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FGF 2 Human, Plant

目录号 : GP20214

Fibroblast Growth Factor-Basic Human Recombinant, Plant

FGF 2 Human, Plant Chemical Structure

规格 价格 库存 购买数量
10μg
¥840.00
5-10工作日
50μg
¥2,030.00
5-10工作日
1mg
¥17,640.00
5-10工作日

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Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

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产品描述

FGF-2 Human Recombinant produced in rice is a single, non-glycosylated polypeptide chain containing 146 amino acids and having a molecular mass of ~17kDa.The FGF-b protein is purified by proprietary chromatographic techniques.

Product Data

Purity Greater than 95.0% as determined by SDS-PAGE. Source Rice Grain (Oryza Sativa).
Phycical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Shipping Condition Shipped at Room temp.
Synonyms Prostatropin; HBGH-2; HBGF-2; FGF-2; FGF-b; Fibroblast growth factor 2; Basic fibroblast growth factor; Heparin-binding growth factor 2.
Solubility It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity The ED50, as calculated by the dose-dependent proliferation of Balb/c 3T3 cells expressing FGF receptors is <1 ng/ml, corresponding to a specific activity of >1 x106 Units/mg.
Formulation FGF-b was lyophilized from a concentrated solution without any additives.

Introduction

Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Biological Activity

The ED50, as calculated by the dose-dependent proliferation of Balb/c 3T3 cells expressing FGF receptors is <1 ng/ml, corresponding to a specific activity of >1 x106 Units/mg.

Stability

Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C . Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C .For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.