Chlortetracycline hydrochloride
(Synonyms: 盐酸金霉素; 7-Chlorotetracycline hydrochloride) 目录号 : GC60702
A tetracycline antibiotic
Cas No.:64-72-2
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Chlortetracycline is a broad-spectrum antibiotic originally isolated from S. aureofaciens.1,2 It inhibits growth of both Gram-positive and Gram-negative bacteria at a range of 0.1-100 μg/ml against A. aerogenes, D. pneumoniae, E. coli, K. pneumoniae, P. morganii, and several species of Haemophilus, Neisseria, Salmonella, and Staphylococcus.2 Chlortetracycline protects mice from infection by various strains of S. aureus with protective doses (PD50s) of 0.2-7.5 mg/kg, and from infection by E. coli (PD50 = 3 mg/kg) and K. pneumoniae (PD50 = 75 mg/kg).3 It acts by inhibiting protein synthesis, and it binds to a single site on the 30S ribosome subunit.1 Chlortetracycline is an ionophore and is selective for calcium over sodium, potassium, magnesium, strontium, and barium.4 It transports calcium from an aqueous phase into an organic phase environment or into multilamellar vesicles. Chlortetracycline is also a fluorescent dye that can be used to monitor calcium flux.5
1.Chopra, I., Hawkey, P.M., and Hinton, M.Tetracyclines, molecular and clinical aspectsJ. Antimicrob. Chemother.29(3)245-277(1992) 2.Paine, T.F., Jr., Collins, H.S., and Finland, M.Bacteriologic studies on aureomycinJ. Bacteriol.56(4)489-497(1948) 3.Cacciapuoti, A., Moss, E., Jr., Menzel, F., Jr., et al.In vitro and in vivo characterization of novel 8-methoxy derivatives of chlortetracyclineJ. Antibiot. (Tokyo)40(10)1426-1430(1987) 4.White, J.R., and Pearce, F.L.Characterization of chlortetracycline (aureomycin) as a calcium ionophoreBiochemistry21(24)6309-6312(1982) 5.Cerella, C., Mearelli, C., De Nicola, M., et al.Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracyclineAnn. N. Y. Acad. Sci.1099490-493(2007)
| Cas No. | 64-72-2 | SDF | |
| 别名 | 盐酸金霉素; 7-Chlorotetracycline hydrochloride | ||
| Canonical SMILES | O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@]3([H])[C@](C)(O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4Cl)N.[H]Cl | ||
| 分子式 | C22H24Cl2N2O8 | 分子量 | 515.34 |
| 溶解度 | ≥ 25.8 mg/mL in DMSO with gentle warming, ≥ 12.88 mg/mL in Water with ultrasonic and warming | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9405 mL | 9.7023 mL | 19.4047 mL |
| 5 mM | 0.3881 mL | 1.9405 mL | 3.8809 mL |
| 10 mM | 0.194 mL | 0.9702 mL | 1.9405 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Chlortetracycline hydrochloride removal by different biochar/Fe composites: A comparative study
J Hazard Mater 2021 Feb 5;403:123889.PMID:33264955DOI:10.1016/j.jhazmat.2020.123889.
In the last years, the synthesis and applications of biochar/Fe composites have been extensively studied, but only few papers have systematically evaluated their removal performances. Herein, we successfully synthesized and structurally characterized Fe0, Fe3C, and Fe3O4-coated biochars (BCs) for the removal of Chlortetracycline hydrochloride (CH). Evaluation of their removal rate and affinity revealed that Fe0@BC could achieve better and faster CH removal and degradation than Fe3C@BC and Fe3O4@BC. The removal rate was controlled by the O-Fe content and solution pH after the reaction. The CH adsorption occurred on the O C groups of Fe0@BC and the OC and OFe groups of Fe3C@BC and Fe3O4@BC. Electron paramagnetic resonance analysis and radical quenching experiments indicated that HO and 1O2/ O2- were mainly responsible for CH degradation by biochar/Fe composites. Additional parameters, such as effects of initial concentrations and coexisting anions, regeneration capacity, cost and actual wastewater treatment were also explored. Principal component analysis was applied for a comprehensive and quantitative assessment of the three materials, indicating Fe0@BC is the most beneficial functional material for CH removal.
Degradation of Chlortetracycline hydrochloride by peroxymonosulfate activation on natural manganese sand through response surface methodology
Environ Sci Pollut Res Int 2022 Nov;29(54):82584-82599.PMID:35752673DOI:10.1007/s11356-022-21556-5.
This work studies the degradation of Chlortetracycline hydrochloride (CTC) by activated peroxymonosulfate (PMS) with natural manganese sand (NMS). Meanwhile, the NMS was characterized and analyzed by isothermal nitrogen adsorption (BET), energy-dispersive X-ray spectroscopy (EDS) and scanning electron microscope (SEM). It can be induced that NMS material may contain C, O, Al, Si, Fe, Mn, and K, and the proportion of each is 6%, 9%, 13%, 34%, 27%, 5%, and 6%. Critical parameters, including initial pH value, catalyst dosage, and PMS amount, were optimized through response surface methodology. One of the essential significances of response surface methodology (RSM) is the establishment and optimization of the mathematical model to reduce the complexity of the experimental process. It can provide the degree of mutual influence between various factors and optimize the response based on the investigated factors. Results indicated that 81.65% of CTC could be degraded under the optimized conditions of PMS amount 2.02 g/L, the NMS dosage 0.29 g/L and pH 3.87. Also, it shows that NMS is the most powerful of each factor on the degradation efficiency. We proposed the degradation pathways of CTC from the liquid chromatograph-mass spectrometer (LC-MS) results. Therefore, NMS could be applied as an efficient activator of peroxymonosulfate to purify the water and wastewater.
Photocatalytic degradation of Chlortetracycline hydrochloride in marine aquaculture wastewater under visible light irradiation with CuO/ZnO
Water Sci Technol 2019 Oct;80(7):1249-1256.PMID:31850876DOI:10.2166/wst.2019.372.
A CuO/ZnO photocatalyst nanocomposite was successfully prepared by co-precipitation and characterized by investigating its chemical and physical properties by X-ray diffraction, scanning electron microscopy, energy dispersive spectroscopy, UV-vis diffuse reflectance spectroscopy and photoluminescence spectroscopy. The average particle size of CuO/ZnO composite was found to be around 80 nm. The degradation of Chlortetracycline hydrochloride pollutants in marine aquaculture wastewater using ZnO and CuO/ZnO was compared and it was found that CuO/ZnO nanocomposite is more efficient than ZnO. The effects of external factors on the photocatalytic effectiveness of nanocomposite were investigated under visible light. Also, the photocatalytic conditions for the degradation of Chlortetracycline hydrochloride by the nanocomposite were optimized. Based on both ability and efficiency of degradation, and on the cost and availability, 10:2 molar ratio of Zn2+/Cu2+ and 0.7 g/L nanocomposite, was found to be optimal, in which case the average photocatalytic degradation rate of Chlortetracycline hydrochloride reached 91.10%.
Detection of Chlortetracycline hydrochloride in Milk with a Solid SERS Substrate Based on Self-assembled Gold Nanobipyramids
Anal Sci 2020 Aug 10;36(8):935-940.PMID:32009022DOI:10.2116/analsci.19P476.
This paper described how a high-yield, monodisperse Au nanobipyramids (Au NBs) sol was prepared by a seed-mediated method, and gold nanoparticles were assembled on the surface of a silicon wafer by self-assembly technology to obtain a solid SERS substrate. Scanning electron microscopy (SEM) showed that the average length of Au NBs was 34.31 nm, and the analysis enhancement factor (AEF) was approximately 7.3 × 105 with rhodamine 6G (R6G) used as a probe. SERS detection of Chlortetracycline hydrochloride (CCH) in milk was performed utilizing the prepared Au NBs substrate, and the limit of detection was 0.01 mg/mL. In the range of 0.01 - 1 mg/mL, the mass concentration of CCH and the SERS signal intensity satisfied the linear relationship y = 258.467x + 150.501; the value of the correlation coefficient was 0.9785. In addition, the recovery of spiked samples fluctuated between 96.80 to 111.38%. These results proved that the method is simple and fast, and it is promising to be applied to the field detection of antibiotics in milk.
Nitrogen-doped carbon dots from rhizobium as fluorescence probes for Chlortetracycline hydrochloride
Nanotechnology 2020 Oct 30;31(44):445501.PMID:32688347DOI:10.1088/1361-6528/aba787.
Fluorescent nitrogen-doped carbon dots (CDs) were prepared via hydrothermal method at 190 °C for 10 h using rhizobium from soy as the carbon and nitrogen source. Their optical properties, structure, morphology, and functional groups were characterized in detail and the results showed that they possess unique excitation-dependent fluorescence behavior, with average diameter 4.5 ± 2.0 nm and good water dispersibility. Due to the overlap of the UV-vis absorbance of Chlortetracycline hydrochloride (CCH) and the fluorescence excitation band of CDs, the fluorescence of the prepared CDs can be quenched by CCH selectively and sensitively. The changes of the fluorescence intensity of CDs have a good linear relationship with the concentration of CCH in a wide concentration range of 5-100 μM, with a detection limit of 0.254 μM. This present method has been successfully applied to determine the CCH in water with recovery ranging from 96.0% to 100.7%.