Cell Counting Kit-8 (CCK-8)
目录号 : GK10001细胞计数试剂盒-8(CCK-8)是一种即用型的单瓶溶液,可快速、可靠地测量各种化学物质对细胞存活率和毒性的敏感度。
Sample solution is provided at 25 µL, 10mM.
- Adv Mater (2024):2401538.PMID:38738793
- Adv Mater (2023):2307193.PMID:37951210
- Adv Mater (2025):2413968.PMID:40223457
- Nat Microbiol (2025):1-16.PMID:39825096
- Cell Host Microbe 32.12 (2024):2195-2211.PMID:39610253
- Brain Behav Immun 110 (2023):60-79.PMID:36754245
- Adv Sci 7.9 (2020):1902938.PMID:32382476
- Nat Commun 15.1 (2024):72.PMID:38167723
- ACS nano 17.7 (2023):7017-7034.PMID:36971310
- Nat Commun 15.1 (2024):3771.PMID:38704377
- Nat Commun 14.1 (2023):3877.PMID:37391428
- Matter (2025).
- Adv. Fiber Mater. (2025):1-17.
- Acs Nano (2024).PMID:39361472
- ACS nano (2025).PMID:39846120
- Autophagy 18.5 (2022):1049-1061.PMID:34517786
- Cancer Commun (2024).PMID:38512764
- Brain Behav Immun (2024).PMID:38565398
- Autophagy just-accepted (2024).PMID:39007910
- Adv Sci 10.31 (2023):2301120.PMID:37688357
- Cancer Commun 43.5 (2023):582-612.PMID:37005481
- Nat. Commun. 16.1 (2025):2551.
- Adv-Sci 10.27 (2023):2302640.PMID:37485650
- Adv Sci (2024):2401845.PMID:38757623
- Nat Commun 16.1 (2025):3605.PMID:40240371
- Adv Sci (2023):2301107.PMID:37097746
- Acta Pharm Sin B (2025).
- Microbiome 12.1 (2024):152.PMID:39152482
- Adv Sci (2025):2413936.PMID:40047320
- Adv Sci (2025):2417374.PMID:40213972
- Adv Sci (2024):2404365.PMID:39159143
- Sci Adv 10.10 (2024):eadg7380.PMID:38457499
- Adv Sci (2024):2402450.PMID:38952061
- Emerg Microbes Infec 12.1 (2023):2204164.PMID:37060263
- J Exp Clin Canc Res 41.1 (2022):293.PMID:36199122
- Nano Today 61 (2025):102560.
- Water Res (2024):121379.PMID:38422694
- Biomaterials (2025):123335.PMID:40222258
- Small 21.3 (2025):2409121.PMID:39544127
- J Med Virol 95.10 (2023):e29171.PMID:37830751
- J Adv Res (2023).PMID:36933884
- Small (2024):2407674.PMID:39363789
- Theranostics 12.7 (2022):3488.PMID:35547749
- Theranostics 14.16 (2024):6161.PMID:39431016
- Mol. Ther. 33.3 (2025):1134.PMID:39810420
- Small Methods (2024):2400223.PMID:38602202
- Mater Horiz (2024).PMID:39189308
- Mol Ther (2025).PMID:39810420
- Gut Microbes 16.1 (2024):2347757.PMID:38773738
- Cell Death Differ 30.10 (2023):2293-2308.PMID:37608075
- Gut Microbes 17.1 (2025):2446376.PMID:39718561
- J Hazard Mater (2024):135904.PMID:39303616
- Mater Horiz (2024).PMID:39189308
- J Exp Clin Canc Res 39 (2020):1-13.PMID:32028978
- Cell Discov 6.1 (2020):96.PMID:33349633
- Redox Biol 62 (2023):102663.PMID:36924682
- J Exp Clin Canc Res 44.1 (2025):1-19.PMID:40069844
- J Adv Res (2025).PMID:40373960
- Redox Biol (2023):102983.PMID:38064762
- Environ Sci Technol 56.21 (2022):15096-15107.PMID:36099323
- J Adv Res (2025).
- J Control Release 360 (2023):647-659.PMID:37406817
- Sci Total Environ 878 (2023):162942.PMID:36940749
- Environ Sci Technol (2024).PMID:39364562
- J Control Release 365 (2024):301-316.PMID:38007195
- Acta Biomater 157 (2023):321-336.PMID:36481504
- Clin Transl Med 14.1 (2024):e1539.PMID:38224186
- J Exp Clin Canc Res 43.1 (2024):187.PMID:38965580
- J Exp Clin Canc Res 43.1 (2024):91.PMID:38528516
- J Control Release 379 (2025):89-104.PMID:39756690
- J Exp Clin Canc Res 41.1 (2022):1-15.PMID:34980201
- J Immunother Cancer 12.3 (2024):e008054.PMID:38429070
- J Control Release 349 (2022):254-268.PMID:35803328
- EBioMedicine 99 (2024).PMID:38101299
- Cell Discov 6.1 (2020):96.PMID:33349633
- J Control Release 373 (2024):749-765.PMID:39084465
- J Exp Clin Canc Res 41 (2022):1-15.PMID:34980201
- J Control Release 359 (2023):12-25.PMID:37244298
- J Exp Clin Canc Res 41 (2022):1-15.PMID:34980201
- Water Res (2024):122403.PMID:39278116
- Rare Met. (2025):1-23.
- Adv Healthc Mater (2025):2500845.PMID:40400499
- J Nanobiotechnol 22.1 (2024):1-15.PMID:38790037
- Cancer Lett (2024):216765.PMID:38408604
- Adv Healthc Mater (2024):2401061.PMID:38849128
- J Nanobiotechnol 21.1 (2023):354.PMID:37775761
- Cardiovasc Res 121.2 (2025):324-338.PMID:39658102
- Adv Healthc Mater 13.8 (2024):2303215.PMID:38112062
- View-China (2025):20240103.
- Adv Healthc Mater 12.9 (2023):2202785.PMID:36541060
- J Immunother Cancer 12.10 (2024):e009416.PMID:39455095
- J Neuroinflamm 20.1 (2023):1-16.PMID:36944954
- J Nanobiotechnol 20.1 (2022):1-20.PMID:36123708
- Adv Healthc Mater (2024):2402877.PMID:39434433
- Pharmacol Res 187 (2023):106563.PMID:36410674
- Acta Biomater (2024).PMID:38452962
- Adv Healthc Mater 11.8 (2022):2102395.PMID:34874119
- Environ Health Persp 131.6 (2023):067004.PMID:37267060
- Sci Total Environ 897 (2023):165514.PMID:37451464
- J Nanobiotechnol 23.1 (2025):1-22.PMID:39871272
- ENGINEERING-PRC (2025).
- J Nanobiotechnol 22.1 (2024):1-21.PMID:38448959
- J Nanobiotechnol 20.1 (2022):1-20.PMID:36123708
- Food Chem (2024):139505.PMID:38703732
- Acs Appl Mater Inter (2024).PMID:38345297
- CANCER LETT (2025):217685.
- Mater. Today Bio (2025):101703.
- Brit J Cancer (2024):1-9.PMID:38594370
- J Colloid Interf Sci (2025):137652.
- Acta Biomater 113 (2020):393-406.PMID:32629189
- Cancer Lett 611 (2025):217418.PMID:39725146
- Pharmacol Res 180 (2022):106225.PMID:35452801
- Cell Mol Life Sci 80.3 (2023):61.PMID:36763212
- Cell Death Dis 13.5 (2022):497.PMID:35614040
- Liver Int 43.4 (2023):840-854.PMID:36737842
- Pharmacol Res (2024):107460.PMID:39393436
- Cell Mol Biol Lett 29.1 (2024):103.PMID:38997648
- J Colloid Interf Sci (2025):.PMID:39755016
- Biomark Res 11.1 (2023):3PMID:36611218
- Mol Ther-Nucl Acids 31 (2023):703-716.PMID:36923951
- Acta Biomater (2025).PMID:39952883
- Acs Appl Mater Inter 15.11 (2023).PMID:36897565
- Front Immunol 14 (2023):1130308.PMID:37006248
- Cell Death Dis 13.10 (2022):858.PMID:36209201
- Environ Pollut 334 (2023):122148.PMID:37419204
- Acs Appl Mater Inter 15.32 (2023):38294-38308.PMID:37542453
- Acta Biomater (2025).PMID:40024424
- Environ Pollut 338 (2023):122581.PMID:37748638
- Cell Mol Life Sci 79.7 (2022):382.PMID:35751755
- ACS Appl Mater Inter 14.13 (2022).PMID:35344348
- Pharmacol Res 195 (2023):106877.PMID:37524154
- Cancer Lett (2024):217418.PMID:39725146
- Biomater Sci-UK 10.17 (2022):5006-5017.PMID:35815806
- Int J Biol Macromol 220 (2022):371-384.PMID:35970372
- Int J Biol Macromol 224 (2023):797-809.PMID:36283555
- Cell Mol Life Sci 81.1 (2024):32.PMID:38214780
- Free Radical Bio Med 188 (2022):404-418.PMID:35787451
- Environ Res (2024):118221.PMID:38246300
- Free Radical Bio Med 190 (2022):202-215.PMID:35985562
- Phytomedicine 122 (2024):155091.PMID:37844378
- Cell Death Dis 12.8 (2021):779.PMID:34365463
- Int. J. Biol. Markers (2025):143683.PMID:40318724
- Phytomedicine 122 (2024):155091.PMID:37844378
- Front Immunol 16 (2025):1569210.PMID:40396173
- Int Immunopharmacol 158 (2025):114885.PMID:40383096
- Sci Rep-Uk 15.1 (2025):1-17.PMID:40383858
- Phytomedicine 118 (2023):154944.PMID:37393830
- Phytomedicine (2023):155270.PMID:38096717
- Int. J. Biol. Macromol. (2025):143759.
- Int J Biol Macromol(2023):125130.PMID:37263336
- INT J BIOL SCI 21.7 (2025):3099-3121.
- Oncogene (2023):1-13.PMID:37138031
- Int J Biol Macromol 222 (2022):1789-1800.PMID:36191790
- CANCER IMMUNOL RES (2025).PMID:40323348
- Int J Biol Macromol (2024):129172.PMID:38176496
- Antivir Res 205 (2022):105381.PMID:35835291
- Cell Death Dis 12.4 (2021):408.PMID:33859186
- Cell Death Dis 13.3 (2022):233.PMID:35288538
- Int J Biol Macromol (2024):135979.PMID:39332550
- Int J Biol Macromol (2024):131928.PMID:38688339
- Cell Death Dis 16.1 (2025):240.PMID:40175350
- Int J Biol Macromol (2024):135562.PMID:39276884
- Int J Biol Macromol 306 (2025):141410.PMID:40010477
- Int J Biol Sci 21.2 (2025):614.PMID:39781455
- Acs Appl Mater Inter (2025).PMID:40025657
- Sci China Life Sci (2025):1-11.PMID:39821161
- Int J Biol Macromol (2025):140602.PMID:39922352
- Acs Appl Mater Inter (2024).PMID:39448903
- Int J Biol Macromol (2025):141767.
- Acs Appl Mater Inter (2025).PMID:39853325
- Int. J. Biol. Macromol. (2025):142238.PMID:40112979
- Acs Appl Mater Inter (2024).PMID:39320506
- Int J of Bio Macromol (2024):132103.PMID:38719011
- Cell Mol Biol Lett 29.1 (2024):83.PMID:38822277
- Cell Commun Signal 22.1 (2024):293.PMID:38802896
- Int J Biol Macromol (2024):136818.PMID:39447809
- Int J Biol Macromol (2025):141146.PMID:39986528
- Nanoscale Horiz (2025).
- Mater Today Bio (2024):101019.PMID:38516170
- Int J Biol Macromol (2025):140632.PMID:39909240
- Int J Biol Macromol (2024):133667.PMID:38969038
- Int J Biol Macromol (2025):140374.PMID:39880265
- Int J Biol Sci 21.4 (2025):1837-1851.
- Cell Mol Life Sci 81.1 (2024):143.PMID:38493426
- Cell Commun Signal 22.1 (2024):1-19.PMID:38454413
- Int J Biol Macromol (2024):137561.PMID:39537068
- Cell Commun Signal 22.1 (2024):1-23.PMID:39696352
- Acs Sensors (2024).PMID:38958530
- Cell Death Dis 15.11 (2024):826.PMID:39543094
- Antioxid Redox Sign 37.13 (2022):867-886.PMID:35350885
- J Med Chem (2025).PMID:39842821
- Virol Sin (2023).PMID:37331658
- Biomed Pharmacother 122 (2020):109690.PMID:31786468
- Biomed Pharmacother 179 (2024):117415.PMID:39265236
- Biomed Pharmacother 130 (2020):110566.PMID:32755793
- Front Immunol 13 (2022):902515.PMID:35874682
- Phytomedicine (2024):155859.PMID:38972239
- J Mater Chem B 11.16 (2023):3740-3751.PMID:37067505
- Front Immunol 13 (2022):986214.PMID:36341437
- Bioeng Transl Med 8.3 (2023):e10475.PMID:37206234
- Front Immunol 14 (2023):1111494.PMID:36817451
- Eur J Med Chem 238 (2022):114458.PMID:35635946
- Fertil Steril (2023).PMID:37178109
- Free Radical Bio Med (2024).PMID:38367927
- Plos Pathog 17.3 (2021):e1009393.PMID:33684175
- J Med Chem 65.4 (2021):2794-2808.PMID:33872498
- Int J Nanomed (2025):4535-4550.PMID:4023652
- J Gastroenterol (2023):1-14.PMID:37150773
- Wirel Commun Mob Com 2 (2022):3.PMID:36120596
- Cell Biol Toxicol 38.6 (2022):1121-1136.PMID:35348966
- Front Nutr 9 (2022):812623.PMID:35419394
- Clin Epigenetics 14.1 (2022):1-12.PMID:35414117
- Int J Nanomed (2024):13183-13199.PMID:39664759
- Mater Today Bio 15 (2022):100264.PMID:35517578
- Oncogenesis 11.1 (2022):21.PMID:35487890
- Int J Biol Macromol 211 (2022):128-139.PMID:35561854
- Int J Biol Macromol 211 (2022):128-139.PMID:35561854
- J Med Virol 96.11 (2024):e70049.PMID:39558699
- Front Cell Dev Biol 10 (2022):850708.PMID:35592246
- Stem Cell Res Ther 15.1 (2024) 385.PMID:39468662
- Life Sci 308 (2022):120925.PMID:36057399
- Bba-Mol Basis Dis 1869.1 (2023):166571.PMID:36244542
- Bba Mol Basis Dis 1869.7 (2023):166725.PMID:37127173
- Food Chem Toxicol 148 (2021):111972.PMID:33421461
- Front Cell Dev Biol 9 (2021):749210.PMID:34676217
- Life Sci 312 (2023):121092.PMID:36279968
- J Cell Physiol 238.1 (2023):195-209.PMID:36436184
- Int J Nanomed 20 (2025):293-308.
- Biomed Pharmacother 158 (2023):114118.PMID:36527845
- Food Res Int (2025):115940.
- Oncogene (2025):1-15.PMID:39920289
- Front Cell Dev Biol 9 (2021):696558.PMID:34447749
- Biomed Pharmacother 129 (2020):110315.PMID:32554246
- Biomed Pharmacother 162 (2023):114602.PMID:37018993
- J Med Chem (2024).PMID:39292661
- Stem Cell Res Ther 15.1 (2024):1-18.PMID:39267160
- Ecotox Environ Safe 262 (2023):115161.PMID:37356398
- Ecotox Environ Safe 270 (2024):115813.PMID:38113798
- J Med Virol 97.3 (2025):e70267.
- Biomed Pharmacother 165 (2023):115261.PMID:37549461
- Phytomedicine (2025):156860.
- J. Environ. Chem. Eng. (2025):116286.
- Cell Rep 44.3 (2025).PMID:39977268
- Nanoscale 16.17 (2024):8479-8494.PMID:38590261
- Phytother Res 37.10 (2023):4639-4654.PMID:37394882
- Phytomedicine 130 (2024):155744.PMID:38763011
- Cell Death Discov 9.1 (2023):415.PMID:37968255
- Front Immunol 14 (2023).PMID:37662906
- Biomed Pharmacother 188 (2025):118217.PMID:40435839
- Phytother Res (2024).PMID:38558449
- Biomed Pharmacother 174 (2024):116470.PMID:38565061
- Stem Cell Res Ther 10.1 (2019):1-14.PMID:31847890
- Phytomedicine (2025):156598.
- Cell Death Discov 11.1 (2025):1-16.PMID:40399275
- Embo Rep (2025):1-23. PMID:40185977
- J Polym Environ (2025):1-16.
- Biomed Pharmacother 184 (2025):117907.PMID:39978030
- Phytomedicine (2025):156515.PMID:39983438
- J Med Chem (2024).PMID:38315032
- AGING DIS 17.1 (2026):2.PMID:40072367
- Npj Precis Oncol 9.1 (2025):112.PMID:40240582
- Iscience (2025).
- Biomed Pharmacother 177 (2024):117037.PMID:38959602
- Plos Pathog 19.10 (2023):e1011702.PMID:37801439
- Anal Chem 95.31 (2023):11664-11671.PMID:37495553
- Ecotox Environ Safe 280 (2024):116579.PMID:38865940
- Cell Death Discov 10.1 (2024):7.PMID:38182573
- Cell Death Discov 10.1 (2024):97.PMID:38402198
- Acta Pharmacol Sin (2025):1-15.PMID:39984622
- Biomed Pharmacother 168 (2023):115751.PMID:37879214
- Brit J Pharmacol (2024).PMID:38715438
- Stem Cell Res Ther 16.1 (2025):1-18.PMID:40361250
- Stem Cell Res Ther 16.1 (2025):119.PMID:40050973
- Ecotox Environ Safe 266 (2023):115571.PMID:37837696
- Bba-Mol Basis Dis (2024):167210.PMID:38704001
- Int J Mol Sci 24.22 (2023):16503.PMID:38003694
- Front Cell Dev Biol 9 (2022):793793.PMID:35155447
- Int J Biochem Cell B 153 (2022):106317.PMID:36283586
- J Agr Food Chem 70.50 (2022):15763-15775.PMID:36472370
- Apoptosis 28.3-4 (2023):414-431.PMID:36482019
- Exp Neurol 359 (2023):114266.PMID:36336032
- Biomater Adv (2024):214069.PMID:39447240
- J Agr Food Chem (2023).PMID:37922022
- Microbiol Res (2024):127927.PMID:39393129
- Colloid Surface B 222 (2023):113119.PMID:36621177
- Biomolecules 12.9 (2022):1283.PMID:36139122
- Int Immunopharmacol 113 (2022):109342.PMID:36327871
- Ecotox Environ Safe 287 (2024):117331.PMID:39547060
- Life Sci (2024):122483.PMID:38307238
- Biochem Pharmacol (2024):116044.PMID:38336157
- Front Oncol 12 (2022):770628.PMID:35372056
- Acs Biomater Sci Eng (2024).PMID:38445959
- Acs Biomater Sci Eng (2024).PMID:38446137
- Am J Chinese Med (2024):1-23.PMID:38490807
- Mol Med 30.1 (2024):14.PMID:38254010
- Ecotox Environ Safe 289 (2025):117511.PMID:39662457
- Am J Physiol-Cell Ph (2024).PMID:38525543
- J Enzym Inhib Med Ch 39.1 (2024):2288810.PMID:38059334
- Int J Biochem Cell B 153 (2022):106317.PMID:36283586
- Front Immunol 15 (2024):1455996.PMID:39376562
- Mol Neurobiol 59.12 (2022):7075-7094.PMID:36083519
- Int Immunopharmacol 131 (2024):111922.PMID:38522137
- Front Oncol 12 (2022):858598.PMID:35321435
- Cancer Cell Int 24.1 (2024):123.PMID:38566075
- Front Bioeng Biotech 12 (2024):1361682.PMID:38562665
- Eur J Pharm Biopharm 188 (2023):89-99.PMID:36702198
- Front Immunol 15 (2024):1455996.PMID:39376562
- Int Immunopharmacol 132 (2024):111870.PMID:38547771
- Front Immunol 15 (2024):1375781.PMID:39391314
- Int J Mol Med 54.6 (2024):1-17.PMID:39364745
- Front Oncol 13 (2023):1120103.PMID:36959804
- J Agr Food Chem 71.3 (2023):1593-1606.PMID:36634077
- Front Oncol 12 (2022):858598.PMID:35321435
- J Mater Chem B (2024).PMID:39119756
- Cells 11.23 (2022):3816.PMID:36497076
- Aging (Albany NY) 16.11 (2024):9625.PMID:38829771
- J Transl Med 22.1 (2024):1-19.PMID:39107788
- Front Cell Dev Biol 10 (2022):787730.PMID:35445026
- Molecular Therapy Oncology 32.2 (2024).PMID:38948919
- Front Pharmacol 13 (2022):819826.PMID:35645824
- Front Pharmacol 13 (2022):972397.PMID:36188529
- Apoptosis (2024):1-19.PMID:39097858
- Front Pharmacol 13 (2022):1015693.PMID:36210855
- Cell Mol Life Sci 81.1 (2024):265.PMID:38880863
- Front Pharmacol 14 (2023):1168393.PMID:37124212
- Cns Neurosci Ther (2023).PMID:37452510
- Int J Pharmaceut (2023):123142.PMID:37328119
- Int J Mol Sci 23.24(2022):16045.PMID:36555684
- Cancer Cell Int 23.1 (2023):142.PMID:37468908
- Aging (Albany NY) 15.12 (2023):5673.PMID:37387540
- Lab Chip (2024).PMID:38939907
- Cells 11.24 (2022):4094.PMID:36552856
- Jci Insight (2024). PMID:38935435
- Int J Mol Sci 23.22 (2022):14306.PMID:36430782
- Mar Life Sci Tech 4.3 (2022):373-383.PMID:37073164
- Biochem Pharmacol (2023):115632.PMID:37263300
- Front Immunol 15 (2024):1455996.PMID:39376562
- J Transl Med 22.1 (2024):1-17.PMID:39267037
- Mbio (2023):e01688-23.PMID:37882780
- Front Immunol 15 (2024):1375781.PMID:39391314
- Chinese Med J-Peking (2023):10-1097.PMID:36939243
- Chinese Med J-Peking 136.05 (2023):556-564.PMID:36939243
- Front Pharmacol 13 (2022):946193.PMID:36091823
- Phytother Res (2023).PMID:37086359
- Faseb J 37.5 (2023):e22935.PMID:37086094
- Resp Res 24.1 (2023):216.PMID:37674165
- Mol Med 30.1 (2024):1-18.PMID:39266965
- Biochem Pharmacol 213 (2023):115592.PMID:37196680
- Jci insight 9.15 (2024):PMID:38935435
- Phytother Res (2023).PMID:37246830
- J Transl Med 22.1 (2024):1-15.PMID:39256832
- Front Oncol 13 (2023):1132424.PMID:37251940
- Int Immunopharmacol 121 (2023):110413.PMID:37301119
- Am J Cancer Res 13.5 (2023):2135.PMID:37293170
- Front Cell Dev Biol (2023):1161541.PMID:37325570
- Front Pharmacol 13 (2022):1043022.PMID:36588728
- Front Pharmacol 13 (2022):927488.PMID:35935870
- Int-Immunopharmacol 124 (2023):110809.PMID:37690240
- J Nutr Biochem (2024):109678.PMID:38844080
- Front Pharmacol 13 (2022):965131.PMID:36249790
- Stem Cell Transl Med (2024):szae020.PMID:38530131
- Cancer Cell Int 20 (2020):1-14.PMID:33292221
- Cancer Cell Int 21 (2021):1-12.PMID:34281530
- Mol Cell Proteomics (2025):100930.PMID:40024376
- Apoptosis (2025):1-17.PMID:40347321
- Phytother. Res. 39.3 (2025):1355-1371.PMID:39764683
- Aging (Albany NY) 13.17 (2021):21712.PMID:34518442
- Int J Mol Sci 22.21 (2021):11893.PMID:34769322
- Talanta (2025):127591.PMID:39847915
- MOL MED 31.1 (2025):126.PMID:40186145
- Front Immunol 16 (2025):1569210.
- Lab Invest 102.4 (2022):411-421.PMID:34775495
- Int J Mol Med 55.3 (2025):1-14.PMID:39791203
- Mol Med 31.1 (2025):1-15.PMID:40369420
- Liver Int 45.2 (2025):e16246.PMID:39821525
- Front Immunol 16 (2025):1521965.PMID:40191190
- Front Pharmacol 12 (2021):649398.PMID:34335241
- Biomacromolecules (2025).
- J Agr Food Chem (2025).PMID:40017402
- J Invest Dermatol (2025).PMID:39892773
- Mol Neurobiol 58 (2021):1990-2004.PMID:33411316
- Aging (Albany NY) 13.3 (2021):4482.PMID:33495411
- Front Pharmacol 12 (2021):610158.PMID:33762939
- J Agr Food Chem 71.1 (2022):546-556.PMID:36538589
- Cancer Gene Ther (2021)1-12.PMID:33888871
- J Transl Med 23.1 (2025):131.PMID:39885513
- Int J Mol Med 55.3 (2025):1-17.
- Front Pharmacol 11 (2020):16.PMID:32140106
- Front Pharmacol 12 (2021):673263.PMID:34108880
- Front. Immunol. 16 (2025):1556715.
- Int J Mol Med 55.3 (2025):45.PMID:39791203
- J Agr Food Chem (2025).PMID:40152883
- Int J Mol Med 55.4 (2025):1-16.PMID:39918010
- Mol Med 31.1 (2025):1-15.PMID:40405066
- BREAST CANCER RES 27.1 (2025):61.PMID:40270028
- J Agr Food Chem (2025).PMID:39962827
- Cancer Gene Ther 29.8-9 (2022):1228-1239.PMID:35115683
- Ecotox Environ Safe 233 (2022):113315.PMID:35189521
- Biomater Adv (2025):214257.PMID:40023084
- Cell Death Discov 11.1 (2025):74.PMID:39988672
- Mol Med 30 (2024):260.PMID:39707194
- Microbiol Res (2025):128183.
- J Invest Dermatol (2025).PMID:39892773
- Pharmaceuticals 15.11 (2022):1435.PMID:36422564
- Mol Carcinogen (2023).PMID:37750597
- Antimicrob Agents Ch 65.6 (2021):10-1128.PMID:33753340
- Pharmaceuticals 15.10 (2022):1271.PMID:36297383
- J Ethnopharmacol (2024):117985.PMID:38417600
- J Ethnopharmacol (2023):116731.PMID:37277084
- J Virol (2023):e00549-23.PMID:37222617
- Am J Cancer Res 14.1 (2024):324.PMID:38323285
- Eur-J Pharmacol 958 (2023):176033.PMID:37673365
- Am J Physiol-Gastr L (2024).PMID:38742280
- J Neurochem 164.5 (2023):643-657.PMID:36527420
- Transl Oncol 47 (2024):102042.PMID:38924847
- J Ethnopharmacol 307 (2023):116193.PMID:36746295
- Molecules 28.6 (2023):2426.PMID:36985397
- Molecules 27.23 (2022):8568.PMID:36500666
- Food Funct. (2025).
- Nutrients 17.5 (2025):923.PMID:40077793
- Int Immunopharmacol 138 (2024):112559.PMID:38955028
- Life Sci (2024):123148.PMID:39447733
- Sci Rep-Uk 13.1 (2023):14339.PMID:37658227
- Molecules 27.17 (2022):5698.PMID:36080465
- Radiother Oncol 203 (2025):110686.PMID:39709027
- Int Immunopharmacol 141 (2024):112955.PMID:39163685
- Bioorg Chem 136 (2023):106556.PMID:37105002
- Invest Ophth Vis Sci 66.1 (2025):24-24.PMID:39792075
- Aging (Albany NY) 16.8 (2024):6773.PMID:38643468
- Sci Rep-Uk 13.1 (2023):361.PMID:36611103
- J Cell Mol Med 24.7 (2020):4136-4149.PMID:32100443
- Aging 16.8 (2024):6773-6795.PMID:38643468
- Molecules 27.14 (2022):4603.PMID:35889477
- Clin Immunol (2024):110308.PMID:39002794
- Cell Death Discov 8.1 (2022):85.PMID:35220396
- J Agr Food Res (2025):101694.
- Mol Neurobiol 59.8 (2022):4776-4790.PMID:35622272
- Molecules 27.21 (2022):7183.PMID:36364008
- J-Ethnopharmacol (2023):116729.PMID:37277081
- Mol Nutr Food Res (2024):2300912.PMID:38847553
- Int Immunopharmacol 108 (2022):108742.PMID:35378447
- Exp Neurol (2025):115252.PMID:40221008
- Front Oncol 12 (2022):996080.PMID:36483054
- Environ Toxico (2024).PMID:38700384
- J Oncol 2022 (2022).PMID:36213838
- J Nutr Biochem (2024):109780.PMID:39395694
- Antimicrob Agents Ch 65.6 (2021):10-1128.PMID:33753340
- Diabetol Metab Syndr 16.1 (2024):1-14.PMID:38317244
- Chem-Biol Interact 382 (2023):110638.PMID:37473910
- Acs Appl Bio Mater (2024).PMID:38700956
- Int Immunopharmacol 138 (2024):112533.PMID:38924868
- J Drug Deliv Sci Tec 104 (2025) 106577.
- Life Sci 254 (2020):117793.PMID:32416164
- Front Endocrinol 13 (2022):998971.PMID:36147560
- J Dermatol Sci 109.2 (2023):52-60.PMID:36858850
- Mol Neurobiol (2025):1-16.PMID:40402409
- Bioengineering 9.9 (2022):433.PMID:36134979
- Bone Joint Res 13.12 (2024):763-777.PMID:39662502
- Acs Infect Dis 8.9 (2022):1851-1868.PMID:35948057
- J Ethnopharmacol (2024):118080.PMID:38521426
- Biotechnol J 19.1 (2024):2300453.PMID:37899497
- Transl Oncol 56 (2025):102396.PMID:40239242
- Int J Mol Sci 26.10 (2025):4953.PMID:40430095
- Int Immunopharmacol 159 (2025):114886.PMID:40424656
- Cns Neurosci Ther 31.5 (2025):e70437.PMID:40400263
- J Hepatocell Carcino (2023):863-881.PMID:37313303
- Int Immunopharmacol 138 (2024):112539.PMID:38936054
- Int J Mol Med 52.5 (2023):1-14.PMID:37800609
- Int Immunopharmacol 107 (2022):108695.PMID:35305385
- Front Cell Dev Biol 12 (2024):1391873.PMID:39170916
- J Ethnopharmacol (2024):119073.PMID:39522846
- J Ethnopharmacol (2024):118685.PMID:39127116
- Environ Toxicol (2023).PMID:38069587
- Int. J. Mol. Sci. 26.9 (2025):4325.
- J Ethnopharmacol (2024):119272.PMID:39716512
- Commun Biol 8.1 (2025):71.PMID:39820362
- Faseb J 38.7 (2024):e23591.PMID:38572579
- Burns Trauma 12 (2024):tkae001.PMID:38434722
- Glia (2024).PMID:39610064
- Front Oncol 12 (2022):1043161.PMID:36439418
- Aging (Albany NY) 15.23 (2023):14292.PMID:38059876
- Front Oncol 12 (2022):961653.PMID:36457504
- J. Drug Delivery Sci. Technol. (2025):106841.
- CANCER CELL INT 25.1 (2025):1-24.PMID:40296031
- J Dent (2024):105436.PMID:39488296
- Drug Des Dev Ther (2025):281-302.PMID:39845152
- Macromol Biosci (2024):2400003.PMID:38597147
- Int Immunopharmacol 138 (2024):112625.PMID:38996666
- J Ethnopharmacol (2024):117958.PMID:38395179
- J Cell Mol Med 26.13 (2022):3783-3796.PMID:35656815
- Invest Ophth Vis Sci 63.6 (2022):13-13.PMID:35695808
- Biochem Pharmacol (2024):116688.PMID:39617210
- Redox Rep 29.1 (2024):2398380.PMID:39284588
- J Ethnopharmacol 319 (2024):117091.PMID:37634753
- Toxicology (2024):154018.PMID:39608440
- Front Endocrinol 13 (2022):657802.PMID:36263320
- Radiother Oncol (2024):110686.PMID:39709027
- ACS Appl. Nano Mater. (2025).
- Cell Cycle 21.8 (2022):835-850.PMID:35100080
- J Ethnopharmacol 319 (2024):117205.PMID:37741473
- Cell Cycle 21.3 (2022):228-246.PMID:34965191
- J Oncol 2022 (2022):6459029PMID:36199788
- J oncol 2022 (2022).PMID:36385962
- J Oncol 2022 (2022).PMID:36281290
- Int Immunopharmacol 147 (2025):113871.PMID:39798467
- Am J Physiol-Cell Ph (2024).PMID:39069827
- Mol Neurobiol (2024):1-18.PMID:38995443
- Drug Deliv Transl Re (2023):1-13.PMID:37130996
- Cell Cycle 19.17 (2020):2139-2147.PMID:32779548
- Biochem Pharmacol (2024):116438.PMID:39025409
- Macromol Biosci (2024):2400003.PMID:38597147
- Transl Oncol 52 (2025):102256.PMID:39733744
- J Ethnopharmacol (2024):117865.PMID:38369066
- Mol. Nutr. Food Res. (2025):e70053.PMID:40177855
- Food Biosci (2025):106564.
- Int Immunopharmacol 138 (2024):112533.PMID:38924868
- J Dent (2024):105436.PMID:39488296
- Cell Signal 113 (2024):110962.PMID:37931691
- Arch Toxicol (2024):1-16.PMID:38971901
- Mol Carcinogen 62.2 (2023):160-173.PMID:36222184
- J Virol 97.11 (2023):e00719-23.PMID:37929962
- J Oncol 2022 (2022).PMID:36213831
- Acs Biomater Sci Eng (2024).PMID:39164198
- Bone Joint Res 13.12 (2024):763.PMID:39662502
- Microorganisms 11.5 (2023):1268.PMID:37317242
- J Cell Mol Med 28.6 (2024):e18223.PMID:38451046
- Colloid Surface B (2025):114537.PMID:39879672
- J. Ethnopharmacol. (2025):119727.PMID:40187623
- Cell Signal (2023):110987.PMID:38029946
- J Ethnopharmacol 310 (2023):116375.PMID:36934787
- J Cancer 15.5 (2024):1397.PMID:38356722
- Front Pharmacol 15 (2025):1534772.PMID:39911826
- J Neuro Oncol (2023):1-16.PMID:37351767
- Eur J Pharmacol (2024):176995.PMID:39277096
- Heliyon 8.11 (2022).PMID:36353164
- J Ethnopharmacol 259 (2020):112956.PMID:32442587
- Bmc Genomics 25.1 (2024):1-13.PMID:38395755
- Toxicol Lett 379 (2023):20-34.PMID:36905973
- Bmc Complement Med 24.1 (2024):1-16.PMID:38580999
- J Biochem Mol Toxic 38.1 (2024):e23544.PMID:37815058
- Oncotargets Ther (2020):11301-11313.PMID:33177838
- Endocrine (2024).PMID:38191984
- Mol Cells 46.4 (2023):231-244.PMID:36625319
- J. Cell. Mol. Med. 29.8 (2025):e70476.PMID:40275529
- Biomed Mater 18.6 (2023):065026.PMID:37852221
- Aging (Albany NY) 16.14 (2024):11339.PMID:39029955
- Front Toxicol 7 (2025):1598185.PMID:40376450
- Bmc Cancer 24.1 (2024):1-16.PMID:38475728
- Sci Rep-Uk 15.1 (2025):13282.PMID:40247060
- Cytokine 176 (2024):156507.PMID:38244240
- J Steroid Biochem (2023):106429.PMID:38035949
- J. Funct. Foods 128 (2025):106822.PMID:39486993
- Gene (2023):148050.PMID:38042211
- Microbiol Spectr (2024):e04142-23.PMID:38421191
- Cancer Biol Ther 25.1 (2024):2325126.PMID:38445610
- Sci Rep-Uk 15.1 (2025):11639.PMID:40185911
- Front Pharmacol 15 (2024):1397761.PMID:39104391
- Toxicol Appl Pharm (2023):116794.PMID:38142782
- Front. Oncol. 15 (2025):1536473.PMID:40303992
- Food Chem. Toxicol. (2025):115488.PMID:40288513
- Cell Death Dis 16.1 (2025):287.PMID:40223081
- Oral Dis (2024).PMID:38168877
- Gerontology (2024).PMID:38168028
- J Gen Virol 105.1 (2024):001944.PMID:38180473
- Bba-Mol Basis Dis (2024):167467.PMID:39159699
- Probiotics Antimicro (2025):1-16.PMID:40366615
- Endocrine (2024):1-14.PMID:38378893
- Biol 14.6 (2025):594.
- Front Pharmacol 15 (2024):1424624.PMID:25956975
- Spectrochim Acta A 237 (2020):118387.PMID:32416513
- Oncotargets Ther (2020):2511-2522.PMID:32280236
- Poultry Sci (2025):105339.
- Oncol Rep 53.2 (2025):1-9.PMID:39670302
- Bba-Mol Basis Dis (2024):167354.PMID:39004378
- Rsc Adv 13.32 (2023):22367-22374.PMID:37497090
- Gene (2024):148399.PMID:38518902
- Mol Cell Biochem (2024):1-15.PMID:39179754
- Epigenetics-Us 18.1 (2023):2254976.PMID:37691391
- Eur J Pharm Biopharm (2024):114417.PMID:39013493
- Cell Signal (2025):111815.PMID:40258578
- Front Pharmacol 16 (2025):1562839.PMID:40183104
- Frontiers in Pharmacology 16 (2025):1547685.
- Rsc Adv 13.33 (2023):23010-23020.PMID:37529353
- Langmuir 39.39 (2023):14162-14172.PMID:37722015
- J Biochem Mol Toxic 38.4 (2024):e23675.PMID:38488158
- Sci. Rep. 15.1 (2025):15556.PMID:40319036
- Sci Rep-Uk 14.1 (2024):19292.PMID:39164313
- Sci Rep-Uk 14.1 (2024):18929.PMID:39147857
- Sci Rep-Uk 10.1 (2020):20623.PMID:33244088
- Front Pharmacol 16 (2025):1517221.
- Acta Derm-Venereol 104 (2024).PMID:38590175
- Toxicol Appl Pharm 410 (2021):115361.PMID:33285147
- Transl Oncol 14.12 (2021):101219.PMID:34555726
- Sci Rep-Uk 14.1 (2024):25636.PMID:39465338
- Mol Immunol 153 (2023):126-134.PMID:36495817
- Bmc Med Genomics 15.1 (2022):1-19.PMID:36503492
- Am J Transl Res 13.10 (2021):11255.PMID:34786056
- J Cell Mol Med 28.20 (2024):e70163.PMID:39462268
- Bmc Cancer 21.1 (2021):1181.PMID:34740322
- Eur J Pharmacol (2025):177497.PMID:40058753
- Zool Res 45.5 (2024):1001.PMID:39147715
- Faseb J 39.2 (2025):e70314.PMID:39825721
- Oncol Rep 46.6 (2021):1-12.PMID:34643248
- Front Oncol 14 (2024):1440906.PMID:39346724
- J Endocrinol Invest (2025):1-16.PMID:39786708
- Inflammation 45.1 (2022):372-386.PMID:34792688
- Eur J Pharmacol 983 (2024):176995.PMID:39277096
- J Biol Chem (2024):107499.PMID:38944125
- Sci Rep-Uk 15.1 (2025):794.
- Biotechnol Genet Eng (2023):1-17.PMID:36946780
- Mol Cell Biochem (2023):1-11.PMID:36939994
- Front. Pharmacol. 16 (2025):1545498.
- Biotechnol Genet Eng (2023):1-15PMID:37018431
- Brain Res Bull (2025):111245.PMID:39924054
- Environ Toxicol (2023).PMID:37052456
- Zool Res 45.5 (2024).PMID:39147715
- Bmc Cancer 21.1 (2021):1-15.PMID:34130645
- Pediatr Res (2024):1-10.PMID:38658662
- Metab Brain Dis 38.1 (2023):255-267.PMID:36445630
- J Inflamm Res (2024):8009-8026.PMID:39507268
- Bioscience Rep 43.5 (2023):BSR20230028.PMID:37083601
- Brain Res Bull 187 (2022):1-10.PMID:35738501
- J Inflamm Res (2024):10533-10546.PMID:39659750
- J Immunother (2024):10-1097.PMID:38847148
- Front Pharmacol 15 (2024):1453938.PMID:39654626
- Bmc Cancer 22.1 (2022):1-14.PMID:35387643
- Front Pharmacol 16 (2025):1536091.PMID:40041493
- Oncol Rep 53.2 (2025):1-9.PMID:39670302
- J Oncol 2022 (2022).PMID:35518787
- Sci Rep-Uk 14.1 (2024):14438.PMID:38910141
- Int J Mol Med 49.3 (2022):1-11.PMID:35014672
- Brain Res Bull (2024):111165.PMID:39667505
- Hum Cell 35.4 (2022):1140-1158.PMID:35581525
- Thorac Cancer 13.5 (2022):761-770.PMID:35098684
- Neurotox Res 40.6 (2022):1895-1901.PMID:36223054
- Metab Brain Dis 37.7 (2022):2349-2362.PMID:35779150
- Front Oncol 14 (2024):1471109.PMID:39582546
- J Cancer 11.5 (2020):1162.PMID:31956362
- Pharm Biol 60.1 (2022):1436-1448.PMID:35938494
- Mol Cell Biochem (2025):1-20.PMID:40032749
- Hypertens Res 45.4 (2022):650-664.PMID:35082376
- Sci Rep-Uk 14.1 (2024):27063.PMID:39511266
- J Biochem Mol Toxice 36.11 (2022):e23200.PMID:35997646
- J Inflamm Res (2024):8009-8026.PMID:39507268
- Cancer Biol Ther 25.1 (2024):2355705.PMID:38778753
- Mol Immunol 152 (2022):27-34.PMID:36244215
- Bmc Complement Med 24 (2024).PMID:38783288
- J. Biomed. Mater. Res. Part A 113.3 (2025):e37893.PMID:40059713
- J Pharmacol Exp Ther 388.3 (2024):827-845.PMID:38262745
- Bioorgan Med Chem 72 (2022):116994.PMID:36087428
- Front Genet 13 (2022):932973.PMID:36118847
- Heliyon (2024).PMID:38707472
- Neuroscience (2023).PMID:37286157
- Oncol Rep 46.1 (2021):1-12.PMID:34080652
- Molecules 30.3 (2025):549.PMID:39942654
- Bioorgan Med Chem 82 (2023):117234.PMID:36906964
- Bmc Cancer 24.1 (2024):465.PMID:38622522
- Arch Biochem Biophys (2025):110352.PMID:39978618
- Eur J Pharmacol (2025):177343.PMID:39900330
- Acta Diabetol 58.9 (2021):1251-1267.PMID:33907874
- Arthritis Res Ther 27.1 (2025):12.PMID:39838477
- J Ethnopharmacol 278 (2021):114275.PMID:34087404
- Toxicol Sci (2024):kfae054.PMID:38636494
- Metabolomics 20.5 (2024):112.PMID:39369160
- Ann Transl Med 10.24 (2022).PMID:36660714
- Front Genet 13 (2022):959109.PMID:36147489
- Neurochem Res (2023):PMID:37127800
- Environ Toxicol (2024).PMID:39400944
- Toxicol Appl Pharm 422 (2021):115559.PMID:33961903
- Sci Rep-Uk 14.1 (2024):21680.PMID:39289451
- Bmc Chem 18.1 (2024):1-11.PMID:39396055
- Rsc Adv 14.18 (2024):12864-12872.PMID:38650686
- S Afr J Bot 177 (2025):445-456.
- CURR ISSUES MOL BIOL 47.5 (2025):314.
- Bba-Gen Subjects (2024):130628.PMID:38642815
- Heliyon (2024):.PMID:37461802
- J Orthop Surg Res 18.1 (2023):910.PMID:38031136
- Acta Bioch Bioph Sin 56.10 (2024):1473.PMID:38826133
- Pathogens 14.2 (2025):182.
- Bioengineered 13.1 (2022):1518-1529.PMID:34986734
- Mol Cell Biochem 473 (2020):179-192.PMID:32627113
- Planta Med 89.05 (2023):493-507.PMID:35716667
- Mol Med Rep 31.3 (2025):1-10.
- Dis Markers 2022 (2022).PMID:35845132
- Bioengineered 13.1 (2022):455-468.PMID:34967265
- J Orthop Surg Res 19.1 (2024):709.PMID:39487541
- Future Microbiol 0 (2024).PMID:38629914
- J Mol Neurosci 72.2 (2022):433-440.PMID:35023001
- Toxicol Appl Pharm (2025):117232.PMID:39832565
- N-S Arch Pharmacol (2024):1-21.PMID:39549064
- Shock 59.3 (2023):426-433.PMID:36609531
- Dis Markers 2022 (2022).PMID:35845132
- Technol Cancer Res T 19 (2020).PMID:33111613
- Chem Biol Drug Des 104.1 (2024):e14580.PMID:39031936
- Tissue Cell (2024):102477.PMID:39018712
- Mol Cell Biochem 476 (2021):1387-1400.PMID:33389498
- Virus Res 313 (2022):198748.PMID:35304133
- Bioengineered 13.3 (2022):6807-6818.PMID:35246020
- Bioengineered 13.3 (2022):6650-6664.PMID:35230214
- Bioengineered 13.4 (2022):10335-10349.PMID:35435110
- Curr Pharm Biotechno 23.8 (2022):1101-1108.PMID:34463226
- Cell Transplant 33 (2024).PMID:38334047
- Mol Carcinogen (2025).PMID:40222040
- Chem Biol Drug Des 99.6 (2022):828-838.PMID:35184389
- Front Med-Lausanne 11 (2025):1534120.PMID:39882536
- Cell Mol Biol Lett 24.1 (2019):1-14.PMID:31889958
- J Mech Behav Biomed (2025):106963.
- Luminescence 39.1 (2024):e4671.PMID:38286599
- Chem Biol Drug Des 105.4 (2025):e70111.PMID:40247760
- Discov Oncol 15.1 (2024):309.PMID:39060620
- Clinics 77 (2022).PMID:35662010
- Bioengineered 13.5 (2022):11987-12002.PMID:35603556
- Oncol Res 32.6 (2024):1129.PMID:38827325
- Mol Biotechnol 64.12 (2022):1367-1375.PMID:35639278
- J Mol Neurosci 74.1 (2024):30.PMID:38478195
- Shock (2023):10-1097.PMID:37179246
- WORLD J GASTRO ONCOL 17.3 (2025):102187.PMID:40092949
- Bmc Cancer 24.1 (2024):1-21.PMID:39085784
- Bmc Oral Health 24 (2024):1421.PMID:39574093
- METABOLITES 15.5 (2025):290.
- Microb Pathogenesis (2024):107055.PMID:39442820
- Acta Bioch Bioph Sin 56.10 (2024):1473.PMID:38826133
- Mol Biol Rep 52.1 (2025):212.PMID:39921807
- Mol Med Rep 28.5 (2023):1-12.PMID:37732544
- Discov Oncol 15.1 (2024):280.PMID:39004679
- Pharmacology 108.2 (2023):166-175.PMID:36724745
- Int J Chronic Obstr (2023):919-932.PMID:37229441
- J Tradit Chin Med 43.4 (2023):744.PMID:37454259
- Toxicon (2025):108293.PMID:39999927
- Kaohsiung J Med Sci (2025):e70031.PMID:40387410
- Eur Rev Med Pharmaco 23.5 (2019).PMID:30915737
- Bioengineered 12.1 (2021):5985-5995.PMID:34477479
- Bioengineered 12.1 (2021):6687-6702.PMID:34516362
- Microb Pathogenesis (2025):107720.PMID:40403987
- Bosn J Basic Med Sci 20.3 (2020):357.PMID:31733641
- CURR DRUG DELIV (2025).PMID:39976030
- Bioengineered 12.1 (2021):8698-8708.PMID:34697986
- MED ONCOL 42.4 (2025):1-13.PMID:40072654
- Tissue Cell (2025): 102903.
- Biomarkers (2024):1-12.PMID:38568742
- Luminescence 40.2 (2025):e70116.PMID:39914999
- N S Arch Pharmacol (2023):1-13.PMID:37160483
- Eur J Med Res 30.1 (2025):54.PMID:39865289
- Toxicol Lett (2025).PMID:40412772
- Mol Brain 18.1 (2025):1-23.PMID:39962509
- Luminescence 40.2 (2025):e70095.PMID:39924164
- J Pharm Pharmacol 71.9 (2019):1412-1420.PMID:31282010
- Gene 932 (2025):148898.PMID:39209182
- Hum Mol Genet (2024):ddae122.PMID:39216871
- J Sci Food Agr (2024).PMID:39394871
- Pathogens 14.2 (2025):182.
- J ORTHOP SURG RES 20.1 (2025):443.PMID:40319252
- Genomics (2025):111009.PMID:39864635
- FEBS Open Bio 12.1 (2022):146-153.PMID:34644456
- Plos One 19.12 (2024):e0316362.PMID:39724098
- J Orthop Surg Res 19.1 (2024):709.PMID:39487541
- Acta Bioch Bioph Sin 56.5 (2024):776.PMID:38495003
- ONCOL LETT 29.6 (2025):1-12
- Bioengineered 12.2 (2021):11451-11460.PMID:34895055
- Mol Biotechnol (2024):1-15.PMID:38771419
- Bioengineered 12.2 (2021):11936-11946.PMID:34784841
- Dis Markers 2022 (2022).PMID:35968506
- Mol. Med. Rep. 31.5 (2025):1-12.PMID:40145553
- Toxicol Appl Pharm (2025):117264.PMID:39952301
- Biochem Bioph Res Co (2025):151972.
- Shock (2023):10-1097.PMID:37988072
- J Mol Histol (2024):1-8.PMID:38758519
- Curr Stem Cell Res T 19.7 (2024):1021-1028.PMID:37861049
- Cell Stress Chaperon 25 (2020):955-968.PMID:32572784
- Int J Chronic Obstr (2025):623-639.
- Clin Exp Pharmacol P 50.11 (2023):903-913.PMID:37635387
- Tissue Cell 80 (2023):102001.PMID:36565506
- Mol Biol Rep 51.1 (2024):1-13.PMID:39422836
- J Appl Toxicol 42.5 (2022):830-840.PMID:34708435
- Thorac Cancer 14.9 (2023):815-826.PMID:36737402
- AESTHET PLAST SURG (2025):1-15.PMID:40180642
- J Membrane Biol 253 (2020):43-55.PMID:31820013
- Cell Mol Biol 70.2 (2024):161-168.PMID:38430026
- Curr Cancer Drug Tar 24.9 (2024):975-986.PMID:38204265
- J Int Med Res 48.4 (2020).PMID:31891283
- Pediatr Surg Int 40.1 (2024):63.PMID:38431920
- TRANSL ANDROL UROL 14.2 (2025):36078-36378.PMID:40114844
- Immunobiology (2024):152832.PMID:38943814
- PeerJ 13 (2025):e19083.
- Chin J Integr Med (2024):1-11.PMID:38941045
- Cell Mol Biol 69.12 (2023):223-231.PMID:38063090
- N S Arch Pharmacol (2023):1-13.PMID:37052642
- Pathol Res Pract 217 (2021):153284.PMID:33249400
- Cell J 25.8 (2023):554.PMID:37641417
- Cancer Biomark 42.5 (2025):18758592241306263.
- Am J Transl Res 15.7 (2023):4873.PMID:37560235
- Oncol Res 33.1 (2024):103.PMID:39735676
- Particul Sci Technol (2025):1-13.
- J Cardiothorac Surg 18.1 (2023):222.PMID:37420297
- Anticancer Res 43.8 (2023):3411-3418.PMID:37500147
- Vet Parasitol 331 (2024):110284.PMID:39126893
- Pediatr Surg Int 39.1 (2022):54.PMID:36534156
- J Asian Nat Prod Res 24.11 (2022):1078-1085.PMID:34935551
- Exp Ther Med 20.4 (2020):3290-3300.PMID:32855700
- World J Oncol (2024):.
- J Int Med Res 48.4 (2020).PMID:31891283
- J Toxicol Sci 48.8 (2023):469-479.PMID:37532580
- Chem Biodivers 18.4 (2021):e2001007.PMID:33624427
- J Int Med Res 49.3 (2021).PMID:33730930
- Bmc Pediatr 25.1 (2025):42.PMID:39825267
- Adv Clin Exp Med 30.10 (2021):1031-1041.PMID:34329545
- J Obstet Gynaecol Re PMID:39169658
- Anim Biotechnol (2023):1-12.PMID:36756956
- Clin Hemorheol Micro (2023):1-15.PMID:37212088
- Chem Biodivers 18.4 (2021):e2001007.PMID:33624427
- Genes Genom (2023):1-15.PMID:37318704
- Hematology 28.1 (2023):2220522.PMID:37272581
- Fitoterapia (2024):106245.PMID:39393711
- Biochem Genet (2023):1-18.PMID:37148331
- Am J Transl Res 14.10 (2022):6964.PMID:36398260
- Curr Mol Pharmacol 17.1 (2024).PMID:36946474
- Balk Med J 41.6 (2024).PMID:39319820
- J Obstet Gynaecol Re PMID:39169658
- Neuroreport 33.10 (2022):413.PMID:35623086
- Clin Cosmet Inv Dent (2022):87-94.PMID:35411190
- Endocr Metab Immune 24.1 (2024):153-162.PMID:37150983
- Aesthet Plast Surg (2023):1-11.PMID:38148357
- Cell J 24.1 (2022):15.PMID:35182060
- Int J Gen Med (2022):3199-3213.PMID:35342302
- Cell J 24.1 (2022):15.PMID:35182060
- Balk Med J 41.6 (2024):491.PMID:39319820
- Open Life Sci. 20.1 (2025):20221056.
- Comb Chem High T Scr (2024).PMID:35894460
- Chin J Integr Med (2025):1-10.PMID:40338446
- Clin Cosmet Inv Dent (2022):87-94.PMID:35411190
- Mol Cell Probe (2025):102031.
- Ann Hepatol 27.4 (2022):100700.PMID:35338010
- Discov Oncol 14.1 (2023):1-10.PMID:37907650
- Vet Parasitol (2024):110284.PMID:39126893
- Bmc Nephrol 24.1 (2023):314.PMID:37884904
- Acta Histochem 125.8 (2023):152097.PMID:37813066
- Vet Microbiol (2025):110495.
- Int J Gen Med (2022):3199-3213.PMID:35342302
- Iran J Immunol (2023).PMID:37865874
- Transl Cancer Res 13.4 (2024).PMID:38737693
- J Cardiothorac Surg 20.1 (2025):53.PMID:39794868
- J Herbs Spices Med P (2025):1-16.
- J Craniofac Surg 33.4 (2022):1250-1254.PMID:36041089
- Crit Rev Eukar Gene (2025).
- Transpl P 52.1 (2020):398-405.PMID:31928781
- Bioact Mater 44 (2025):220-235.
- Drug Resist Update (2024):101161.
- Carbohyd Polym (2024):122897.
- Chin Herb Med (2024).
- Biosens Bioelectron (2024):116875.
- Int J Mol Sci 25.20 (2024):11184.
- Acs Omega (2024).
- Drug Des Dev Ther (2024):5841-5851.
- J Funct Foods 123 (2024):106621.
- Antioxidants 13.11 (2024) 1320.
- Antioxidants-Basel 13.11 (2024):1289.
- bioRxiv (2024) 2024-10.
- Ecotox Environ Safe 286 (2024):117233.
- Gene (2024) 149056.
- Front Pharmacol 15:1453938.
- New J Chem 48.11 (2024):5068-5073.
- Biomedicines 12.11 (2024):2543.
- Heliyon (2024).
- Faseb J 38.15 (2024):e23876.
- Heliyon (2024).
- Toxins 16.7 (2024):319.
- Am J Cancer Res (2024):2852-2867.
- Netw Model Anal Hlth 13.1 (2024):1-15.
- hLife (2024).
- J Transl Med 22.1 (2024) 1-16.
- Biomed Pharmacother 179 (2024):117370.
- Bioorg Chem (2024):107731.
- Phytother Res 34.4 (2020):836-845.
- Acs Appl Mater Inter (2024).PMID:39167710
- Small (2024):2402308.PMID:3911
- Viruses 16.8 (2024):1239.
- Int J Mol Sci 25.15 (2024):8340.
- Front Bioeng Biotech 12 (2024):1460870.
- J Tradit Compl Med (2024).
- Indian J Pharm Sci 86.3 (2024).
- Heliyon (2024).
- Acs Omega (2024).
- Nutrients 16.14 (2024):2346.
- Nutrients 16.14 (2024):2314.
- Rev Bras Farmacogn (2024):1-7.
- Acs Appl Nano Mater (2024).
- J Hazard Mater (2024) 135735.
- scienceasia (2024).
- Phytomedicine (2024):156091.
- J Hazard Mater (2024):136113.
- Chem Eng J (2024):156542.
- Pharmaceuticals-Base 17.10 (2024):1295.
- Int J Mol Sci 25.19 (2024):10496.
- Int J Mol Sci 25.19 (2024):10339.
- Adv Funct Mater 29.50 (2019):1906690.
- bioRxiv (2025):2025-01.
- J Transl Med 22.1 (2024):1-16.
- Front Pharmacol 15 (2024):1384141@0.jpg
- J Pharm Anal (2024):101053.
- Bioact Mater 36 (2024):524-540.
- Green Process Synth 13.1 (2024):20240050.
- J Nutr Biochem (2024):109769.PMID:9276944
- Int J Bioprinting (2024).
- Int J Biol Sci 20.12 (2024):4713-4730.
- Bioact Mater 43 (2025):32-47.
- J Integr Med-Jim (2024).
- Talanta (2024):126381.
- Front Oncol 12 (2022):851300.PMID:35392243
- Mol Cell Biochem (2024) 1-13.
- Int J Mol Sci 22.16 (2021):8898.PMID:34445601
- Appl Entomol Zool (2022):1-14.
- J Inflamm Res 14 (2021):3523.PMID:34335041
- Fiber Polym (2022):1-9.
- Biomed Res Int 2021 (2021).PMID:34373837
- Dis Markers 2021 (2021):1-12.PMID:34413913
- Nanomaterials 11.8 (2021):2008.PMID:34443839
- View-China 4.2 (2023):20220052.
- J Inflamm Res (2023):1045-1057.PMID:36936349
- Int J Mol Sci 24.7 (2023):6279.PMID:37047251
- Cells 10.9 (2021):2399.PMID:34572049
- Oxid Med Cell Longev 2021 (2021):1-30.PMID:34257808
- Nano Res (2022):1-8.
- Phytochem Lett 46 (2021):79-89.
- Int J Pept Res Ther 29.2 (2023):19.
- J Environ Pathol Tox 42 (2023).PMID:36749091
- Oxid Med Cell Longev 2023 (2023).PMID:36778205
- Mol Med 29.1 (2023):6.PMID:36647009
- Poultry Sci 102.4 (2023):102511.PMID:36805396
- Sensor Actuat B-Chem 381 (2023):133437.
- Carbohyd Polym 277 (2022):118869.PMID:34893274
- Drug Develop Res 84.2 (2023):312-325.PMID:36658741
- bioRxiv (2023):2023-05.PMID:37292976
- J Clean Prod 315 (2021):128201.
- Exp Ther Med 25.2 (2023):1-10.PMID:PMID_36684652
- Diabet Metab Synd Ob (2021):2255-2268.PMID:34045875
- Biomaterials 272 (2021):120757.PMID:33798960
- Front Cell Neurosci 15 (2021):569424.PMID:33815059
- Open Med-Warsaw 16.1 (2021):558-569.PMID:33869778
- bioRxiv (2022):2022-04.
- Phytomedicine 86 (2021):153565.PMID:33945919
- Acs Appl Polym Mater 4.10 (2022):7014-7024.
- Mol Biotechnol 63.8 (2021):686-693.PMID:33977498
- Compos Part B-Eng 220 (2021):108984.PMID:35988999
- Cancer Res Commun 2.6 (2022):471-482.PMID:36338546
- Acs Appl Bio Mater 4.6 (2021):5026-5032.
- Curr Issues Mol Biol 45.5 (2023):4017-4034.PMID:37232725
- Exp Eye Res 209 (2021):108642.PMID:34058232
- J Inflamm Res 14 (2021):2445.
- HemaSphere 5.7 (2021).PMID:34136753
- Adv Funct Mater (2023):2214998.
- Oncol Lett 26.1 (2023):1-17.
- Colloid Surface B 228 (2023):113364.PMID:37290201
- Cancer Manag Res (2021):4709-4723.PMID:34163246
- Ceram Int 49.15 (2023):25953-25963.
- Food Hydrocolloid 120 (2021):106980.
- J Thorac Dis 15.4 (2023):2198.PMID:37197522
- Aquaculture 564 (2023):739060.
- Int J Mol Sci 24.2 (2023):1243.PMID:36674761
- J Biol Chem 297.2 (2021).PMID:33722608
- Phytomedicine 103 (2022):154199.PMID:35679793
- Phytomedicine 105 (2022):154330.PMID:35905566
- Carbohyd Polym 295 (2022):119878.PMID:35989018
- Transl Cancer Res 11.1 (2022):206.PMID:35261897
- Transl Cancer Res 11.6 (2022):1781.PMID:35836521
- Tissue Cell 76 (2022).PMID:35182988
- Mater Today Adv 15 (2022):100271.
- Pharmacol Res 177 (2022):106112.PMID:35122955
- Oxid Med Cell Longev 2022 (2022).PMID:35770048
- Foods 11.11 (2022):1575.PMID:35681325
- Cytotechnology 74.3 (2022):421-432.PMID:35733701
- Oxid Med Cell Longev 2022 (2022).PMID:35936222
- Acta Trop 232 (2022):106519.PMID:35584779
- Clin Transl Disc 2.2 (2022):e49.
- Nano Today 43 (2022):101416.
- Front Cell Infect Mi 12 (2022):423.PMID:35573770
- Front Pharmacol 13 (2022):899775.PMID:35571096
- Int J Mol Sci 23.8 (2022):4128.PMID:35456945
- Bba-Mol Cell Biol L 1867.6 (2022):159137.PMID:35247568
- Bioengineered 13.4 (2022):9145-9155.PMID:35363601
- J Cancer 13.6 (2022):1808.PMID:35399733
- Oncol Lett 23.5 (2022):1-8.PMID:35350588
- J Oncol 2022 (2022).PMID:35909906
- Dis Markers 2022 (2022).
- Mater Today Chem 27 (2023):101331.
- Biomaterials Advances 142 (2022):213165.PMID:36341744
- Acs Appl Bio Mater 6.1 (2023):74-82.PMID:36603189
- PeerJ 9 (2021):e11482.PMID:34820154
- Tissue Cell 74 (2022):101680.PMID:34808429
- Histol Histopathol 35.9 (2020):919-927.PMID:32282924
- Mol Cell Toxicol (2022):1-11.
- Cells 11.22 (2022):3580.PMID:36429010
- J Agr Food Chem 70.44 (2022):14220-14234.PMID:36300841
- Cancers 14.21 (2022):5218.PMID:36358640
- Nano Today 41 (2021):101326.
- Pharmaceutics 14.10 (2022):2157.PMID:36297592
- Discov Oncol 13.1 (2022):71.PMID:35941331
- Exp Ther Med 23.2 (2022):1-9.PMID:35069838
- Phytother Res 37.2 (2023):477-489.PMID:36199227
- Cancers 14.19 (2022):4645.PMID:36230568
- Molecules 27.16 (2022):5337.PMID:36014573
- Exp Ther Med 23.2 (2022):1-9.PMID:35069839
- Bio-Des Manuf 5.2 (2022):358-370.
- J Oncol 2022 (2022).PMID:36059813
- Viruses 14.8 (2022):1783.PMID:36016405
- Phytochem Lett 47 (2022):174-179.
- Int J Mol Sci 23.16 (2022):9014.PMID:36012290
- Front Cell Dev Biol 8 (2021):568304.PMID:33763412
- Front Pharmacol 11 (2021):618262.PMID:33551818
- J Cancer 15.13 (2024):4244-4258.
- New J Chem (2024).
- New J Chem 48.11 (2024):5068-5073.PMID:PMID
- Exp Ther Med 27.5 (2024):1-16.
- Drug Dev Res 81.4 (2020):437-443.PMID:31904108
- Fitoterapia (2024):105884.
- Eur J Med Chem (2024):116323.PMID:(2024) 116323.
- Mater Design (2024):112812.
- J Funct Foods 114 (2024):106076.
- Front Immunol 15 (2024):1326137.
- Antioxidants Basel 13.3 (2024):264.
- Kaohsiung J Med Sci (2024).PMID:38340032
- J Agr Food Chem (2024).
- Mater Res Express 7.2 (2020):025401.
- J Ethnopharmacol (2024):117867.PMID: 38342155
- J Biomed Nanotechnol 20.1 (2024):133-142.
- Front Immunol 15 (2024):1347683.PMID:38343537
- bioRxiv (2024):2024-02.
- Mater Today Bio (2024):100958.
- Cell Death Dis 15.1 (2024):97.PMID:38286802
- Mol Ther Nucl Acids (2020).
- Int J Mol Sci 25.2 (2024):919.
- ACS Appl. Eng. Mater. (2025).
- Cell Biomaterials (2025):
- Nanoscale (2024).
- Phytomedicine (2024):155347.
- bioRxiv (2024):2024-05.
- Nat Commun 15.1 (2024):4969.PMID:38862489
- Heliyon (2024).
- Pharmaceutics 16.6 (2024):744.
- Phytomedicine (2024):155767.
- Molecular Therapy Oncology (2024).
- Theranostics 14.7 (2024):2687.
- Front Pharmacol 15 (2024):1389976.
- Available at SSRN 4830418.
- Phytomedicine (2024):155744.
- Adv Funct Mater (2024):2400489
- Mater Express 10.1 (2020):102-111.
- Transl Cancer Res 13.4 (2024).
- Phytomedicine (2024):155656.
- Pharmaceuticals-Base 17.4 (2024):515.
- Cells 13.9 (2024):728.
- Applied Sciences 14.8 (2024):3186.
- Adv Funct Mater (2024):2402663.
- Acs Pharmacol Transl (2024).
- Heliyon (2024).
- Acs Appl Opt Mater (2024).
- Redox Biol (2024):103143.
- Sensors & Diagnostics (2024).
- Adv Funct Mater (2024):2305827.
- Anal Cell Pathol 2020 (2020).PMID:33354489
- Front Oncol 10 (2020):387.
- Cell Mol Biol 69.5 (2023):44-50.PMID:37571902
- Biocell 47.8 (2023).
- Vaccines Basel 11.7 (2023):1190.PMID:37515006
- Poultry Sci 102.10 (2023):102946.PMID:37542939
- J-Ethnopharmacol 318 (2024):116969.PMID:37516391
- Int Immunopharmacol 123 (2023):110709.PMID:37515849
- Crystals 13.7 (2023):1067.
- Appl Entomol Zool 58.4 (2023):303-313.
- Aging (Albany NY) 15.12 (2023):5713PMID:37390121
- ACS omega 8.30 (2023):27146-27155.PMID:37546678
- Exp Ther Med 26.5 (2023):1-15.PMID:37869642
- J King Saud Univ Sci 32.7 (2020):2912-2919.
- Plos Pathog 19.4 (2023):e1011305.PMID:37053288
- Iscience 26.9 (2023).PMID:37670789
- Front Microbiol 14 (2023).PMID:37645224
- Acta Pharmacol Sin 44.1 (2023):255-257.PMID:35773339
- Mol Ther-Nucl Acids (2020).
- Nanophotonics 11.22 (2022):5089-5100.
- J Pharm Sci 75.10 (2020):505-509.PMID:33305726
- Int J Mol Sci 21.21 (2020):8095.PMID:33142980
- Front Mar Sci 9 (2022):861117.
- Chem Eng J 474 (2023):145358.
- Int J Nanomed (2023):5423-5440.PMID:37767196
- Adv Funct Mater (2023):2309690.
- Vaccines-Basel 11.11 (2023):1678.PMID:38006011
- Oncol Rep 51.2 (2024):1-13.PMID:38131250
- Biomed Pharmacother 170 (2024):116042.PMID:38118351
- Int J Gen Med (2023):5817-5839.PMID:38106976
- Int J Biol Sci 20.2 (2024):433-445.PMID:37379961
- Pharmaceutics (2023) 15(12):2713.
- Food Sci Nutr (2023).
- Biocell 47.8 (2023).
- Ann Hematol (2023):1-12.
- Crit Rev Eukar Gene 34 (2024).
- Front Immunol 14 (2023).PMID:37901236
- J-Ethnopharmacol 318 (2024):116961.PMID:37541404
- Int J Mol Sci 24.19 (2023):15003.PMID:37834451
- Biochip J (2023):1-10.
- Pharmaceuticals-Base 16.10 (2023):1482.PMID:37895953
- Mol Cell Toxicol (2023):1-10.
- Trop J Pharm Res 22.9 (2023):1857-1862.
- Trop J Pharm Res 22.9 (2023):1779-1784.
- Sensor Actuat B Chem 394 (2023):134396.
- Int J Mol Sci 24.21 (2023):15545.PMID:37958531
- Mol Cell Oncol 10.1 (2023):2238873.PMID:37649964
- Microorganisms 11.9 (2023):2258.PMID:37764102
- Int J Mol Sci 25.24 (2024):13439.
细胞计数试剂盒-8(CCK-8)使用WST-8(2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酚基)-2H-tetrazolium,单钠盐),在存在电子载体1-Methoxy PMS的情况下,产生水溶性福尔马定染料进行方便的测定。 CCK-8溶液直接加入到细胞中,无需预先混合组分。 WST-8被细胞脱氢酶生物还原为可溶于组织培养液的橙色福尔马定产物。 产生的福尔马定量与活着的细胞数量成正比。由于CCK-8溶液非常稳定且具有很小的细胞毒性,因此可以进行更长时间(例如24至48小时)的孵育。
细胞计数试剂盒 - 8允许灵敏度高、用于增殖和毒性测定中确定存活细胞数量的显色法检测。 检测灵敏度比MTT、XTT或MTS等任何其他四唑盐都要高。
图1:Cell Counting Kit-8(CCK-8)的工作机制。
细胞数量确定
1.将细胞悬浮液(100μL/孔)接种在96洞孔板中。将板在潮湿的培养箱中预孵育(例如,在37℃,5%CO2下)。
2.向板的每个孔中加入10μLCCK8溶液。注意不要在孔洞中引入气泡,因为它们会干扰O.D.读数。
3.将培养板在培养箱中孵育1-4小时。
4.使用酶标仪测量450 nm处的吸光度。
细胞增殖和细胞毒性测定
1.将种子细胞以103-104个细胞/孔的密度在96孔板中在100μL培养基中培养。将细胞在CO2培养箱中于37℃培养24小时。
2.将不同浓度的待测物质加入板中。
3.将培养板在培养箱中孵育适当的时间(例如,6,12,24或48小时)。
4.使用重复移液器向板的每个孔中加入10μLCCK8溶液。注意不要在孔洞中引入气泡,因为它们会干扰O.D.读数。
5.将培养板在培养箱中孵育1-4小时。
6.在读取孔板之前,重要的是在轨道振动器上轻轻混合1分钟,以确保颜色均匀分布。
7.使用酶标仪测量450 nm处的吸光度。
数据分析
统计分析有几种方法,您可以选择使用O.D.值或细胞数量,我们提供其中一种方法。
细胞存活率(%)= [(As-Ab)/(Ac-Ab)]×100
抑制率(%)= [(Ac-As)/(Ac-Ab)]×100
As =实验孔吸光度(含有细胞,培养基,CCK-8和待测化合物的孔的吸光度)。
Ab =空白孔吸光度(含有培养基和CCK-8的孔的吸光度)。
Ac =对照孔吸光度(含有细胞,培养基和CCK-8的孔的吸光度)。
制作标准曲线
1. 细胞计数板计数细胞悬液中的细胞数。
2. 使用培养基,等比稀释细胞悬液为一个浓度梯度,通常需要5-7个浓度梯度,每组几个复孔。然后接种细胞。(注意每孔的细胞数量。如果您将细胞悬液稀释在管中,在加入培养板的孔之前,请小心再次混匀细胞。每孔中细胞悬液的体积应该是一致的。)
3. 培养直至细胞贴壁(通常2-4小时),然后每100 μl培养基加入10 μl CCK-8。继续孵育1-4小时,用酶标仪测量450nm处的吸光度。制作出一条以细胞数为X轴坐标,O.D.值为Y轴坐标的标准曲线。
可以基于该曲线确定待测样品的细胞数。使用此标准曲线的先决条件是培养检测条件相同。
注意事项
1. 确保药物和CCK8均匀分布在培养基中。
2. 细胞增殖越多, 颜色越深; 细胞毒性越强,颜色越浅。
3. 对于贴壁细胞,每孔至少需要1000个细胞(100 μl培养基)。对于白细胞,由于灵敏度较低,每孔至少需要2500个细胞(100 μl培养基)。推荐的96孔板每孔最大细胞数为25000。如果使用24孔或6孔板进行该检测,请计算相应的每孔的细胞数,并调整CCK-8的体积,使其为每孔总液体体积的10%。
4. 因为CCK8测定是基于活细胞中的脱氢酶活性,影响脱氢酶活性的条件或化学物质可能导致实际活细胞数与使用CCK-8测定活细胞数之间有差异。
5. WST-8可能与还原剂反应生成WST-8 formazan。如果使用还原剂 (例如一些抗氧化剂)会干扰检测。如果待检测体系中存在较多的还原剂,需设法去除。
6. 孵育2小时后,背景O.D.值一般为0.1-0.2单位。
7. 注意不要在孔中引入气泡,因为它们会干扰O.D.值。
8. 如果您想对CCK8溶液进行灭菌,请使用0.2 μm的膜过滤溶液。
9. 孵育时间因孔中细胞的类型和数量而异。通常,白细胞着色较弱,因此可能需要较长的孵育时间(长达4小时)或大量细胞(~105细胞/孔)。
10. 如果细胞悬液中存在高浊度, 测量并减去样品在600nm或更高波长的O.D值。
11. CCK8不能用于细胞染色。
12. 培养基中的酚红不会影响实验结果,酚红的吸光度可以在计算时,通过扣除空白孔中本底的吸光度而消去,因此不会对检测造成影响。
13. CCK8的毒性非常低,在CCK8测定完成后,相同的细胞可用于其他细胞增殖测定,例如结晶紫测定,中性红测定或DNA荧光测定。(除非细胞极为稀少,不推荐。)
14. 该试剂盒可用于大肠杆菌,但不能用于酵母细胞。
15. 在读取平板之前,您可以在摇床上轻柔混匀。
16. 我们建议将细胞接种在靠近培养板中央的孔中,最外围一圈孔中的培养基容易蒸发,可以用PBS,水或培养基填充这些孔。
17. 如果您没有450nm滤光片。您也可以使用吸光度在430和490nm之间的滤光片, 450nm滤光片具有最佳灵敏度。
18. 测量450nm处的吸光度,如果您需要进行双波长测定,作为参考波长可以测定650nm处的吸光度。
19. 药物中金属离子的存在可能会影响CCK8的灵敏度。终浓度为1mM的氯化亚铅、氯化铁、硫酸铜会抑制 5%、15%、 90% 的显色反应,使灵敏度降低。如果终浓度是10mM的话,将会100%抑制。
常见问题
Q:如何做空白对照?
A:可以用加相应量细胞培养基和CCK-8但不加细胞的孔作为空白对照,若担心所使用的药物会干扰检测,需设置加相应量细胞培养液、药物和CCK-8但不加细胞的孔作为空白对照。
Q:只加CCK-8不加细胞的空白孔,数值上增?
A:在整个实验过程中,随着时间的增加,如果细胞培养时间较长,请注意蒸发问题。因为反应环境温度较高,会有部分液体挥发。空白组虽然没有细胞,但是随着液体的挥发,药物浓度相当于提高了,所以空白对照的数值也会有轻微的变化(但不会有明显的变化)。如果很在意空白对照的数值,可将96孔板外围一圈加培养基、水或PBS 保湿。同时,可以把96孔板置于培养箱内靠近水盘的位置以缓解蒸发。将96孔板外围两圈用PBS铺板,这样挥发先挥发周边的,可以对内部及空白对照的影响降至最低。
Q:一般多久测一次值?
A:预实验时可以在0.5、1、2和4小时后分别用酶标仪检测,然后选取吸光度范围比较适宜的一个时间点用于后续实验。
Q:加入CCK8后暂时不测OD值,可以如何处理暂时保存?能够保存多久?
A:如果需要暂时不测定OD值,可以向每孔中加入10ul 0.1M HCl溶液或者1% w/v的SDS溶液,避光保存在室温,24小时内吸光度不会发生变化。
Q:如何保存?
A:CCK-8溶液在避光、0-5℃的条件下可以保存1 年,若长期不用可在-20℃下避光保存2年。建议分装后保存于-20℃,使用时提前于4℃解冻,请避免反复冻融,否则增加背景值,干扰实验结果。
Q:是否需要更换培养基?
A:不需要,若药物影响比较小的情况可以不更换培养基,直接扣除培养基中加入药物后的空白吸收即可。
Q:待测物质有氧化性或还原性该做何处理?
A:本试剂盒的检测依赖于脱氢酶催化的反应,如果待测物质有氧化性或还原性,可在加CCK-8之前更换新鲜培养基,去掉药物的影响。
如果实验中有还原剂,请检查背景的OD值,即在不含细胞的培养基中加入药物,然后加入CCK-8试剂在一定时间内检测,和不加药物的培养基进行比较(只有CCK-8试剂),如果O.D值明显偏高,则说明有反应。
Q:如果培养基颜色或PH已变化,是否需要更换新的培养基?
A:加入CCK-8时,如果细胞培养时间较长,培养基颜色或pH值已变化,建议换用新鲜的培养基。
Q:适用细胞?
A:因为测线粒体酶,所以真核细胞,有线粒体的均可,例如单细胞,动物细胞。但有细胞壁的细胞,例如植物细胞,酵母细胞,底物进入细胞效率低,不推荐。
Q:线性不佳原因?
A:细胞不均;培养液蒸发;还原剂;气泡;孔板周围一圈不能用,除非只培养少于一天的时间;细胞数目很重要,太少信号会低。
Q:金属毒性?
A:可以检测金属毒性,最好做空白对照。
Q:适用于那些细胞?其他还有哪些细胞可用?生物被膜内的活菌 (流感嗜血杆菌和绿脓杆菌)?
A:所有哺乳动物的细胞,细菌一般没有检测CCK8的,可以染色法,或者流式,最常规的方法,有报道, 比浊法测OD600。
Q:是否可以替代Brud或EDU法检测细胞增殖?
A:虽然CCK8通过检测对生长期的细胞内的脱氢酶来反应细胞的活性,而胸腺嘧啶插入法通过核苷酸类似物的方式插入合成的DNA来检测细胞的活性,这几种方法是有一定关联性的,CCK8可替换胸腺嘧啶插入法来检测细胞增殖,并且检测更方便。
Q:空白孔数值偏高,有可能是培养基长菌吗?或者其他原因?
A:空白孔数值偏高一般认为染菌造成的,很少出现这种问题。
Q:CCK8能检测组织吗
A:不能检测组织。
Q:是否可以检测T细胞
A:可以,T细胞长的慢,需延长孵育时间。
Q:当两个细胞混合培养时(骨髓间充质细胞,另一个是悬浮T细胞),培养一段时间后,只测骨髓间充质干细胞的生长、增殖情况,请问用CCK-8能测吗?怎么做?
A:对于两种细胞的情况:
1)如果测定贴壁细胞的生长状况,那么可以把悬浮的T细胞转移掉,使用上述第二种方法测定。
2)如果T细胞对于间充质细胞的生长很关键,不能取走,那么测定的活性程度是总量,此时,需要对每一个孔的T细胞进行估算,如果每个培养条件下T细胞总量接近,或者T细胞含量很少,远远小于间充质细胞,也可以测定,使用对照孔可以去除T细胞的影响。
3)如果T细胞可以短时间内除去,可以找个灵敏度高的CCK-8,信号强,建议细胞量合适的情况下(每一个孔10000个),30分钟左右出读数结果,测 定后,可以将T细胞再加入培养孔。 这样做对细胞的生长影响不大。但是如果做动力学,那么要考虑的因素多一些。
Q:CCK-8是通过和细胞内的脱氢酶反应而反映着细胞数量,假如细胞已经死亡,而脱氢酶活性还有,那么如何计算细胞数量?
A:多数情况下,细胞死亡过程中,细胞内的成分会降解,脱氢酶会有部分释放,但是活性和活细胞比,比较低。举例说:100个细胞死亡20小时后,释放的脱氢酶活力,不足5个活细胞的脱氢酶活力,也就是说,这种情况带来的误差5%以内。而且,这种细胞死亡,对同种细胞来说,情况是一样的,一般都是在不同的孔间比较同种细胞,所以系统误差是一样的,即系统误差会彼此消减。
应用&特点 |
1) 细胞增殖测定 - GlpBio细胞计数试剂盒-8(CCK-8)是水溶性的,在培养中是稳定的,并且是无毒的。 2) 细胞活力测定 - 代谢活性和染料产生与改变的活力成比例地变化。 3) 细胞因子测定 - 测量细胞因子诱导的增殖。如果需要,可以在研究结束时回收细胞并扩增。 4) 细胞毒性测定 - 来自细胞毒性化学物质的细胞死亡对颜色发展没有影响,只有活细胞将试剂转化为比色指示剂。试剂本身具有可忽略的毒性,并且通常对细胞是安全的。 |
运输方式 | 蓝冰运输。 |
储存条件 | 储存在4°C避光,可稳定长达12个月。储存在-20°C避光,可稳定长达2年。 |
用途 | 仅供研究使用!不能用于人体。 |
CCK-8 | MTT | MTS | SRB |
Solubility | Water soluble | Indissolvable | Water soluble | Indissolvable |
Detection Wavelength | 450nm | 490nm | 450nm | 510nm |
Character | Liquid | Solid | Liquid | Liquid |
Usage | No need to prepare | Prepare the solutions | Use it right after it was ready | Prepared beforehand |
Need to redissolve or not | NO | Yes,by DMSO | No | Yes,by Tris-base solution |
Convenience | +++ | ++ | +++ | + |
Detection speed | +++ | + | ++ | + |
Repeatability | +++ | + | ++ | ++ |
Stability | ++ | + | + | +++ |
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