Cell Counting Kit-8 (CCK-8)
目录号 : GK10001细胞计数试剂盒-8(CCK-8)是一种即用型的单瓶溶液,可快速、可靠地测量各种化学物质对细胞存活率和毒性的敏感度。
Sample solution is provided at 25 µL, 10mM.
- Adv Mater (2023):2307193.PMID:37951210
- Adv Mater (2024):2401538.PMID:38738793
- Adv Mater (2025):2413968.PMID:40223457
- Nat Microbiol (2025):1-16.PMID:39825096
- Cell Host Microbe 32.12 (2024):2195-2211.PMID:39610253
- Brain Behav Immun 110 (2023):60-79.PMID:36754245
- Adv Sci 7.9 (2020):1902938.PMID:32382476
- Nat Commun 15.1 (2024):3771.PMID:38704377
- Adv. Fiber Mater. (2025):1-17.
- Matter (2025).
- Nat Commun 15.1 (2024):72.PMID:38167723
- Nat Commun 14.1 (2023):3877.PMID:37391428
- ACS nano 17.7 (2023):7017-7034.PMID:36971310
- ACS nano (2025).PMID:39846120
- Acs Nano (2024).PMID:39361472
- Autophagy 18.5 (2022):1049-1061.PMID:34517786
- Cancer Commun (2024).PMID:38512764
- Acta Pharm Sin B (2025).
- Adv Sci (2023):2301107.PMID:37097746
- Adv Sci (2024):2401845.PMID:38757623
- Adv-Sci 10.27 (2023):2302640.PMID:37485650
- Adv Sci 10.31 (2023):2301120.PMID:37688357
- Brain Behav Immun (2024).PMID:38565398
- Cancer Commun 43.5 (2023):582-612.PMID:37005481
- Autophagy just-accepted (2024).PMID:39007910
- Nat Commun 16.1 (2025):3605.PMID:40240371
- Nat. Commun. 16.1 (2025):2551.
- Adv Sci (2024):2402450.PMID:38952061
- Adv Sci (2025):2417318.PMID:40472230
- Sci Adv 10.10 (2024):eadg7380.PMID:38457499
- Adv Sci (2024):2404365.PMID:39159143
- Adv Sci (2025):2417374.PMID:40213972
- Microbiome 12.1 (2024):152.PMID:39152482
- Adv Sci (2025):2413936.PMID:40047320
- Small 21.3 (2025):2409121.PMID:39544127
- J Exp Clin Canc Res 41.1 (2022):293.PMID:36199122
- Emerg Microbes Infec 12.1 (2023):2204164.PMID:37060263
- J Adv Res (2023).PMID:36933884
- Water Res (2024):121379.PMID:38422694
- Biomaterials (2025):123335.PMID:40222258
- Small (2024):2407674.PMID:39363789
- J Med Virol 95.10 (2023):e29171.PMID:37830751
- Nano Today 61 (2025):102560.
- Gut Microbes 16.1 (2024):2347757.PMID:38773738
- Mol Ther (2025).PMID:39810420
- Mol. Ther. 33.3 (2025):1134.PMID:39810420
- Theranostics 14.16 (2024):6161.PMID:39431016
- Cell Death Differ 30.10 (2023):2293-2308.PMID:37608075
- Small Methods (2024):2400223.PMID:38602202
- Theranostics 12.7 (2022):3488.PMID:35547749
- J Hazard Mater (2024):135904.PMID:39303616
- Mater Horiz (2024).PMID:39189308
- Mater Horiz (2024).PMID:39189308
- Gut Microbes 17.1 (2025):2446376.PMID:39718561
- Redox Biol (2023):102983.PMID:38064762
- J Control Release 365 (2024):301-316.PMID:38007195
- Cell Discov 6.1 (2020):96.PMID:33349633
- J Exp Clin Canc Res 39 (2020):1-13.PMID:32028978
- J Control Release 359 (2023):12-25.PMID:37244298
- Acta Biomater 157 (2023):321-336.PMID:36481504
- Environ Sci Technol (2024).PMID:39364562
- Water Res (2024):122403.PMID:39278116
- J Control Release 373 (2024):749-765.PMID:39084465
- J Exp Clin Canc Res 44.1 (2025):1-19.PMID:40069844
- Environ Sci Technol 56.21 (2022):15096-15107.PMID:36099323
- Sci Total Environ 878 (2023):162942.PMID:36940749
- Redox Biol 62 (2023):102663.PMID:36924682
- J Exp Clin Canc Res 41 (2022):1-15.PMID:34980201
- J Adv Res (2025).PMID:40373960
- J Control Release 360 (2023):647-659.PMID:37406817
- J Exp Clin Canc Res 43.1 (2024):187.PMID:38965580
- J Adv Res (2025).
- J Control Release 379 (2025):89-104.PMID:39756690
- Cell Discov 6.1 (2020):96.PMID:33349633
- J Control Release 349 (2022):254-268.PMID:35803328
- EBioMedicine 99 (2024).PMID:38101299
- J Exp Clin Canc Res 43.1 (2024):91.PMID:38528516
- J Immunother Cancer 12.3 (2024):e008054.PMID:38429070
- J Exp Clin Canc Res 41.1 (2022):1-15.PMID:34980201
- Clin Transl Med 14.1 (2024):e1539.PMID:38224186
- J Exp Clin Canc Res 41 (2022):1-15.PMID:34980201
- J Nanobiotechnol 21.1 (2023):354.PMID:37775761
- J Nanobiotechnol 23.1 (2025):1-22.PMID:39871272
- ENGINEERING-PRC (2025).
- J Nanobiotechnol 20.1 (2022):1-20.PMID:36123708
- Adv Healthc Mater 12.9 (2023):2202785.PMID:36541060
- Adv Healthc Mater (2025):2500845.PMID:40400499
- Environ Health Persp 131.6 (2023):067004.PMID:37267060
- Rare Met. (2025):1-23.
- Adv Healthc Mater 13.8 (2024):2303215.PMID:38112062
- J Neuroinflamm 20.1 (2023):1-16.PMID:36944954
- Cancer Lett (2024):216765.PMID:38408604
- Sci Total Environ 897 (2023):165514.PMID:37451464
- View-China (2025):20240103.
- Adv Healthc Mater 11.8 (2022):2102395.PMID:34874119
- Acta Biomater (2024).PMID:38452962
- Pharmacol Res 187 (2023):106563.PMID:36410674
- Cardiovasc Res 121.2 (2025):324-338.PMID:39658102
- J Immunother Cancer 12.10 (2024):e009416.PMID:39455095
- J Nanobiotechnol 22.1 (2024):1-21.PMID:38448959
- J Nanobiotechnol 20.1 (2022):1-20.PMID:36123708
- J Immunother Cancer 13.6 (2025):e011717.PMID:40473272
- Adv Healthc Mater (2024):2402877.PMID:39434433
- Adv Healthc Mater (2024):2401061.PMID:38849128
- J Nanobiotechnol 22.1 (2024):1-15.PMID:38790037
- Acs Appl Mater Inter 15.32 (2023):38294-38308.PMID:37542453
- Food Chem (2024):139505.PMID:38703732
- Cell Death Dis 13.5 (2022):497.PMID:35614040
- Acs Appl Mater Inter (2024).PMID:38345297
- Brit J Cancer (2024):1-9.PMID:38594370
- J Colloid Interf Sci (2025):.PMID:39755016
- Environ Pollut 338 (2023):122581.PMID:37748638
- Cancer Lett 611 (2025):217418.PMID:39725146
- Cell Mol Life Sci 79.7 (2022):382.PMID:35751755
- Acta Biomater (2025).PMID:40024424
- Cell Mol Life Sci 80.3 (2023):61.PMID:36763212
- Liver Int 43.4 (2023):840-854.PMID:36737842
- CANCER LETT (2025):217685.
- Mater. Today Bio (2025):101703.
- Acta Biomater (2025).PMID:39952883
- Acta Biomater 113 (2020):393-406.PMID:32629189
- Environ Pollut 334 (2023):122148.PMID:37419204
- Cancer Lett (2024):217418.PMID:39725146
- Pharmacol Res 180 (2022):106225.PMID:35452801
- J Colloid Interf Sci (2025):137652.
- Mol Ther-Nucl Acids 31 (2023):703-716.PMID:36923951
- Pharmacol Res (2024):107460.PMID:39393436
- Front Immunol 14 (2023):1130308.PMID:37006248
- Cell Mol Biol Lett 29.1 (2024):103.PMID:38997648
- Biomark Res 11.1 (2023):3PMID:36611218
- Cell Death Dis 13.10 (2022):858.PMID:36209201
- Pharmacol Res 195 (2023):106877.PMID:37524154
- ACS Appl Mater Inter 14.13 (2022).PMID:35344348
- Acs Appl Mater Inter 15.11 (2023).PMID:36897565
- Cell Commun Signal 22.1 (2024):1-19.PMID:38454413
- Cell Commun Signal 22.1 (2024):1-23.PMID:39696352
- Cell Death Dis 12.8 (2021):779.PMID:34365463
- Free Radical Bio Med 188 (2022):404-418.PMID:35787451
- INT J BIOL SCI 21.7 (2025):3099-3121.
- Phytomedicine 118 (2023):154944.PMID:37393830
- Phytomedicine (2023):155270.PMID:38096717
- Int J Biol Macromol (2024):129172.PMID:38176496
- Biomater Sci-UK 10.17 (2022):5006-5017.PMID:35815806
- Mater Today Bio (2024):101019.PMID:38516170
- Cell Mol Life Sci 81.1 (2024):143.PMID:38493426
- Acs Sensors (2024).PMID:38958530
- Acs Omega (2025).
- Int. J. Biol. Markers (2025):143683.PMID:40318724
- Environ Res (2024):118221.PMID:38246300
- Int J Biol Macromol 220 (2022):371-384.PMID:35970372
- Free Radical Bio Med 190 (2022):202-215.PMID:35985562
- Phytomedicine 122 (2024):155091.PMID:37844378
- Phytomedicine 122 (2024):155091.PMID:37844378
- Cell Commun Signal 22.1 (2024):293.PMID:38802896
- Int J of Bio Macromol (2024):132103.PMID:38719011
- Sci Rep-Uk 15.1 (2025):1-17.PMID:40383858
- Int J Biol Macromol (2024):133667.PMID:38969038
- Int Immunopharmacol 158 (2025):114885.PMID:40383096
- Cell Mol Biol Lett 29.1 (2024):83.PMID:38822277
- Front Immunol 16 (2025):1569210.PMID:40396173
- Mater Today Bio (2025):101901.
- CANCER IMMUNOL RES (2025).PMID:40323348
- Int J Biol Macromol 224 (2023):797-809.PMID:36283555
- Cell Death Dis 12.4 (2021):408.PMID:33859186
- Cell Death Dis 13.3 (2022):233.PMID:35288538
- Int J Biol Macromol (2024):131928.PMID:38688339
- Sci Rep-Uk 15.1 (2025):1-14.PMID:40450025
- Sci Rep-Uk 15.1 (2025):1-16.PMID:40436970
- Cell Mol Life Sci 81.1 (2024):32.PMID:38214780
- Int. J. Biol. Macromol. (2025):143759.
- Int J Biol Macromol 306 (2025):141410.PMID:40010477
- Int J Biol Macromol (2025):140632.PMID:39909240
- Cell Death Dis 16.1 (2025):240.PMID:40175350
- Int J Biol Macromol 222 (2022):1789-1800.PMID:36191790
- Acs Appl Mater Inter (2025).PMID:40025657
- Int J Biol Macromol (2024):135979.PMID:39332550
- Sci China Life Sci (2025):1-11.PMID:39821161
- Acs Appl Mater Inter (2024).PMID:39448903
- Int J Biol Macromol (2025):140374.PMID:39880265
- Int. J. Biol. Macromol. (2025):142238.PMID:40112979
- Int J Biol Sci 21.2 (2025):614.PMID:39781455
- Int J Biol Sci 21.4 (2025):1837-1851.
- Int J Biol Macromol (2024):136818.PMID:39447809
- Nanoscale Horiz (2025).
- Int J Biol Macromol (2025):141146.PMID:39986528
- Int J Biol Macromol (2025):141767.
- Int J Biol Macromol(2023):125130.PMID:37263336
- Oncogene (2023):1-13.PMID:37138031
- Antivir Res 205 (2022):105381.PMID:35835291
- Acs Appl Mater Inter (2024).PMID:39320506
- Int J Biol Macromol (2024):137561.PMID:39537068
- Cell Death Dis 15.11 (2024):826.PMID:39543094
- Int J Biol Macromol (2024):135562.PMID:39276884
- Acs Appl Mater Inter (2025).PMID:39853325
- Int J Biol Macromol (2025):140602.PMID:39922352
- J Med Chem (2024).PMID:38315032
- Acta Pharmacol Sin (2025):1-15.PMID:39984622
- Int J Biol Macromol 211 (2022):128-139.PMID:35561854
- Biomed Pharmacother 129 (2020):110315.PMID:32554246
- Biomed Pharmacother 174 (2024):116470.PMID:38565061
- Front Immunol 13 (2022):902515.PMID:35874682
- Phytomedicine 130 (2024):155744.PMID:38763011
- Antioxid Redox Sign 37.13 (2022):867-886.PMID:35350885
- Phytomedicine (2025):156515.PMID:39983438
- J Med Chem (2025).PMID:40500735
- Biomed Pharmacother 177 (2024):117037.PMID:38959602
- Int J Biol Macromol 211 (2022):128-139.PMID:35561854
- Biomed Pharmacother 184 (2025):117907.PMID:39978030
- Cell Death Discov 9.1 (2023):415.PMID:37968255
- Front Cell Dev Biol 10 (2022):850708.PMID:35592246
- Phytomedicine (2025):156928.
- Cell Rep 44.3 (2025).PMID:39977268
- Biomed Pharmacother 168 (2023):115751.PMID:37879214
- Front Cell Dev Biol 9 (2021):696558.PMID:34447749
- Food Res Int (2025):115940.
- J Med Virol 96.11 (2024):e70049.PMID:39558699
- Front Immunol 13 (2022):986214.PMID:36341437
- J Mater Chem B 11.16 (2023):3740-3751.PMID:37067505
- Oncogenesis 11.1 (2022):21.PMID:35487890
- Ecotox Environ Safe 280 (2024):116579.PMID:38865940
- Cell Biol Toxicol 38.6 (2022):1121-1136.PMID:35348966
- Front Immunol 14 (2023):1111494.PMID:36817451
- Int J Nanomed (2024):13183-13199.PMID:39664759
- Nanoscale 16.17 (2024):8479-8494.PMID:38590261
- Stem Cell Res Ther 15.1 (2024) 385.PMID:39468662
- J Med Chem 65.4 (2021):2794-2808.PMID:33872498
- Bioeng Transl Med 8.3 (2023):e10475.PMID:37206234
- Biomed Pharmacother 122 (2020):109690.PMID:31786468
- Brit J Pharmacol (2024).PMID:38715438
- J Med Chem (2025).PMID:39842821
- Plos Pathog 17.3 (2021):e1009393.PMID:33684175
- Cell Death Discov 10.1 (2024):97.PMID:38402198
- Biomed Pharmacother 130 (2020):110566.PMID:32755793
- Cell Death Discov 10.1 (2024):7.PMID:38182573
- Eur J Med Chem 238 (2022):114458.PMID:35635946
- Fertil Steril (2023).PMID:37178109
- Oncogene (2025):1-15.PMID:39920289
- Front Nutr 9 (2022):812623.PMID:35419394
- Clin Epigenetics 14.1 (2022):1-12.PMID:35414117
- Phytother Res (2024).PMID:38558449
- Mater Today Bio 15 (2022):100264.PMID:35517578
- Front Cell Dev Biol 9 (2021):749210.PMID:34676217
- Ecotox Environ Safe 270 (2024):115813.PMID:38113798
- Free Radical Bio Med (2024).PMID:38367927
- Int J Nanomed 20 (2025):293-308.
- Virol Sin (2023).PMID:37331658
- Iscience (2025).
- Stem Cell Res Ther 16.1 (2025):1-18.PMID:40361250
- Stem Cell Res Ther 16.1 (2025):119.PMID:40050973
- Life Sci 308 (2022):120925.PMID:36057399
- J Med Chem (2024).PMID:39292661
- Wirel Commun Mob Com 2 (2022):3.PMID:36120596
- Npj Precis Oncol 9.1 (2025):112.PMID:40240582
- Ecotox Environ Safe 262 (2023):115161.PMID:37356398
- Biomed Pharmacother 188 (2025):118217.PMID:40435839
- Biomed Pharmacother 165 (2023):115261.PMID:37549461
- J Polym Environ (2025):1-16.
- Int J Nanomed (2025):4535-4550.PMID:4023652
- J Cell Physiol 238.1 (2023):195-209.PMID:36436184
- Biomed Pharmacother 162 (2023):114602.PMID:37018993
- AGING DIS 17.1 (2026):2.PMID:40072367
- Bba-Mol Basis Dis 1869.1 (2023):166571.PMID:36244542
- J. Environ. Chem. Eng. (2025):116286.
- Front Immunol 14 (2023).PMID:37662906
- Food Chem Toxicol 148 (2021):111972.PMID:33421461
- Bba Mol Basis Dis 1869.7 (2023):166725.PMID:37127173
- Embo Rep (2025):1-23. PMID:40185977
- Phytomedicine (2025):156860.
- Stem Cell Res Ther 10.1 (2019):1-14.PMID:31847890
- Plos Pathog 19.10 (2023):e1011702.PMID:37801439
- Inflamm Res 74.1 (2025):1-22.PMID:40439750
- Anal Chem 95.31 (2023):11664-11671.PMID:37495553
- Chem-Biol Interact (2025):111576.PMID:40456372
- Cell Death Discov 11.1 (2025):1-12.PMID:40456746
- J Med Virol 97.3 (2025):e70267.
- Life Sci 312 (2023):121092.PMID:36279968
- Biomed Pharmacother 158 (2023):114118.PMID:36527845
- Stem Cell Res Ther 15.1 (2024):1-18.PMID:39267160
- Phytomedicine (2025):156598.
- Ecotox Environ Safe 266 (2023):115571.PMID:37837696
- Biomed Pharmacother 179 (2024):117415.PMID:39265236
- J Gastroenterol (2023):1-14.PMID:37150773
- Phytomedicine (2024):155859.PMID:38972239
- Phytother Res 37.10 (2023):4639-4654.PMID:37394882
- Cell Death Discov 11.1 (2025):1-16.PMID:40399275
- Ecotox Environ Safe 233 (2022):113315.PMID:35189521
- Cancer Gene Ther 29.8-9 (2022):1228-1239.PMID:35115683
- Life Sci (2024):122483.PMID:38307238
- Biochem Pharmacol (2024):116044.PMID:38336157
- Int J Biochem Cell B 153 (2022):106317.PMID:36283586
- Mbio (2023):e01688-23.PMID:37882780
- Int J Mol Sci 23.22 (2022):14306.PMID:36430782
- Front Pharmacol 13 (2022):972397.PMID:36188529
- Cancer Cell Int 23.1 (2023):142.PMID:37468908
- Am J Chinese Med (2024):1-23.PMID:38490807
- Acs Biomater Sci Eng (2024).PMID:38446137
- Acs Biomater Sci Eng (2024).PMID:38445959
- Lab Invest 102.4 (2022):411-421.PMID:34775495
- Exp Neurol 359 (2023):114266.PMID:36336032
- Cells 11.23 (2022):3816.PMID:36497076
- J Agr Food Chem 70.50 (2022):15763-15775.PMID:36472370
- Front Cell Dev Biol 10 (2022):787730.PMID:35445026
- Int J Mol Sci 22.21 (2021):11893.PMID:34769322
- Aging (Albany NY) 13.17 (2021):21712.PMID:34518442
- Eur J Pharm Biopharm 188 (2023):89-99.PMID:36702198
- Apoptosis 28.3-4 (2023):414-431.PMID:36482019
- J Agr Food Chem 71.3 (2023):1593-1606.PMID:36634077
- Chinese Med J-Peking (2023):10-1097.PMID:36939243
- Aging (Albany NY) 15.12 (2023):5673.PMID:37387540
- Int Immunopharmacol 113 (2022):109342.PMID:36327871
- Front Oncol 12 (2022):858598.PMID:35321435
- Resp Res 24.1 (2023):216.PMID:37674165
- Front Pharmacol 13 (2022):927488.PMID:35935870
- Front Pharmacol 13 (2022):1043022.PMID:36588728
- Biomolecules 12.9 (2022):1283.PMID:36139122
- Cells 11.24 (2022):4094.PMID:36552856
- Mol Neurobiol 59.12 (2022):7075-7094.PMID:36083519
- Front Cell Dev Biol (2023):1161541.PMID:37325570
- Int J Mol Sci 24.22 (2023):16503.PMID:38003694
- J Agr Food Chem 71.1 (2022):546-556.PMID:36538589
- Colloid Surface B 222 (2023):113119.PMID:36621177
- Biochem Pharmacol 213 (2023):115592.PMID:37196680
- Am J Cancer Res 13.5 (2023):2135.PMID:37293170
- Mol Med 30 (2024):260.PMID:39707194
- J Agr Food Chem (2023).PMID:37922022
- Phytother Res (2023).PMID:37246830
- Faseb J 37.5 (2023):e22935.PMID:37086094
- J Enzym Inhib Med Ch 39.1 (2024):2288810.PMID:38059334
- Mar Life Sci Tech 4.3 (2022):373-383.PMID:37073164
- Biochem Pharmacol (2023):115632.PMID:37263300
- Front Pharmacol 13 (2022):946193.PMID:36091823
- Front Pharmacol 13 (2022):1015693.PMID:36210855
- Front Oncol 12 (2022):858598.PMID:35321435
- Front Pharmacol 14 (2023):1168393.PMID:37124212
- Mol Med 30.1 (2024):14.PMID:38254010
- Chinese Med J-Peking 136.05 (2023):556-564.PMID:36939243
- Front Cell Dev Biol 9 (2022):793793.PMID:35155447
- Phytother Res (2023).PMID:37086359
- Int Immunopharmacol 121 (2023):110413.PMID:37301119
- Int J Biochem Cell B 153 (2022):106317.PMID:36283586
- Front Oncol 13 (2023):1120103.PMID:36959804
- Int-Immunopharmacol 124 (2023):110809.PMID:37690240
- Int J Pharmaceut (2023):123142.PMID:37328119
- Front Oncol 12 (2022):770628.PMID:35372056
- Int J Mol Sci 23.24(2022):16045.PMID:36555684
- Cns Neurosci Ther (2023).PMID:37452510
- Front Pharmacol 13 (2022):819826.PMID:35645824
- J Transl Med 22.1 (2024):1-19.PMID:39107788
- Aging (Albany NY) 16.11 (2024):9625.PMID:38829771
- Aging (Albany NY) 13.3 (2021):4482.PMID:33495411
- Front Immunol 15 (2024):1455996.PMID:39376562
- MOL MED 31.1 (2025):126.PMID:40186145
- Neurotoxicol Teratol (2025):107500.PMID:40456500
- Int J Mol Med 55.3 (2025):1-17.
- Front Pharmacol 12 (2021):610158.PMID:33762939
- Ecotox Environ Safe 289 (2025):117511.PMID:39662457
- J Transl Med 22.1 (2024):1-17.PMID:39267037
- Front Immunol 15 (2024):1455996.PMID:39376562
- Apoptosis (2025):1-17.PMID:40347321
- Cancer Gene Ther (2021)1-12.PMID:33888871
- Mol Med 30.1 (2024):1-18.PMID:39266965
- Front Pharmacol 13 (2022):965131.PMID:36249790
- Int J Mol Med 55.3 (2025):1-14.PMID:39791203
- Biomater Adv (2025):214257.PMID:40023084
- Phytother. Res. 39.3 (2025):1355-1371.PMID:39764683
- Mol Neurobiol 58 (2021):1990-2004.PMID:33411316
- Biomacromolecules (2025).
- Front Immunol 15 (2024):1375781.PMID:39391314
- Jci insight 9.15 (2024):PMID:38935435
- Talanta (2025):127591.PMID:39847915
- J Invest Dermatol (2025).PMID:39892773
- Molecular Therapy Oncology 32.2 (2024).PMID:38948919
- Cell Death Discov 11.1 (2025):74.PMID:39988672
- Cell Mol Life Sci 81.1 (2024):265.PMID:38880863
- Liver Int 45.2 (2025):e16246.PMID:39821525
- Microbiol Res (2025):128183.
- Int J Mol Med 55.3 (2025):45.PMID:39791203
- J Mater Chem B (2024).PMID:39119756
- J Transl Med 22.1 (2024):1-15.PMID:39256832
- Jci Insight (2024). PMID:38935435
- Front Oncol 13 (2023):1132424.PMID:37251940
- J Nutr Biochem (2024):109678.PMID:38844080
- Cancer Cell Int 20 (2020):1-14.PMID:33292221
- Lab Chip (2024).PMID:38939907
- Front Immunol 16 (2025):1521965.PMID:40191190
- Front Immunol 16 (2025):1569210.
- J Agr Food Chem (2025).PMID:40152883
- Front Pharmacol 12 (2021):649398.PMID:34335241
- Int J Mol Med 55.4 (2025):1-16.PMID:39918010
- J Invest Dermatol (2025).PMID:39892773
- Int Immunopharmacol 132 (2024):111870.PMID:38547771
- Front Bioeng Biotech 12 (2024):1361682.PMID:38562665
- Cancer Cell Int 21 (2021):1-12.PMID:34281530
- Cancer Cell Int 24.1 (2024):123.PMID:38566075
- J Transl Med 23.1 (2025):131.PMID:39885513
- Apoptosis (2024):1-19.PMID:39097858
- Biomater Adv (2024):214069.PMID:39447240
- Stem Cell Transl Med (2024):szae020.PMID:38530131
- J Agr Food Chem (2025).PMID:40017402
- Mol Cell Proteomics (2025):100930.PMID:40024376
- Int Immunopharmacol 131 (2024):111922.PMID:38522137
- Am J Physiol-Cell Ph (2024).PMID:38525543
- Mol Med 31.1 (2025):1-15.PMID:40369420
- J Agr Food Chem (2025).PMID:39962827
- BREAST CANCER RES 27.1 (2025):61.PMID:40270028
- Microbiol Res (2024):127927.PMID:39393129
- Front Immunol 15 (2024):1375781.PMID:39391314
- Ecotox Environ Safe 287 (2024):117331.PMID:39547060
- Front. Immunol. 16 (2025):1556715.
- Bba-Mol Basis Dis (2024):167210.PMID:38704001
- Front Pharmacol 11 (2020):16.PMID:32140106
- Front Pharmacol 12 (2021):673263.PMID:34108880
- Mol Med 31.1 (2025):1-15.PMID:40405066
- Int J Mol Med 54.6 (2024):1-17.PMID:39364745
- Front Immunol 15 (2024):1455996.PMID:39376562
- Antioxid Redox Sign (2025).PMID:40455659
- J Ethnopharmacol (2024):119272.PMID:39716512
- Am J Physiol-Cell Ph (2024).PMID:39069827
- J oncol 2022 (2022).PMID:36385962
- Acs Biomater Sci Eng (2024).PMID:39164198
- CANCER CELL INT 25.1 (2025):1-24.PMID:40296031
- Biochem Pharmacol (2024):116688.PMID:39617210
- Life Sci (2024):123148.PMID:39447733
- Int Immunopharmacol 147 (2025):113871.PMID:39798467
- J Ethnopharmacol 310 (2023):116375.PMID:36934787
- Eur-J Pharmacol 958 (2023):176033.PMID:37673365
- J Cell Mol Med 24.7 (2020):4136-4149.PMID:32100443
- Food Funct. (2025).
- Biochem Pharmacol (2024):116438.PMID:39025409
- Int Immunopharmacol 141 (2024):112955.PMID:39163685
- J Ethnopharmacol (2024):118685.PMID:39127116
- Front Cell Dev Biol 12 (2024):1391873.PMID:39170916
- Int. J. Mol. Sci. 26.9 (2025):4325.
- J-Ethnopharmacol (2023):116729.PMID:37277081
- Sci Rep-Uk 13.1 (2023):361.PMID:36611103
- Bone Joint Res 13.12 (2024):763-777.PMID:39662502
- Bone Joint Res 13.12 (2024):763.PMID:39662502
- J. Drug Delivery Sci. Technol. (2025):106841.
- Glia (2024).PMID:39610064
- Acs Infect Dis 8.9 (2022):1851-1868.PMID:35948057
- J Nutr Biochem (2024):109780.PMID:39395694
- Bioengineering 9.9 (2022):433.PMID:36134979
- J Dermatol Sci 109.2 (2023):52-60.PMID:36858850
- Redox Rep 29.1 (2024):2398380.PMID:39284588
- Nutrients 17.5 (2025):923.PMID:40077793
- J Neurochem 164.5 (2023):643-657.PMID:36527420
- J Ethnopharmacol (2023):116731.PMID:37277084
- Pharmaceuticals 15.11 (2022):1435.PMID:36422564
- Pharmaceuticals 15.10 (2022):1271.PMID:36297383
- Molecules 27.23 (2022):8568.PMID:36500666
- Molecules 27.17 (2022):5698.PMID:36080465
- Front Endocrinol 13 (2022):998971.PMID:36147560
- J Oncol 2022 (2022).PMID:36213838
- ACS Appl. Nano Mater. (2025).
- Food Biosci (2025):106564.
- J Hepatocell Carcino (2023):863-881.PMID:37313303
- Chem-Biol Interact 382 (2023):110638.PMID:37473910
- Transl Oncol 56 (2025):102396.PMID:40239242
- Molecules 28.6 (2023):2426.PMID:36985397
- J Oncol 2022 (2022).PMID:36281290
- Front Oncol 12 (2022):996080.PMID:36483054
- J Ethnopharmacol 307 (2023):116193.PMID:36746295
- J Oncol 2022 (2022):6459029PMID:36199788
- Exp Neurol (2025):115252.PMID:40221008
- Mol Neurobiol (2024):1-18.PMID:38995443
- Invest Ophth Vis Sci 66.1 (2025):24-24.PMID:39792075
- Mol. Nutr. Food Res. (2025):e70053.PMID:40177855
- J. Ethnopharmacol. (2025):119727.PMID:40187623
- Molecules 27.21 (2022):7183.PMID:36364008
- Mol Neurobiol 59.8 (2022):4776-4790.PMID:35622272
- Mol Carcinogen 62.2 (2023):160-173.PMID:36222184
- Antivir Ther 30.3 (2025):13596535251345953.PMID:40434182
- J Oncol 2022 (2022).PMID:36213831
- Int Immunopharmacol 138 (2024):112533.PMID:38924868
- Cell Death Discov 8.1 (2022):85.PMID:35220396
- Environ Toxico (2024).PMID:38700384
- Am J Cancer Res 14.1 (2024):324.PMID:38323285
- Colloid Surface B (2025):114537.PMID:39879672
- Antimicrob Agents Ch 65.6 (2021):10-1128.PMID:33753340
- Int Immunopharmacol 107 (2022):108695.PMID:35305385
- J Dent (2024):105436.PMID:39488296
- Am J Physiol-Gastr L (2024).PMID:38742280
- Biotechnol J 19.1 (2024):2300453.PMID:37899497
- Int Immunopharmacol 108 (2022):108742.PMID:35378447
- Transl Oncol 52 (2025):102256.PMID:39733744
- Bioorg Chem 136 (2023):106556.PMID:37105002
- Radiother Oncol (2024):110686.PMID:39709027
- Aging 16.8 (2024):6773-6795.PMID:38643468
- J Ethnopharmacol 319 (2024):117205.PMID:37741473
- J Ethnopharmacol 319 (2024):117091.PMID:37634753
- Int Immunopharmacol 138 (2024):112625.PMID:38996666
- Antimicrob Agents Ch 65.6 (2021):10-1128.PMID:33753340
- Acs Appl Bio Mater (2024).PMID:38700956
- Cell Cycle 19.17 (2020):2139-2147.PMID:32779548
- J Ethnopharmacol (2024):117958.PMID:38395179
- Front Oncol 12 (2022):961653.PMID:36457504
- Front Oncol 12 (2022):1043161.PMID:36439418
- Toxicology (2024):154018.PMID:39608440
- J Ethnopharmacol (2024):118080.PMID:38521426
- J Cell Mol Med 28.6 (2024):e18223.PMID:38451046
- Burns Trauma 12 (2024):tkae001.PMID:38434722
- J Ethnopharmacol (2024):117985.PMID:38417600
- Cell Cycle 21.3 (2022):228-246.PMID:34965191
- Diabetol Metab Syndr 16.1 (2024):1-14.PMID:38317244
- Faseb J 38.7 (2024):e23591.PMID:38572579
- Front Endocrinol 13 (2022):657802.PMID:36263320
- Macromol Biosci (2024):2400003.PMID:38597147
- J Ethnopharmacol (2024):117865.PMID:38369066
- J Ethnopharmacol (2024):119073.PMID:39522846
- J Dent (2024):105436.PMID:39488296
- Drug Deliv Transl Re (2023):1-13.PMID:37130996
- Cell Cycle 21.8 (2022):835-850.PMID:35100080
- Macromol Biosci (2024):2400003.PMID:38597147
- J Agr Food Res (2025):101694.
- Aging (Albany NY) 16.8 (2024):6773.PMID:38643468
- Arch Toxicol (2025):1-17.PMID:40464975
- Transl Oncol 47 (2024):102042.PMID:38924847
- J Drug Deliv Sci Tec 104 (2025) 106577.
- Commun Biol 8.1 (2025):71.PMID:39820362
- Bioorg Chem (2025):108667.
- Bioorg Chem (2025):108656.
- Int J Mol Med 52.5 (2023):1-14.PMID:37800609
- Drug Des Dev Ther (2025):281-302.PMID:39845152
- Microorganisms 11.5 (2023):1268.PMID:37317242
- J Virol 97.11 (2023):e00719-23.PMID:37929962
- Sci Rep-Uk 13.1 (2023):14339.PMID:37658227
- Molecules 27.14 (2022):4603.PMID:35889477
- Mol Neurobiol (2025):1-16.PMID:40402409
- Int J Mol Sci 26.10 (2025):4953.PMID:40430095
- Int Immunopharmacol 159 (2025):114886.PMID:40424656
- Cns Neurosci Ther 31.5 (2025):e70437.PMID:40400263
- Clin Immunol (2024):110308.PMID:39002794
- Mol Carcinogen (2023).PMID:37750597
- Cell Signal 113 (2024):110962.PMID:37931691
- Arch Toxicol (2024):1-16.PMID:38971901
- J Virol (2023):e00549-23.PMID:37222617
- Radiother Oncol 203 (2025):110686.PMID:39709027
- Virulence 16.1 (2025):2500493.PMID:40521898
- Int Immunopharmacol 138 (2024):112533.PMID:38924868
- Drug Des Dev Ther (2025):5123-5141.
- J Cell Mol Med 26.13 (2022):3783-3796.PMID:35656815
- Life Sci 254 (2020):117793.PMID:32416164
- Invest Ophth Vis Sci 63.6 (2022):13-13.PMID:35695808
- Cell Signal (2023):110987.PMID:38029946
- Aging (Albany NY) 15.23 (2023):14292.PMID:38059876
- Mol Nutr Food Res (2024):2300912.PMID:38847553
- J Ethnopharmacol (2025):120157.PMID:40532799
- Int Immunopharmacol 138 (2024):112539.PMID:38936054
- Int Immunopharmacol 138 (2024):112559.PMID:38955028
- Environ Toxicol (2023).PMID:38069587
- Eur J Pharmacol 983 (2024):176995.PMID:39277096
- Zool Res 45.5 (2024):1001.PMID:39147715
- Front Oncol 14 (2024):1471109.PMID:39582546
- Sci Rep-Uk 14.1 (2024):27063.PMID:39511266
- Heliyon 8.11 (2022).PMID:36353164
- J Neuro Oncol (2023):1-16.PMID:37351767
- J Inflamm Res (2024):8009-8026.PMID:39507268
- Sci Rep-Uk 14.1 (2024):25636.PMID:39465338
- J Cell Mol Med 28.20 (2024):e70163.PMID:39462268
- Front Oncol 14 (2024):1440906.PMID:39346724
- J Inflamm Res (2024):8009-8026.PMID:39507268
- Zool Res 45.5 (2024).PMID:39147715
- J Biochem Mol Toxic 38.4 (2024):e23675.PMID:38488158
- Environ Toxicol (2024).PMID:39400944
- Endocrine (2024):1-14.PMID:38378893
- Cancer Biol Ther 25.1 (2024):2355705.PMID:38778753
- Bmc Complement Med 24 (2024).PMID:38783288
- Oral Dis (2024).PMID:38168877
- Gerontology (2024).PMID:38168028
- J Gen Virol 105.1 (2024):001944.PMID:38180473
- Endocrine (2024).PMID:38191984
- Cytokine 176 (2024):156507.PMID:38244240
- Pediatr Res (2024):1-10.PMID:38658662
- J Inflamm Res (2024):10533-10546.PMID:39659750
- Heliyon (2024).PMID:38707472
- Rsc Adv 14.18 (2024):12864-12872.PMID:38650686
- Toxicol Appl Pharm (2023):116794.PMID:38142782
- J Biochem Mol Toxic 38.1 (2024):e23544.PMID:37815058
- J Cancer 15.5 (2024):1397.PMID:38356722
- J Pharmacol Exp Ther 388.3 (2024):827-845.PMID:38262745
- Bmc Genomics 25.1 (2024):1-13.PMID:38395755
- Bmc Cancer 24.1 (2024):465.PMID:38622522
- Acta Derm-Venereol 104 (2024).PMID:38590175
- Bmc Complement Med 24.1 (2024):1-16.PMID:38580999
- Microbiol Spectr (2024):e04142-23.PMID:38421191
- Front Pharmacol 15 (2024):1453938.PMID:39654626
- Gene (2024):148399.PMID:38518902
- Bmc Cancer 24.1 (2024):1-16.PMID:38475728
- J Immunother (2024):10-1097.PMID:38847148
- Gene (2023):148050.PMID:38042211
- Toxicol Lett 379 (2023):20-34.PMID:36905973
- Bba-Mol Basis Dis (2024):167467.PMID:39159699
- Bmc Chem 18.1 (2024):1-11.PMID:39396055
- Metabolomics 20.5 (2024):112.PMID:39369160
- Sci Rep-Uk 14.1 (2024):21680.PMID:39289451
- Eur J Pharmacol (2024):176995.PMID:39277096
- Mol Cells 46.4 (2023):231-244.PMID:36625319
- Front Pharmacol 15 (2024):1424624.PMID:25956975
- Mol Cell Biochem (2024):1-15.PMID:39179754
- Sci Rep-Uk 14.1 (2024):19292.PMID:39164313
- Sci Rep-Uk 14.1 (2024):18929.PMID:39147857
- Rsc Adv 13.32 (2023):22367-22374.PMID:37497090
- Aging (Albany NY) 16.14 (2024):11339.PMID:39029955
- Sci Rep-Uk 14.1 (2024):14438.PMID:38910141
- Epigenetics-Us 18.1 (2023):2254976.PMID:37691391
- Cancer Biol Ther 25.1 (2024):2325126.PMID:38445610
- Front Pharmacol 15 (2024):1397761.PMID:39104391
- Eur J Pharm Biopharm (2024):114417.PMID:39013493
- Rsc Adv 13.33 (2023):23010-23020.PMID:37529353
- Langmuir 39.39 (2023):14162-14172.PMID:37722015
- Bba-Mol Basis Dis (2024):167354.PMID:39004378
- Brain Res Bull (2024):111165.PMID:39667505
- Biomed Mater 18.6 (2023):065026.PMID:37852221
- J Biol Chem (2024):107499.PMID:38944125
- J Steroid Biochem (2023):106429.PMID:38035949
- Toxicol Sci (2024):kfae054.PMID:38636494
- Oncol Rep 53.2 (2025):1-9.PMID:39670302
- Front Genet 13 (2022):959109.PMID:36147489
- Front. Pharmacol. 16 (2025):1545498.
- Int J Mol Med 49.3 (2022):1-11.PMID:35014672
- Cell Death Dis 16.1 (2025):287.PMID:40223081
- Sci Rep-Uk 15.1 (2025):11639.PMID:40185911
- Frontiers in Pharmacology 16 (2025):1547685.
- Front Pharmacol 16 (2025):1562839.PMID:40183104
- Front Pharmacol 16 (2025):1517221.
- Bmc Cancer 22.1 (2022):1-14.PMID:35387643
- J Oncol 2022 (2022).PMID:35518787
- Hum Cell 35.4 (2022):1140-1158.PMID:35581525
- Brain Res Bull 187 (2022):1-10.PMID:35738501
- Metab Brain Dis 37.7 (2022):2349-2362.PMID:35779150
- J. Biomed. Mater. Res. Part A 113.3 (2025):e37893.PMID:40059713
- Eur J Pharmacol (2025):177497.PMID:40058753
- Hypertens Res 45.4 (2022):650-664.PMID:35082376
- Mol Cell Biochem (2025):1-20.PMID:40032749
- Pharm Biol 60.1 (2022):1436-1448.PMID:35938494
- Front Pharmacol 16 (2025):1536091.PMID:40041493
- J Cancer 11.5 (2020):1162.PMID:31956362
- J Biochem Mol Toxice 36.11 (2022):e23200.PMID:35997646
- Arch Biochem Biophys (2025):110352.PMID:39978618
- Mol Immunol 152 (2022):27-34.PMID:36244215
- Neurotox Res 40.6 (2022):1895-1901.PMID:36223054
- Metab Brain Dis 38.1 (2023):255-267.PMID:36445630
- Front Pharmacol 15 (2025):1534772.PMID:39911826
- Faseb J 39.2 (2025):e70314.PMID:39825721
- Eur J Pharmacol (2025):177343.PMID:39900330
- Thorac Cancer 13.5 (2022):761-770.PMID:35098684
- Cell Signal (2025):111815.PMID:40258578
- Bmc Med Genomics 15.1 (2022):1-19.PMID:36503492
- J Ethnopharmacol 278 (2021):114275.PMID:34087404
- J Ethnopharmacol 259 (2020):112956.PMID:32442587
- J Inflamm Res (2025):7401-7417.PMID:40503490
- Oncotargets Ther (2020):11301-11313.PMID:33177838
- Sci Rep-Uk 10.1 (2020):20623.PMID:33244088
- Toxicol Appl Pharm 410 (2021):115361.PMID:33285147
- Oncotargets Ther (2020):2511-2522.PMID:32280236
- Front Pharmacol 16 (2025):1565713.PMID:40474975
- Poultry Sci (2025):105339.
- Biol 14.6 (2025):594.
- Acta Diabetol 58.9 (2021):1251-1267.PMID:33907874
- Toxicol Appl Pharm 422 (2021):115559.PMID:33961903
- Oncol Rep 46.1 (2021):1-12.PMID:34080652
- Front Genet 13 (2022):932973.PMID:36118847
- Sci Rep-Uk 15.1 (2025):13282.PMID:40247060
- Sci. Rep. 15.1 (2025):15556.PMID:40319036
- Probiotics Antimicro (2025):1-16.PMID:40366615
- Bmc Cancer 21.1 (2021):1-15.PMID:34130645
- Front Toxicol 7 (2025):1598185.PMID:40376450
- J. Funct. Foods 128 (2025):106822.PMID:39486993
- Transl Oncol 14.12 (2021):101219.PMID:34555726
- J. Cell. Mol. Med. 29.8 (2025):e70476.PMID:40275529
- Oncol Rep 46.6 (2021):1-12.PMID:34643248
- Front. Oncol. 15 (2025):1536473.PMID:40303992
- Food Chem. Toxicol. (2025):115488.PMID:40288513
- Bmc Cancer 21.1 (2021):1181.PMID:34740322
- Am J Transl Res 13.10 (2021):11255.PMID:34786056
- Inflammation 45.1 (2022):372-386.PMID:34792688
- Mol Immunol 153 (2023):126-134.PMID:36495817
- Spectrochim Acta A 237 (2020):118387.PMID:32416513
- Arthritis Res Ther 27.1 (2025):12.PMID:39838477
- Ann Transl Med 10.24 (2022).PMID:36660714
- Sci Rep-Uk 15.1 (2025):794.
- Molecules 30.3 (2025):549.PMID:39942654
- Neuroscience (2023).PMID:37286157
- Mol Cell Biochem (2023):1-11.PMID:36939994
- Bioorgan Med Chem 82 (2023):117234.PMID:36906964
- Bioorgan Med Chem 72 (2022):116994.PMID:36087428
- Biotechnol Genet Eng (2023):1-15PMID:37018431
- Oncol Rep 53.2 (2025):1-9.PMID:39670302
- Brain Res Bull (2025):111245.PMID:39924054
- Biotechnol Genet Eng (2023):1-17.PMID:36946780
- Environ Toxicol (2023).PMID:37052456
- Neurochem Res (2023):PMID:37127800
- Bioscience Rep 43.5 (2023):BSR20230028.PMID:37083601
- J Endocrinol Invest (2025):1-16.PMID:39786708
- Heliyon (2024):.PMID:37461802
- Mol Biotechnol 64.12 (2022):1367-1375.PMID:35639278
- Bioengineered 13.4 (2022):10335-10349.PMID:35435110
- Tissue Cell (2025): 102903.
- Curr Pharm Biotechno 23.8 (2022):1101-1108.PMID:34463226
- Bmc Cancer 24.1 (2024):1-21.PMID:39085784
- MED ONCOL 42.4 (2025):1-13.PMID:40072654
- J ORTHOP SURG RES 20.1 (2025):443.PMID:40319252
- Discov Oncol 15.1 (2024):309.PMID:39060620
- J Orthop Surg Res 18.1 (2023):910.PMID:38031136
- Tissue Cell (2024):102477.PMID:39018712
- Bosn J Basic Med Sci 20.3 (2020):357.PMID:31733641
- Bioengineered 13.5 (2022):11987-12002.PMID:35603556
- Microb Pathogenesis (2025):107720.PMID:40403987
- Kaohsiung J Med Sci (2025):e70031.PMID:40387410
- Mol. Med. Rep. 31.5 (2025):1-12.PMID:40145553
- WORLD J GASTRO ONCOL 17.3 (2025):102187.PMID:40092949
- Bba-Gen Subjects (2024):130628.PMID:38642815
- Future Microbiol 0 (2024).PMID:38629914
- J Pharm Pharmacol 71.9 (2019):1412-1420.PMID:31282010
- Chem Biol Drug Des 104.1 (2024):e14580.PMID:39031936
- Int J Chronic Obstr (2023):919-932.PMID:37229441
- Virus Res 313 (2022):198748.PMID:35304133
- Plos One 19.12 (2024):e0316362.PMID:39724098
- N S Arch Pharmacol (2023):1-13.PMID:37160483
- J Mol Neurosci 74.1 (2024):30.PMID:38478195
- Bioengineered 12.2 (2021):11936-11946.PMID:34784841
- Bioengineered 13.1 (2022):1518-1529.PMID:34986734
- Gene 932 (2025):148898.PMID:39209182
- Bioengineered 13.1 (2022):455-468.PMID:34967265
- Hum Mol Genet (2024):ddae122.PMID:39216871
- J Appl Toxicol 42.5 (2022):830-840.PMID:34708435
- J Mol Neurosci 72.2 (2022):433-440.PMID:35023001
- Chem Biol Drug Des 105.4 (2025):e70111.PMID:40247760
- CURR ISSUES MOL BIOL 47.5 (2025):314.
- FEBS Open Bio 12.1 (2022):146-153.PMID:34644456
- Curr Stem Cell Res T 19.7 (2024):1021-1028.PMID:37861049
- Mol Carcinogen (2025).PMID:40222040
- Bioengineered 12.1 (2021):8698-8708.PMID:34697986
- Biomarkers (2024):1-12.PMID:38568742
- METABOLITES 15.5 (2025):290.
- Cell Transplant 33 (2024).PMID:38334047
- Chem Biol Drug Des 99.6 (2022):828-838.PMID:35184389
- Bioengineered 13.3 (2022):6650-6664.PMID:35230214
- Bioengineered 13.3 (2022):6807-6818.PMID:35246020
- Bioengineered 12.1 (2021):5985-5995.PMID:34477479
- Luminescence 39.1 (2024):e4671.PMID:38286599
- Bioengineered 12.1 (2021):6687-6702.PMID:34516362
- Biochem Bioph Res Co (2025):151972.
- Acta Bioch Bioph Sin 56.5 (2024):776.PMID:38495003
- ONCOL LETT 29.6 (2025):1-12
- Planta Med 89.05 (2023):493-507.PMID:35716667
- Eur Rev Med Pharmaco 23.5 (2019).PMID:30915737
- Shock (2023):10-1097.PMID:37988072
- Discov Oncol 15.1 (2024):280.PMID:39004679
- Bmc Oral Health 24 (2024):1421.PMID:39574093
- Toxicol Appl Pharm (2025):117232.PMID:39832565
- Dis Markers 2022 (2022).PMID:35845132
- Microb Pathogenesis (2024):107055.PMID:39442820
- Mol Med Rep 28.5 (2023):1-12.PMID:37732544
- Pharmacology 108.2 (2023):166-175.PMID:36724745
- S Afr J Bot 177 (2025):445-456.
- Mol Med Rep 31.3 (2025):1-10.
- Eur J Med Res 30.1 (2025):54.PMID:39865289
- J Tradit Chin Med 43.4 (2023):744.PMID:37454259
- Tissue Cell 80 (2023):102001.PMID:36565506
- Technol Cancer Res T 19 (2020).PMID:33111613
- Oncol Res 32.6 (2024):1129.PMID:38827325
- Carcinogenesis (2025):bgaf030.
- Comput Biol Chem (2025):108540.PMID:40483799
- Mol Cell Biochem 473 (2020):179-192.PMID:32627113
- Luminescence 40.2 (2025):e70095.PMID:39924164
- Genomics (2025):111009.PMID:39864635
- Cell Stress Chaperon 25 (2020):955-968.PMID:32572784
- Front Med-Lausanne 11 (2025):1534120.PMID:39882536
- Exp Eye Res (2025):110492.
- Pathogens 14.2 (2025):182.
- J Orthop Surg Res 19.1 (2024):709.PMID:39487541
- Dis Markers 2022 (2022).PMID:35968506
- Toxicol Appl Pharm (2025):117264.PMID:39952301
- J Orthop Surg Res 19.1 (2024):709.PMID:39487541
- Clinics 77 (2022).PMID:35662010
- Acta Bioch Bioph Sin 56.10 (2024):1473.PMID:38826133
- Acta Bioch Bioph Sin 56.10 (2024):1473.PMID:38826133
- Shock 59.3 (2023):426-433.PMID:36609531
- Plos One 20.6 (2025):e0317930.PMID:40455747
- Mol Biol Rep 51.1 (2024):1-13.PMID:39422836
- Mol Brain 18.1 (2025):1-23.PMID:39962509
- Luminescence 40.2 (2025):e70116.PMID:39914999
- CURR DRUG DELIV (2025).PMID:39976030
- Dis Markers 2022 (2022).PMID:35845132
- Int J Chronic Obstr (2025):623-639.
- N-S Arch Pharmacol (2024):1-21.PMID:39549064
- Thorac Cancer 14.9 (2023):815-826.PMID:36737402
- Toxicol Lett (2025).PMID:40412772
- J Sci Food Agr (2024).PMID:39394871
- J Integr Complement (2025).PMID:40459144
- J Mech Behav Biomed (2025):106963.
- Mol Cell Biochem 476 (2021):1387-1400.PMID:33389498
- Cell Mol Biol Lett 24.1 (2019):1-14.PMID:31889958
- J Mol Histol (2024):1-8.PMID:38758519
- Bioengineered 12.2 (2021):11451-11460.PMID:34895055
- Mol Biotechnol (2024):1-15.PMID:38771419
- Mol Biol Rep 52.1 (2025):212.PMID:39921807
- Clin Exp Pharmacol P 50.11 (2023):903-913.PMID:37635387
- Pathogens 14.2 (2025):182.
- Shock (2023):10-1097.PMID:37179246
- Toxicon (2025):108293.PMID:39999927
- Hematology 28.1 (2023):2220522.PMID:37272581
- Immunobiology (2024):152832.PMID:38943814
- Particul Sci Technol (2025):1-13.
- Pediatr Surg Int 40.1 (2024):63.PMID:38431920
- Transl Cancer Res 14.5 (2025):2594.PMID:40530117
- Cancer Biomark 42.5 (2025):18758592241306263.
- Vet Parasitol (2024):110284.PMID:39126893
- J Int Med Res 49.3 (2021).PMID:33730930
- Adv Clin Exp Med 30.10 (2021):1031-1041.PMID:34329545
- Chin J Integr Med (2024):1-11.PMID:38941045
- Oncol Res 33.1 (2024):103.PMID:39735676
- J Interf Cytok Res (2025).PMID:40512525
- Mol Cell Probe (2025):102031.
- Immunobiology (2025):153090.
- Curr Cancer Drug Tar 24.9 (2024):975-986.PMID:38204265
- Pathol Res Pract 217 (2021):153284.PMID:33249400
- Exp Ther Med 20.4 (2020):3290-3300.PMID:32855700
- Chem Biodivers 18.4 (2021):e2001007.PMID:33624427
- Genes Genom (2023):1-15.PMID:37318704
- J Int Med Res 48.4 (2020).PMID:31891283
- Biochem Genet (2023):1-18.PMID:37148331
- Biochem Bioph Res Co (2025):152196.PMID:40516308
- World J Oncol (2024):.
- Comb Chem High T Scr (2024).PMID:35894460
- Vet Microbiol (2025):110495.
- Chem Biodivers 18.4 (2021):e2001007.PMID:33624427
- Open Life Sci. 20.1 (2025):20221056.
- Chin J Integr Med (2025):1-10.PMID:40338446
- Clin Hemorheol Micro (2023):1-15.PMID:37212088
- Clin Cosmet Inv Dent (2022):87-94.PMID:35411190
- J Obstet Gynaecol Re PMID:39169658
- Balk Med J 41.6 (2024):491.PMID:39319820
- Clin Cosmet Inv Dent (2022):87-94.PMID:35411190
- J Obstet Gynaecol Re PMID:39169658
- Cell J 24.1 (2022):15.PMID:35182060
- Endocr Metab Immune 24.1 (2024):153-162.PMID:37150983
- Aesthet Plast Surg (2023):1-11.PMID:38148357
- Cell Mol Biol 70.2 (2024):161-168.PMID:38430026
- Fitoterapia (2024):106245.PMID:39393711
- TRANSL ANDROL UROL 14.2 (2025):36078-36378.PMID:40114844
- Neuroreport 33.10 (2022):413.PMID:35623086
- PeerJ 13 (2025):e19083.
- Pediatr Surg Int 39.1 (2022):54.PMID:36534156
- J Int Med Res 48.4 (2020).PMID:31891283
- Discov Oncol 14.1 (2023):1-10.PMID:37907650
- Ann Hepatol 27.4 (2022):100700.PMID:35338010
- Bmc Nephrol 24.1 (2023):314.PMID:37884904
- Acta Histochem 125.8 (2023):152097.PMID:37813066
- Curr Mol Pharmacol 17.1 (2024).PMID:36946474
- Anticancer Res 43.8 (2023):3411-3418.PMID:37500147
- J Membrane Biol 253 (2020):43-55.PMID:31820013
- J Toxicol Sci 48.8 (2023):469-479.PMID:37532580
- Anim Biotechnol (2023):1-12.PMID:36756956
- Balk Med J 41.6 (2024).PMID:39319820
- Cell J 25.8 (2023):554.PMID:37641417
- Am J Transl Res 15.7 (2023):4873.PMID:37560235
- Bmc Pediatr 25.1 (2025):42.PMID:39825267
- J Cardiothorac Surg 18.1 (2023):222.PMID:37420297
- AESTHET PLAST SURG (2025):1-15.PMID:40180642
- Cell Mol Biol 69.12 (2023):223-231.PMID:38063090
- Cell J 24.1 (2022):15.PMID:35182060
- Vet Parasitol 331 (2024):110284.PMID:39126893
- Int J Gen Med (2022):3199-3213.PMID:35342302
- N S Arch Pharmacol (2023):1-13.PMID:37052642
- Int J Gen Med (2022):3199-3213.PMID:35342302
- J Asian Nat Prod Res 24.11 (2022):1078-1085.PMID:34935551
- Am J Transl Res 14.10 (2022):6964.PMID:36398260
- J Cardiothorac Surg 20.1 (2025):53.PMID:39794868
- Iran J Immunol (2023).PMID:37865874
- Crit Rev Eukar Gene (2025).
- J Herbs Spices Med P (2025):1-16.
- J Craniofac Surg 33.4 (2022):1250-1254.PMID:36041089
- Transl Cancer Res 13.4 (2024).PMID:38737693
- Transpl P 52.1 (2020):398-405.PMID:31928781
- Acs Appl Nano Mater (2024).
- Am J Cancer Res (2024):2852-2867.
- scienceasia (2024).
- Biomedicines 12.11 (2024):2543.
- New J Chem 48.11 (2024):5068-5073.
- Green Process Synth 13.1 (2024):20240050.
- bioRxiv (2025):2025-01.
- J Transl Med 22.1 (2024) 1-16.
- Bioact Mater 36 (2024):524-540.
- Biomed Pharmacother 179 (2024):117370.
- Heliyon (2024).
- Bioact Mater 44 (2025):220-235.
- Heliyon (2024).
- Biosens Bioelectron (2024):116875.
- Carbohyd Polym (2024):122897.
- Phytother Res 34.4 (2020):836-845.
- Drug Resist Update (2024):101161.
- Front Pharmacol 15 (2024):1384141@0.jpg
- Front Pharmacol 15:1453938.
- Mol Ther Nucl Acids (2020).
- Int J Mol Sci 25.20 (2024):11184.
- J Hazard Mater (2024) 135735.
- J Integr Med-Jim (2024).
- Antioxidants 13.11 (2024) 1320.
- Front Bioeng Biotech 12 (2024):1460870.
- Antioxidants-Basel 13.11 (2024):1289.
- Netw Model Anal Hlth 13.1 (2024):1-15.
- bioRxiv (2024) 2024-10.
- Ecotox Environ Safe 286 (2024):117233.
- Gene (2024) 149056.
- ACS Appl. Eng. Mater. (2025).
- Small (2024):2402308.PMID:3911
- Int J Bioprinting (2024).
- Int J Biol Sci 20.12 (2024):4713-4730.
- Viruses 16.8 (2024):1239.
- Drug Dev Res 81.4 (2020):437-443.PMID:31904108
- Faseb J 38.15 (2024):e23876.
- Int J Mol Sci 25.15 (2024):8340.
- Front Oncol 10 (2020):387.
- J Tradit Compl Med (2024).
- Indian J Pharm Sci 86.3 (2024).
- Heliyon (2024).
- J Transl Med 22.1 (2024):1-16.
- J Nutr Biochem (2024):109769.PMID:9276944
- Phytomedicine (2024):156091.
- Cell Biomaterials (2025):
- Acs Omega (2024).
- Nutrients 16.14 (2024):2346.
- Int J Mol Sci 25.19 (2024):10339.
- Adv Funct Mater 29.50 (2019):1906690.
- Bioact Mater 43 (2025):32-47.
- Acs Appl Mater Inter (2024).PMID:39167710
- J Hazard Mater (2024):136113.
- hLife (2024).
- Bioorg Chem (2024):107731.
- Drug Des Dev Ther (2024):5841-5851.
- J Funct Foods 123 (2024):106621.
- Chem Eng J (2024):156542.
- Nutrients 16.14 (2024):2314.
- Rev Bras Farmacogn (2024):1-7.
- Mater Express 10.1 (2020):102-111.
- Toxins 16.7 (2024):319.
- Pharmaceuticals-Base 17.10 (2024):1295.
- J Pharm Anal (2024):101053.
- Int J Mol Sci 25.19 (2024):10496.
- Mater Res Express 7.2 (2020):025401.
- Adv Funct Mater (2024):2402663.
- J King Saud Univ Sci 32.7 (2020):2912-2919.
- Trop J Pharm Res 22.9 (2023):1857-1862.
- Int J Mol Sci 24.19 (2023):15003.PMID:37834451
- Oxid Med Cell Longev 2022 (2022).PMID:35936222
- Biochip J (2023):1-10.
- Dis Markers 2022 (2022).
- Pharmaceuticals-Base 16.10 (2023):1482.PMID:37895953
- Mol Cell Toxicol (2023):1-10.
- Discov Oncol 13.1 (2022):71.PMID:35941331
- Int J Mol Sci 23.16 (2022):9014.PMID:36012290
- Viruses 14.8 (2022):1783.PMID:36016405
- Trop J Pharm Res 22.9 (2023):1779-1784.
- Phytomedicine 105 (2022):154330.PMID:35905566
- Sensor Actuat B Chem 394 (2023):134396.
- Int J Mol Sci 24.21 (2023):15545.PMID:37958531
- J Oncol 2022 (2022).PMID:36059813
- Molecules 27.16 (2022):5337.PMID:36014573
- Mol Cell Oncol 10.1 (2023):2238873.PMID:37649964
- Cancers 14.19 (2022):4645.PMID:36230568
- Microorganisms 11.9 (2023):2258.PMID:37764102
- Phytother Res 37.2 (2023):477-489.PMID:36199227
- J-Ethnopharmacol 318 (2024):116961.PMID:37541404
- J Oncol 2022 (2022).PMID:35909906
- Front Immunol 14 (2023).PMID:37901236
- Pharmaceutics 14.10 (2022):2157.PMID:36297592
- Int J Biol Sci 20.2 (2024):433-445.PMID:37379961
- Front Pharmacol 13 (2022):899775.PMID:35571096
- Front Cell Infect Mi 12 (2022):423.PMID:35573770
- Clin Transl Disc 2.2 (2022):e49.
- Acta Trop 232 (2022):106519.PMID:35584779
- Adv Funct Mater (2023):2309690.
- Oncol Rep 51.2 (2024):1-13.PMID:38131250
- Biomed Pharmacother 170 (2024):116042.PMID:38118351
- Cytotechnology 74.3 (2022):421-432.PMID:35733701
- Int J Gen Med (2023):5817-5839.PMID:38106976
- Pharmaceutics (2023) 15(12):2713.
- Vaccines-Basel 11.11 (2023):1678.PMID:38006011
- Phytomedicine 103 (2022):154199.PMID:35679793
- Foods 11.11 (2022):1575.PMID:35681325
- Food Sci Nutr (2023).
- Oxid Med Cell Longev 2022 (2022).PMID:35770048
- Biocell 47.8 (2023).
- Ann Hematol (2023):1-12.
- Mater Today Adv 15 (2022):100271.
- Transl Cancer Res 11.6 (2022):1781.PMID:35836521
- Crit Rev Eukar Gene 34 (2024).
- Carbohyd Polym 295 (2022):119878.PMID:35989018
- Int J Nanomed (2023):5423-5440.PMID:37767196
- Biomaterials Advances 142 (2022):213165.PMID:36341744
- Adv Funct Mater (2024):2305827.
- Curr Issues Mol Biol 45.5 (2023):4017-4034.PMID:37232725
- Int J Pept Res Ther 29.2 (2023):19.
- ACS omega 8.30 (2023):27146-27155.PMID:37546678
- Plos Pathog 19.4 (2023):e1011305.PMID:37053288
- Int J Mol Sci 24.7 (2023):6279.PMID:37047251
- J Inflamm Res (2023):1045-1057.PMID:36936349
- View-China 4.2 (2023):20220052.
- Iscience 26.9 (2023).PMID:37670789
- Front Microbiol 14 (2023).PMID:37645224
- bioRxiv (2023):2023-05.PMID:37292976
- J Thorac Dis 15.4 (2023):2198.PMID:37197522
- Mol Med 29.1 (2023):6.PMID:36647009
- Ceram Int 49.15 (2023):25953-25963.
- Nanophotonics 11.22 (2022):5089-5100.
- Colloid Surface B 228 (2023):113364.PMID:37290201
- Oncol Lett 26.1 (2023):1-17.
- Adv Funct Mater (2023):2214998.
- Cancer Res Commun 2.6 (2022):471-482.PMID:36338546
- Acs Appl Polym Mater 4.10 (2022):7014-7024.
- bioRxiv (2022):2022-04.
- Front Mar Sci 9 (2022):861117.
- Oxid Med Cell Longev 2023 (2023).PMID:36778205
- Aging (Albany NY) 15.12 (2023):5713PMID:37390121
- Cancers 14.21 (2022):5218.PMID:36358640
- Acs Appl Bio Mater 6.1 (2023):74-82.PMID:36603189
- Exp Ther Med 26.5 (2023):1-15.PMID:37869642
- J Agr Food Chem 70.44 (2022):14220-14234.PMID:36300841
- Cells 11.22 (2022):3580.PMID:36429010
- Chem Eng J 474 (2023):145358.
- Cell Mol Biol 69.5 (2023):44-50.PMID:37571902
- Mol Cell Toxicol (2022):1-11.
- Histol Histopathol 35.9 (2020):919-927.PMID:32282924
- Biocell 47.8 (2023).
- Vaccines Basel 11.7 (2023):1190.PMID:37515006
- Poultry Sci 102.10 (2023):102946.PMID:37542939
- Poultry Sci 102.4 (2023):102511.PMID:36805396
- Mater Today Chem 27 (2023):101331.
- Int J Mol Sci 24.2 (2023):1243.PMID:36674761
- Exp Ther Med 25.2 (2023):1-10.PMID:PMID_36684652
- J-Ethnopharmacol 318 (2024):116969.PMID:37516391
- Aquaculture 564 (2023):739060.
- Int Immunopharmacol 123 (2023):110709.PMID:37515849
- Drug Develop Res 84.2 (2023):312-325.PMID:36658741
- Crystals 13.7 (2023):1067.
- Sensor Actuat B-Chem 381 (2023):133437.
- Appl Entomol Zool 58.4 (2023):303-313.
- Int J Mol Sci 23.8 (2022):4128.PMID:35456945
- Bioengineered 13.4 (2022):9145-9155.PMID:35363601
- Chin Herb Med (2024).
- Cells 13.9 (2024):728.
- Phytomedicine (2024):155656.
- Open Med-Warsaw 16.1 (2021):558-569.PMID:33869778
- Phytomedicine 86 (2021):153565.PMID:33945919
- Mol Biotechnol 63.8 (2021):686-693.PMID:33977498
- Compos Part B-Eng 220 (2021):108984.PMID:35988999
- Diabet Metab Synd Ob (2021):2255-2268.PMID:34045875
- Acs Appl Bio Mater 4.6 (2021):5026-5032.
- Exp Eye Res 209 (2021):108642.PMID:34058232
- Pharmaceuticals-Base 17.4 (2024):515.
- J Inflamm Res 14 (2021):2445.
- Adv Funct Mater (2024):2400489
- HemaSphere 5.7 (2021).PMID:34136753
- Applied Sciences 14.8 (2024):3186.
- Cancer Manag Res (2021):4709-4723.PMID:34163246
- Acs Pharmacol Transl (2024).
- Heliyon (2024).
- Food Hydrocolloid 120 (2021):106980.
- J Clean Prod 315 (2021):128201.
- Oxid Med Cell Longev 2021 (2021):1-30.PMID:34257808
- Acs Appl Opt Mater (2024).
- Transl Cancer Res 13.4 (2024).
- Front Cell Neurosci 15 (2021):569424.PMID:33815059
- Appl Entomol Zool (2022):1-14.
- Pharmaceutics 16.6 (2024):744.
- Acs Omega (2024).
- Talanta (2024):126381.
- Mol Cell Biochem (2024) 1-13.
- J Cancer 15.13 (2024):4244-4258.
- Acta Pharmacol Sin 44.1 (2023):255-257.PMID:35773339
- Nat Commun 15.1 (2024):4969.PMID:38862489
- Heliyon (2024).
- Mol Ther-Nucl Acids (2020).
- J Pharm Sci 75.10 (2020):505-509.PMID:33305726
- Phytomedicine (2024):155767.
- Biomaterials 272 (2021):120757.PMID:33798960
- Molecular Therapy Oncology (2024).
- Anal Cell Pathol 2020 (2020).PMID:33354489
- Theranostics 14.7 (2024):2687.
- Front Pharmacol 15 (2024):1389976.
- Available at SSRN 4830418.
- Front Pharmacol 11 (2021):618262.PMID:33551818
- J Biol Chem 297.2 (2021).PMID:33722608
- Phytomedicine (2024):155744.
- Front Cell Dev Biol 8 (2021):568304.PMID:33763412
- bioRxiv (2024):2024-05.
- Redox Biol (2024):103143.
- Nanoscale (2024).
- Phytomedicine (2024):155347.
- Front Immunol 15 (2024):1347683.PMID:38343537
- Antioxidants Basel 13.3 (2024):264.
- New J Chem (2024).
- Kaohsiung J Med Sci (2024).PMID:38340032
- Phytochem Lett 47 (2022):174-179.
- J Ethnopharmacol (2024):117867.PMID: 38342155
- Transl Cancer Res 11.1 (2022):206.PMID:35261897
- Tissue Cell 76 (2022).PMID:35182988
- J Biomed Nanotechnol 20.1 (2024):133-142.
- Pharmacol Res 177 (2022):106112.PMID:35122955
- Nano Today 43 (2022):101416.
- Front Immunol 15 (2024):1326137.
- Bba-Mol Cell Biol L 1867.6 (2022):159137.PMID:35247568
- bioRxiv (2024):2024-02.
- Mater Today Bio (2024):100958.
- Front Oncol 12 (2022):851300.PMID:35392243
- Cell Death Dis 15.1 (2024):97.PMID:38286802
- Oncol Lett 23.5 (2022):1-8.PMID:35350588
- J Cancer 13.6 (2022):1808.PMID:35399733
- Int J Mol Sci 25.2 (2024):919.
- Sensors & Diagnostics (2024).
- Bio-Des Manuf 5.2 (2022):358-370.
- Exp Ther Med 23.2 (2022):1-9.PMID:35069839
- J Agr Food Chem (2024).
- Int J Mol Sci 21.21 (2020):8095.PMID:33142980
- J Inflamm Res 14 (2021):3523.PMID:34335041
- Fiber Polym (2022):1-9.
- Biomed Res Int 2021 (2021).PMID:34373837
- Dis Markers 2021 (2021):1-12.PMID:34413913
- Nanomaterials 11.8 (2021):2008.PMID:34443839
- New J Chem 48.11 (2024):5068-5073.PMID:PMID
- Int J Mol Sci 22.16 (2021):8898.PMID:34445601
- Cells 10.9 (2021):2399.PMID:34572049
- Exp Ther Med 27.5 (2024):1-16.
- Nano Res (2022):1-8.
- Exp Ther Med 23.2 (2022):1-9.PMID:35069838
- Phytochem Lett 46 (2021):79-89.
- Fitoterapia (2024):105884.
- Eur J Med Chem (2024):116323.PMID:(2024) 116323.
- J Environ Pathol Tox 42 (2023).PMID:36749091
- Carbohyd Polym 277 (2022):118869.PMID:34893274
- PeerJ 9 (2021):e11482.PMID:34820154
- Tissue Cell 74 (2022):101680.PMID:34808429
- Mater Design (2024):112812.
- Nano Today 41 (2021):101326.
- J Funct Foods 114 (2024):106076.
- Int J Mol Sci 25.24 (2024):13439.
细胞计数试剂盒-8(CCK-8)使用WST-8(2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酚基)-2H-tetrazolium,单钠盐),在存在电子载体1-Methoxy PMS的情况下,产生水溶性福尔马定染料进行方便的测定。 CCK-8溶液直接加入到细胞中,无需预先混合组分。 WST-8被细胞脱氢酶生物还原为可溶于组织培养液的橙色福尔马定产物。 产生的福尔马定量与活着的细胞数量成正比。由于CCK-8溶液非常稳定且具有很小的细胞毒性,因此可以进行更长时间(例如24至48小时)的孵育。
细胞计数试剂盒 - 8允许灵敏度高、用于增殖和毒性测定中确定存活细胞数量的显色法检测。 检测灵敏度比MTT、XTT或MTS等任何其他四唑盐都要高。
图1:Cell Counting Kit-8(CCK-8)的工作机制。
细胞数量确定
1.将细胞悬浮液(100μL/孔)接种在96洞孔板中。将板在潮湿的培养箱中预孵育(例如,在37℃,5%CO2下)。
2.向板的每个孔中加入10μLCCK8溶液。注意不要在孔洞中引入气泡,因为它们会干扰O.D.读数。
3.将培养板在培养箱中孵育1-4小时。
4.使用酶标仪测量450 nm处的吸光度。
细胞增殖和细胞毒性测定
1.将种子细胞以103-104个细胞/孔的密度在96孔板中在100μL培养基中培养。将细胞在CO2培养箱中于37℃培养24小时。
2.将不同浓度的待测物质加入板中。
3.将培养板在培养箱中孵育适当的时间(例如,6,12,24或48小时)。
4.使用重复移液器向板的每个孔中加入10μLCCK8溶液。注意不要在孔洞中引入气泡,因为它们会干扰O.D.读数。
5.将培养板在培养箱中孵育1-4小时。
6.在读取孔板之前,重要的是在轨道振动器上轻轻混合1分钟,以确保颜色均匀分布。
7.使用酶标仪测量450 nm处的吸光度。
数据分析
统计分析有几种方法,您可以选择使用O.D.值或细胞数量,我们提供其中一种方法。
细胞存活率(%)= [(As-Ab)/(Ac-Ab)]×100
抑制率(%)= [(Ac-As)/(Ac-Ab)]×100
As =实验孔吸光度(含有细胞,培养基,CCK-8和待测化合物的孔的吸光度)。
Ab =空白孔吸光度(含有培养基和CCK-8的孔的吸光度)。
Ac =对照孔吸光度(含有细胞,培养基和CCK-8的孔的吸光度)。
制作标准曲线
1. 细胞计数板计数细胞悬液中的细胞数。
2. 使用培养基,等比稀释细胞悬液为一个浓度梯度,通常需要5-7个浓度梯度,每组几个复孔。然后接种细胞。(注意每孔的细胞数量。如果您将细胞悬液稀释在管中,在加入培养板的孔之前,请小心再次混匀细胞。每孔中细胞悬液的体积应该是一致的。)
3. 培养直至细胞贴壁(通常2-4小时),然后每100 μl培养基加入10 μl CCK-8。继续孵育1-4小时,用酶标仪测量450nm处的吸光度。制作出一条以细胞数为X轴坐标,O.D.值为Y轴坐标的标准曲线。
可以基于该曲线确定待测样品的细胞数。使用此标准曲线的先决条件是培养检测条件相同。
注意事项
1. 确保药物和CCK8均匀分布在培养基中。
2. 细胞增殖越多, 颜色越深; 细胞毒性越强,颜色越浅。
3. 对于贴壁细胞,每孔至少需要1000个细胞(100 μl培养基)。对于白细胞,由于灵敏度较低,每孔至少需要2500个细胞(100 μl培养基)。推荐的96孔板每孔最大细胞数为25000。如果使用24孔或6孔板进行该检测,请计算相应的每孔的细胞数,并调整CCK-8的体积,使其为每孔总液体体积的10%。
4. 因为CCK8测定是基于活细胞中的脱氢酶活性,影响脱氢酶活性的条件或化学物质可能导致实际活细胞数与使用CCK-8测定活细胞数之间有差异。
5. WST-8可能与还原剂反应生成WST-8 formazan。如果使用还原剂 (例如一些抗氧化剂)会干扰检测。如果待检测体系中存在较多的还原剂,需设法去除。
6. 孵育2小时后,背景O.D.值一般为0.1-0.2单位。
7. 注意不要在孔中引入气泡,因为它们会干扰O.D.值。
8. 如果您想对CCK8溶液进行灭菌,请使用0.2 μm的膜过滤溶液。
9. 孵育时间因孔中细胞的类型和数量而异。通常,白细胞着色较弱,因此可能需要较长的孵育时间(长达4小时)或大量细胞(~105细胞/孔)。
10. 如果细胞悬液中存在高浊度, 测量并减去样品在600nm或更高波长的O.D值。
11. CCK8不能用于细胞染色。
12. 培养基中的酚红不会影响实验结果,酚红的吸光度可以在计算时,通过扣除空白孔中本底的吸光度而消去,因此不会对检测造成影响。
13. CCK8的毒性非常低,在CCK8测定完成后,相同的细胞可用于其他细胞增殖测定,例如结晶紫测定,中性红测定或DNA荧光测定。(除非细胞极为稀少,不推荐。)
14. 该试剂盒可用于大肠杆菌,但不能用于酵母细胞。
15. 在读取平板之前,您可以在摇床上轻柔混匀。
16. 我们建议将细胞接种在靠近培养板中央的孔中,最外围一圈孔中的培养基容易蒸发,可以用PBS,水或培养基填充这些孔。
17. 如果您没有450nm滤光片。您也可以使用吸光度在430和490nm之间的滤光片, 450nm滤光片具有最佳灵敏度。
18. 测量450nm处的吸光度,如果您需要进行双波长测定,作为参考波长可以测定650nm处的吸光度。
19. 药物中金属离子的存在可能会影响CCK8的灵敏度。终浓度为1mM的氯化亚铅、氯化铁、硫酸铜会抑制 5%、15%、 90% 的显色反应,使灵敏度降低。如果终浓度是10mM的话,将会100%抑制。
常见问题
Q:如何做空白对照?
A:可以用加相应量细胞培养基和CCK-8但不加细胞的孔作为空白对照,若担心所使用的药物会干扰检测,需设置加相应量细胞培养液、药物和CCK-8但不加细胞的孔作为空白对照。
Q:只加CCK-8不加细胞的空白孔,数值上增?
A:在整个实验过程中,随着时间的增加,如果细胞培养时间较长,请注意蒸发问题。因为反应环境温度较高,会有部分液体挥发。空白组虽然没有细胞,但是随着液体的挥发,药物浓度相当于提高了,所以空白对照的数值也会有轻微的变化(但不会有明显的变化)。如果很在意空白对照的数值,可将96孔板外围一圈加培养基、水或PBS 保湿。同时,可以把96孔板置于培养箱内靠近水盘的位置以缓解蒸发。将96孔板外围两圈用PBS铺板,这样挥发先挥发周边的,可以对内部及空白对照的影响降至最低。
Q:一般多久测一次值?
A:预实验时可以在0.5、1、2和4小时后分别用酶标仪检测,然后选取吸光度范围比较适宜的一个时间点用于后续实验。
Q:加入CCK8后暂时不测OD值,可以如何处理暂时保存?能够保存多久?
A:如果需要暂时不测定OD值,可以向每孔中加入10ul 0.1M HCl溶液或者1% w/v的SDS溶液,避光保存在室温,24小时内吸光度不会发生变化。
Q:如何保存?
A:CCK-8溶液在避光、0-5℃的条件下可以保存1 年,若长期不用可在-20℃下避光保存2年。建议分装后保存于-20℃,使用时提前于4℃解冻,请避免反复冻融,否则增加背景值,干扰实验结果。
Q:是否需要更换培养基?
A:不需要,若药物影响比较小的情况可以不更换培养基,直接扣除培养基中加入药物后的空白吸收即可。
Q:待测物质有氧化性或还原性该做何处理?
A:本试剂盒的检测依赖于脱氢酶催化的反应,如果待测物质有氧化性或还原性,可在加CCK-8之前更换新鲜培养基,去掉药物的影响。
如果实验中有还原剂,请检查背景的OD值,即在不含细胞的培养基中加入药物,然后加入CCK-8试剂在一定时间内检测,和不加药物的培养基进行比较(只有CCK-8试剂),如果O.D值明显偏高,则说明有反应。
Q:如果培养基颜色或PH已变化,是否需要更换新的培养基?
A:加入CCK-8时,如果细胞培养时间较长,培养基颜色或pH值已变化,建议换用新鲜的培养基。
Q:适用细胞?
A:因为测线粒体酶,所以真核细胞,有线粒体的均可,例如单细胞,动物细胞。但有细胞壁的细胞,例如植物细胞,酵母细胞,底物进入细胞效率低,不推荐。
Q:线性不佳原因?
A:细胞不均;培养液蒸发;还原剂;气泡;孔板周围一圈不能用,除非只培养少于一天的时间;细胞数目很重要,太少信号会低。
Q:金属毒性?
A:可以检测金属毒性,最好做空白对照。
Q:适用于那些细胞?其他还有哪些细胞可用?生物被膜内的活菌 (流感嗜血杆菌和绿脓杆菌)?
A:所有哺乳动物的细胞,细菌一般没有检测CCK8的,可以染色法,或者流式,最常规的方法,有报道, 比浊法测OD600。
Q:是否可以替代Brud或EDU法检测细胞增殖?
A:虽然CCK8通过检测对生长期的细胞内的脱氢酶来反应细胞的活性,而胸腺嘧啶插入法通过核苷酸类似物的方式插入合成的DNA来检测细胞的活性,这几种方法是有一定关联性的,CCK8可替换胸腺嘧啶插入法来检测细胞增殖,并且检测更方便。
Q:空白孔数值偏高,有可能是培养基长菌吗?或者其他原因?
A:空白孔数值偏高一般认为染菌造成的,很少出现这种问题。
Q:CCK8能检测组织吗
A:不能检测组织。
Q:是否可以检测T细胞
A:可以,T细胞长的慢,需延长孵育时间。
Q:当两个细胞混合培养时(骨髓间充质细胞,另一个是悬浮T细胞),培养一段时间后,只测骨髓间充质干细胞的生长、增殖情况,请问用CCK-8能测吗?怎么做?
A:对于两种细胞的情况:
1)如果测定贴壁细胞的生长状况,那么可以把悬浮的T细胞转移掉,使用上述第二种方法测定。
2)如果T细胞对于间充质细胞的生长很关键,不能取走,那么测定的活性程度是总量,此时,需要对每一个孔的T细胞进行估算,如果每个培养条件下T细胞总量接近,或者T细胞含量很少,远远小于间充质细胞,也可以测定,使用对照孔可以去除T细胞的影响。
3)如果T细胞可以短时间内除去,可以找个灵敏度高的CCK-8,信号强,建议细胞量合适的情况下(每一个孔10000个),30分钟左右出读数结果,测 定后,可以将T细胞再加入培养孔。 这样做对细胞的生长影响不大。但是如果做动力学,那么要考虑的因素多一些。
Q:CCK-8是通过和细胞内的脱氢酶反应而反映着细胞数量,假如细胞已经死亡,而脱氢酶活性还有,那么如何计算细胞数量?
A:多数情况下,细胞死亡过程中,细胞内的成分会降解,脱氢酶会有部分释放,但是活性和活细胞比,比较低。举例说:100个细胞死亡20小时后,释放的脱氢酶活力,不足5个活细胞的脱氢酶活力,也就是说,这种情况带来的误差5%以内。而且,这种细胞死亡,对同种细胞来说,情况是一样的,一般都是在不同的孔间比较同种细胞,所以系统误差是一样的,即系统误差会彼此消减。
应用&特点 |
1) 细胞增殖测定 - GlpBio细胞计数试剂盒-8(CCK-8)是水溶性的,在培养中是稳定的,并且是无毒的。 2) 细胞活力测定 - 代谢活性和染料产生与改变的活力成比例地变化。 3) 细胞因子测定 - 测量细胞因子诱导的增殖。如果需要,可以在研究结束时回收细胞并扩增。 4) 细胞毒性测定 - 来自细胞毒性化学物质的细胞死亡对颜色发展没有影响,只有活细胞将试剂转化为比色指示剂。试剂本身具有可忽略的毒性,并且通常对细胞是安全的。 |
运输方式 | 蓝冰运输。 |
储存条件 | 储存在4°C避光,可稳定长达12个月。储存在-20°C避光,可稳定长达2年。 |
用途 | 仅供研究使用!不能用于人体。 |
CCK-8 | MTT | MTS | SRB |
Solubility | Water soluble | Indissolvable | Water soluble | Indissolvable |
Detection Wavelength | 450nm | 490nm | 450nm | 510nm |
Character | Liquid | Solid | Liquid | Liquid |
Usage | No need to prepare | Prepare the solutions | Use it right after it was ready | Prepared beforehand |
Need to redissolve or not | NO | Yes,by DMSO | No | Yes,by Tris-base solution |
Convenience | +++ | ++ | +++ | + |
Detection speed | +++ | + | ++ | + |
Repeatability | +++ | + | ++ | ++ |
Stability | ++ | + | + | +++ |
-
Related Biological Data
Therapeutic targeting of the DDR1–NF-κB–NRF2 axis inhibits PDAC growth and metabolism. a, Parental and E79Q KPC cells plated on wild-type or R/R ECM were incubated in LG medium with or without 7rh, ML120B or ML385. Total viable cells are presented relative to parental cells that were treated with vehicle and plated on wild-type ECM.
Cell viability was determined with a Cell Counting Kit-8 assay (Glpbio).
Nature 610.7931 (2022): 366-372. PMID: 36198801 IF: 69.5026 -
Related Biological Data
LIG1 reversed cell proliferation, apoptosis and DNA damage effects of hsa_circ_0007919 (A-B) CCK-8 analysis of the proliferation of GEM-resistant PDAC cells with or without LIG1 inhibition.
EIPA , IPI549, MBQ-167, MRT68921 or erlotinib, ML385, HCQ or their combinations were added to the wells for 24, 72 or 96 hrs. Next, 10 uL of CCK-8 (GlpBio) was added to each well.
Cancer Cell (2021). PMID: 33740421 IF: 38.5836 -
Related Biological Data
Effect of CrEL on glucose transport.Cell Counting Kit-8 (CCK-8) is a fluorescent glu-cose analog. INK-128 (mTORi) was used as a control for de-creased glucose transport.
Cells after transfection or GEM treatment were collected and counted, then 4 × 103 cells were placed into 96-well plate and cultured in incubator at 37°C. 24, 48, 72 and 96 h after, cells were incubated with 100 μl serum-free medium and 10 μl CCK-8 solution (Glpbio, USA) at 37°C for 2 h and measured at 450 nm wavelength.
Molecular Cancer, 2023, 22(1): 195. PMID: 38044421 IF: 37.2999 -
Related Biological Data
Bifidobacterium cultural supernatant and I3C protected mice against ALF. (G-H) Primary hepatocytes were pre-treated with or without I3C for 1 h followed by PBS or APAP administration for 12 h. The effects of I3C on CCK8 (G) and LDH (H) were detected. n=3-5.
Cell counting kit 8 (CCK8) experiment for in vitro assay were performed using the CCK8 Kit (Glpbio, Cat# GK10001, USA) according to the manufacturer's instructions.
Cell Host & Microbe 32.1 (2024): 48-62. PMID: 38056458 IF: 30.3013 -
Related Biological Data
Fe3O4@IR820@CpG induces immunogenic cell death and promotes DC maturation in vitro. A,B) Viability of MC38 cells after incubation with A) different concentrations of Fe3O4@IR820@CpG without laser irradiation or B) different formulations with 808 nm laser irradiation (1 W cm−2) for 5 min, as detected by CCK-8 kit (n = 4).
Bicinchoninic Acid Assay (BCA) protein assay kit,cell counting kit-8 (CCK-8), and erythrocyte membrane lysates were purchased from GlpBio (Montclair, USA).
Advanced Materials, 2023: 2307193. PMID: 37951210 IF: 29.4006 -
Related Biological Data
H) The viability ofMC38 cells treated with PAD@MS for 48 h in the presence or absence of 10 μM Ferr-1, 20 μM Nec-1, 10 μM z-VAD-FMK, and 1 mM NAC.
Cell viability was detected by cell counting kit-8 (CCK-8) assay (GlpBio, USA) according to the manufacturer’s instruction.
Adv Funct Mater, 2023: 2214998. IF: 19.9246 -
Related Biological Data
After transfection with si-1 and si-2, performed CCK-8 assays were performed to evaluate the cellular growth curves and the results revealed that repressing the expression of PCAT6 remarkably suppressed the cell proliferation of HeLa and SiHa cells.
Each well was added with 10 μl CCK-8 reagent (GLPBIO, Montclair, CA, USA) and the plates were continued to be cultured for 1-2 h. The cell viability was determined by measuring the optical density (OD) absorbance at the wavelength of 450 nm.
Eur Rev Med Pharmacol Sci.2019 Mar;23(5):1947-1956 -
Related Biological Data
Blocking bFGF leads to decrease in cell proliferation and clone formation of cisplatin (DDP) selected A549 cells. Growth curves showed that knocking down bFGF expression resulted in reduction in cell proliferation.
The cells were incubated for 24 h in incubator, and then,10μl CCK-8 (GLPBIO) was added in every well and cultivated at room temperature for 4 h. Absorbance was measured at 450 nm. The experiment repeated three times successive 7 days.
J Pharm Pharmacol.2019 Sep;71(9):1412-1420.