Phosphatase Inhibitor Cocktail I (100× in DMSO)
目录号 : GK10011磷酸酶抑制剂混合液I（Phosphatase Inhibitor Cocktail I (100× in DMSO) ）有效抑制碱性磷酸酶和丝氨酸/苏氨酸蛋白磷酸酶，如PP1和PP2A。
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).
Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Components
| Ingredient | M.W. | 100x Conc. | Target |
| (-)-p-Bromotetramisole oxalate | 995.2 | 2.5 mM | Alkaline phosphatases |
| Cantharidin | 373.2 | 500 μM | Ser/Thr phosphatases |
| Microcystin LR, Microcystis aeruginosa | 196.2 | 500 nM | PP1 and PP2A |
磷酸酶抑制剂混合物I（Phosphatase Inhibitor Cocktail I）有效抑制碱性磷酸酶和丝氨酸/苏氨酸蛋白磷酸酶，如PP1和PP2A。蛋白质和脂质的磷酸化是细胞信号或代谢状态的标志。三种抑制剂的混合物，可抑制碱性磷酸酶以及丝氨酸/苏氨酸蛋白磷酸酶，如PP1和PP2A。每个小瓶含有1ml磷酸酯酶抑制剂混合物，该混合物溶解在DMSO中，含有以下组分：草酸盐溴特氨酰亚胺（2.5 mM）、斑蝥素（500µM）和铜绿微囊藻毒素LR（500 nM）。磷酸酶抑制剂混合物I已针对哺乳动物细胞和组织提取物进行了优化和测试。