laboratory, growth chamber, glasshouse, and field experiments were conducted. In a motility bioassay, the EC50 value was determined with 3.00 μg/ml a.i. after 72 h exposure to Fluopyram. The growth chamber experiment did not show any effects on D. dipsaci penetration rate; however, field experiments revealed a positive effect of Fluopyram applied at planting in reducing D. dipsaci infectivity. The glasshouse experiment confirmed a limited effect of Fluopyram on D. dipsaci population development. Under field conditions, despite a reduction of D. dipsaci penetration rates in spring, Fluopyram was not effective in reducing the population development until harvest. Consequently, D. dipsaci densities in plant tissue and soil were high at harvest and not different among treatments. However, root-rot symptoms were significantly reduced at harvest. Fluopyram applied at planting showed good potential to reduce root-rot symptoms caused by D. dipsaci in sugar beet. However, for the long-term reduction of nematode populations in soil, further integrated control measures are needed to reduce the risks of substantial yield losses by D. dipsaci.