CES1D Mouse
目录号 : GP21478Carboxylesterase 1D Mouse Recombinant
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greaterthan 90.0% as determined by SDS-PAGE. | Source | Sf9,Baculovirus cells. |
| Phycical Appearance | Sterile Filtered colorless solution. | Shipping Condition | Shipped with Ice Packs. |
| Synonyms | Carboxylesterase 1D; Carboxylesterase 3 (EC:3.1.1.1; EC:3.1.1.67); Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH; CES1D. | ||
| Amino Acid Sequence | YPSSPPVVNT VKGKVLGKYV NLEGFTQPVA VFLGVPFAKP PLGSLRFAPP QPAEPWSFVK NTTSYPPMCS QDAVGGQVLS ELFTNRKENI PLQFSEDCLY LNIYTPADLT KNSRLPVMVW IHGGGLVVGG ASTYDGLALS AHENVVVVTI QYRLGIWGFF STGDEHSRGN WGHLDQVAAL | ||
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. | ||
| Biological Activity | Specific activity is > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C. | ||
| Formulation | CES1D protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. | ||
Carboxylesterase 1D, also known as CES1Dis part of a big family of carboxylesterases which are responsible for thehydrolysis of ester in addition to amide bonds. CES1D is the principle lipase ofwhite adipose tissue fat cake extracts. Partially purified white adipose tissueCes1d had lipase activity in addition to lesser but detectable neutralcholesteryl ester hydrolase activity. CES1D demonstrates low catalyticefficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancertherapeutics.
Specific activity is > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.