Protease Inhibitor Cocktail (EDTA-Free,100× in DMSO)

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of inhibitors of those enzymes known to be present.

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates.

Protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. This cocktail is supplied as a ready-to-use solution in DMSO.

内源性蛋白质会在平衡状态下产生并降解，因此在稳定的环境条件下，其细胞水平是稳定的。粗细胞提取物含有许多内源酶，如磷酸酶和蛋白酶，它们能够降解提取物中的蛋白质。当在体外从细胞和组织中提取蛋白质时，蛋白质产生被显著抑制，降解增加。提高完整蛋白产量的最佳方法是添加已知酶的抑制剂。蛋白酶抑制剂混合物（Protease Inhibitor Cocktail）用于植物细胞裂解物或组织提取物中，以增加蛋白质的稳定性。该混合物的功能是抑制蛋白酶降解非磷酸化或磷酸化的蛋白质底物。这种蛋白酶抑制剂混合物包含单独的成分，包括AEBSF、Aprotinin、Bestatin、E-64、Leupetin和Pepstatin A，对半胱氨酸、丝氨酸、天冬氨酸、金属蛋白酶和氨肽酶具有广泛的特异性。这种蛋白酶抑制剂已经针对植物细胞和组织提取物进行了优化和测试。该蛋白酶抑制剂混合物以DMSO中的即用型溶液形式提供。